Grasas y Aceites, Vol 56, No 2 (2005)

Effect of cell immobilization on the treatment of olive mill wastewater by a total phenols, acetic acid and formic acid degrading bacterium strain

Abdelghani El Asli
School of Science & Engineering, Al Akhawayn University, Morocco

Faouzi Errachidi
Faculté des Sciences, Université Sidi Mohamed Benabdellah, Morocco

Rhizlane Bennisse
3Faculté des Sciences et Techniques, Université Cadi Ayyad, Morocco

Abdel-illah Qatibi
3Faculté des Sciences et Techniques, Université Cadi Ayyad, Morocco

Mohamed Errami
Faculté des Sciences, Université Abdelmalek Saadi, Morocco


Olive mill wastewater (OMW) is a pure vegetative by-product, containing a high organic and polyphenol content and is resistant to biodegradation. Its disposal lead to major environmental pollution problems in the Mediterranean basin. An aerobic bacterium was isolated from OMW. During three consecutive diluted and supplemented OMW treatment cycles, significant abatement of its phytotoxic substances was observed. In fact, total phenols, acetic and formic acids were reduced between 33 and 64 % when cells of the isolated bacterium were grown free; and between 62 and 78 % when cells of the same isolated bacterium were grown immobilized in a polyurethane sponge. These results suggest that the bacterium culture of the new isolate would decrease the OMW phytotoxicity. Phylogenetic analysis of 16S ribosomal DNA showed that all the related sequences are members of the Enterobacteriaceae family and revealed that the isolated bacterium was characterized as a Klebsiella oxytoca strain.


Aerobic degradation; Immobilization; Klebsiella oxytoca; Olive mill wastewater; Total phenol

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