Grasas y Aceites, Vol 44, No 6 (1993)

Fatty acid determination from microalgal biomass


https://doi.org/10.3989/gya.1993.v44.i6.1069

J. L. García Sánchez
Departamento de Ingeniería Química. Facultad de Ciencias Experimentales. Universidad de Almería, Spain

E. Molina Grima
Departamento de Ingeniería Química. Facultad de Ciencias Experimentales. Universidad de Almería, Spain

F. García Camacho
Departamento de Ingeniería Química. Facultad de Ciencias Experimentales. Universidad de Almería, Spain

J. A. Sánchez Pérez
Departamento de Ingeniería Química. Facultad de Ciencias Experimentales. Universidad de Almería, Spain

A. Giménez Giménez
Departamento de Ingeniería Química. Facultad de Ciencias Experimentales. Universidad de Almería, Spain

Abstract


Long chain polyunsaturated fatty acids are focusing the interest of researchers as long as analytical techniques are being improved. The Lepage and Roy direct transesterification method is being widely used to measure fatty acid content of microalgae which are proposed as potential sources of these products. In this paper, the physical state of the biomass to be analyzed is studied. Comparison has been stated between lyophilized biomass and wet biomass just harvested. Furthermore, the amount of sample and the number of methyl ester extractions have also been studied. From the statistical analysis carried out, wet biomass to fatty acid determination and the adequate range of sample are proposed. Three methyl ester extractions improve the resolution of GC analysis.

Keywords


Fatty acid (determination); Microalgae; Physical state (comparison); Polyunsaturated fatty acid

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