Grasas y Aceites, Vol 62, No 2 (2011)

Antioxidant activity, total phenolic, and total flavonoid of extracts from stems of Jasminum nervosum Lour


https://doi.org/10.3989/gya.066810

Lini Huo
College of Pharmacy, Guangxi Traditional Chinese Medical University, China

Rumei Lu
College of Pharmacy, Guangxi Traditional Chinese Medical University, China

Peiyuan Li
College of Pharmacy, Guangxi Traditional Chinese Medical University, China

Yanfang Liao
Guangxi Research Institute of Chemical Industry, China

Rui Chen
Guangxi Grain and Oil Scientific Institute, China

Chaocheng Deng
College of Pharmacy, Guangxi Traditional Chinese Medical University, China

Chengsheng Lu
College of Pharmacy, Guangxi Traditional Chinese Medical University, China

Xiangyong Wei
College of Pharmacy, Guangxi Traditional Chinese Medical University, China

Yaohua Li
College of Pharmacy, Guangxi Traditional Chinese Medical University, China

Abstract


Guangxi traditional Chinese Medical University Universidad de Medicina Tradicional China de Guangxi This study evaluated the antioxidant activities of the extracts of Jasminum nervosum Lour. stems along with the effects of different extract solvents on total phenolics (TP), total flavonoids (TF), and antioxidant potential. The antioxidant activity of the extracts was assessed using the following methods: DPPH, ABTS+ both free radicals scavenging assays, and reducing assays. TP and TF were detected by spectrophotometric and HPLC methods. In former methods, the highest amount of TP content was ethy lacetate extract (EAE), expressed as gallic acid equivalents. The greatest TF content was in the n-butanol extract (BE), expressed as lutin equivalents. No significant difference was observed in the TP/TF content between these two extracts. The antioxidant activity and TP/TF content of three extracts seemed to follow the same trend. This implied that there is a good correlation between antioxidant activities and TP/TF content. But in HPLC methods, EAE contained the highest content of lutin and gallic acid, which decreased in the same order of EAE > BE > PE, the rank order of TP/TF content of EAE and BE were different according to antioxidant ability. The overall results showed that the EAE and BE were richer in phenolics and flavonoids than petroleum ether extract (PE), and may represent a good source of antioxidants.

Keywords


Antioxidant activity; Free radical scavenger; HPLC; Jasminum nervosum Lour; Total flavaonoid and total phenolic

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