Grasas y Aceites, Vol 62, No 1 (2011)

Antioxidant effect of an ozonized theobroma oil formulation on damaged-inflammatory rat skin


https://doi.org/10.3989/gya.055710

Yaima Sánchez
Ozonized Vegetable Oils Group. Ozone Researches Center. CNIC, Cuba

Maritza F. Díaz
Ozonized Vegetable Oils Group. Ozone Researches Center. CNIC, Cuba

Frank Hernández
Ozonized Vegetable Oils Group. Ozone Researches Center. CNIC, Cuba

Dayana Gil
Ozonized Vegetable Oils Group. Ozone Researches Center. CNIC, Cuba

Gastón García
LIORAD Control Group of Biomedical Researches Laboratory, Cuba

Abstract


The aim of this study was to determine whether a cosmetic formulation elaborated with ozonized theobroma oil may exert beneficial effects in the restoring of the antioxidant activity on the skin of rats previously irradiated with ultraviolet light. 0.5 g of the formulation was applied on the skin of rats for five days. Superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activity were determined in a homogenate of rat skin. Malondialdehyde (MDA), conjugated dienes (CD) and total hydroperoxide (THP) content were determined as biomarkers of oxidative stress. Using these parameters, antioxidant and oxidant activity, redox index and oxidative stress grade were determined. The total antioxidant activity was significantly increased while the redox index, total oxidant activity and oxidative stress grade decreased significantly in damaged rats treated with the formulation. These results show the antioxidant properties of the cosmetic formulation due to the stimulation of antioxidant enzymes such as SOD and GPx, preventing skin injury induced by ultraviolet irradiation.

Keywords


Antioxidant activity; Cosmetic formulation; Oxidant activity; Oxidative stress; Ozonized theobroma oil

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References


Álvarez A. Consecuencias del estrés oxidativo en la piel por radiaciones ultravioleta. 1995. Rev. Cubana Invest. Biomed. 14, ene-dic.

Bocci V. 2005. Ozone a new medical drug. Springer, Netherlands.

Calabuig PA, Gutiérrez T. 2004. Composiciones que contienen aceites naturales ozonizados. EPO 2 211 341, A61K 31/327.

Calunga JL, Zamora ZB, Borrego A, Río S, Barber E, Menéndez S. 2005. Ozone Therapy on Rats Submitted to Subtotal Nephrectomy: Role of Antioxidant System. Mediat. Inflamm. 4, 221–227. doi:10.1155/MI.2005.221 PMid:16192672    PMCid:1526476

Díaz M, Martínez G, Arteaga M, Garcés R. 2006a. Chemical Analysis of Ozonized Theobroma Fat. J. Am. Oil Chem. Soc. 83, 1-4.

Díaz M, Hernández R, Martínez G, Vidal G, Gómez M, Fernández H, Garcés R. 2006b. Comparative Study of Ozonized Olive Oil and Ozonized Sunflower Oil. J. Brazil Chem. Soc. 7, 403-407.

Díaz M, García K, Sánchez Y, Hernández F, García L, Meneau RI, Gómez M, Fernández LA, Sánchez E, Gil D, García G, Piña Y, Díaz A, Rosabal M, Manet BM, Nicolás PD. 2008. Registro sanitario Crema Suavizante, Marca: Ozonol No. 074/08, Tomo: LX y Folio: 074.

Díaz M, Hernández F. 2008. Estrés oxidativo en el envejecimiento. Uso de las sustancias ozonizadas. Monografía.com http:/www.monografías.com/trabajos57/estrés-oxidativo/estrés-oxidativo.shtml.

EEC. 1979. Methods for the determination of toxicity. Council Directive 79/831/EEC, 18 September, Annex V, Part B, Annex VII, 4.1.5.

Faraji B, Kang HK, Valentine JL. 1987. Methods compared for determining glutathione peroxidasa activity in blood. Clin. Chim. Acta 33, 539-543.

Gerard-Moonier D, Erdelmeier I, Regnard K, Moze-Henry N, Yadan JC, Chaudiere J. 1998. Reactions of 1-methyl-2-phenylindone with malondialdehyde and 4-hydroxyalkenals. Analytical applications to a colorimetric assay of lipid peroxidation. Chem. Res. Toxicol. 11, 1176-1183. doi:10.1021/tx9701790 PMid:9778314

Guanche D, Hernández Frank, Zamora Z, Alonso Y. 2009. Sistema de diagnóstico computarizado para la clasificación del estrés oxidativo en un modelo de sepsis peritoneal en ratas. Rev. CENIC C. Biol. 40, 83-87.

Hasbún S, Díaz de la Roche A, Díaz M. 2005. Utilización de una crema cosmética ozonizada en la involución cutánea. Tesis Presentada por el Título de Especialista en Dermatología. Ciudad de La Habana. Cuba.

Hernández F. 2006. Registro del Sistema para el Diagnóstico del Estrés Oxidativo. Registrado en el Centro de Derecho de Autor de la Republica de Cuba, 1313.

Hernández F. 2007. To What Extent Does Ozone Therapy Need a Real Biochemical Control System? Assessment and importance of oxidative stress. Arch. Med. Res. 38, 571-578. doi:10.1016/j.arcmed.2007.03.002 PMid:17560465

Lowry O, Rosebrough H, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265-275. PMid:14907713

Martínez G, Candelario E, Menéndez S, Lamberto R, Giulianni A, León OS. 2001. Ozone Treatment Reduces Markers of Oxidative and Damage Endothelial in an Experimental Diabetal Model in Rats. Pharmacol. Res. 44, 391-396. doi:10.1006/phrs.2001.0867 PMid:11712870

Menéndez S, González R, Ledea O, Hernández F, Díaz M. 2008. Ozonoterapia aspectos básicos y sus aplicaciones clínicas. Editorial CENIC. Ciudad Habana, Cuba.

Minami M, Yoshikawa HA. 1979. Simplified assay method of superoxide dismutase activity for clinical use. Clin. Chim. Acta 92, 337-342. doi:10.1016/0009-8981(79)90211-0

Molerio J, Menéndez S, Ledea O, Díaz M, Rubí D, Fernández A, Lezcano I. 2005. Method for obtaining ozonized oils and vegetable fats and use of said products for pharmaceutical and cosmetic purposes. Sudáfrica No. 2004/8856, PCT/CU03/00003. WO 03/085072 A1.

Mulero M, Romeo M, Giralt M, Folch J, Nogues MR, Fortuno A, Sureda FX, Linares V, Cabre M, Paternain JL, Mallol J. 2006. Oxidative stress-related markers and langerhans cells in a hairless rat model exposed to UV radiation. J. Toxicol. Environ Health A 14, 1371-8. doi:10.1080/15287390500471187 PMid:16760142

Packer L. 1994. Vitamin E is nature’s master antioxidant. Sci. Med. 1, 54-63.

Pallack RL, Morse, D.R. 1988. Free radicals and antioxidants: relation to chronic disease and aging. Int. J. Phychosom 35, 43-48.

Southorn PA, Powis G. 1988. Free radicals in medicine. I: Chemical Nature and Biological Reactions. Mayo Clin. Proc. 63, 381-389. PMid:2832662

Statistic. 2003. Release: 6 Copyright Statsoft. Inc: All rights reserved.

Vanlente F, Pepoy M. 1990. Coupled-enzyme determination of catalasa activity in erythrocytes. Clin. Chim. Acta 36, 1339-1343.

Vasankari T, Kujala U, Heinonen O, Kapanen J, Aqhotupa M. 1995. Measurement of serum lipid peroxidation during exercise using three different methods: diene conjugation. thiobarbituric acid reactive material and flouroscent chromolipids. Clin. Chim. Acta 234, 63-69. doi:10.1016/0009-8981(94)05976-Y

Wolff SP. 1994. Ferrous ion oxidation in the presence of the ion indicator xylenol orange for the measurement of hydroperoxides: the FOX assay. Method Enzymol. 233C, 182-189. doi:10.1016/S0076-6879(94)33021-2

Zamora Z, Borrego A, López OY, Delgado R, González R, Menéndez S. 2005. Effects of Ozone Oxidative Preconditioning on TNF-α Release and Antioxidant- Prooxidant Intracellular Balance in Mice During Endotoxic Shock. Mediat. Inflamma 1, 16-22. doi:10.1155/MI.2005.16 PMid:15770062    PMCid:1482874




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