Grasas y Aceites, Vol 62, No 2 (2011)

Characterization of lipids and fatty acids of the soil derived fungus Cladosporium sp.

Bijay Krishna De, Shikha Verma

DOI: http://dx.doi.org/10.3989/gya.090910

Abstract


The fungus Cladosporium sp. was explored for its potential ability to produce lipids, fatty acids and protein. The lipids were characterized by the quantification of acylglycerols and fatty acids. Alterations in lipid and protein accumulation were observed by changing the growth medium, carbon source type (glucose and dextrose) and content, growth period and by developing salt stress through the incorporation of NaCl in the formulation of the medium. Lipid content was found to vary from 6.8 ± 0.3 to 27.3 ± 2.8%, w/w. The major fatty acids accumulated were palmitic, oleic and linoleic. The lipid fraction obtained from a Potato- dextrose medium (containing 2% dextrose and 4% NaCl) accumulated 73.7 ± 3.7%, w/w, oleic acid. Some lipid fractions were found to have fatty acid ratios like saturated, monounsaturated, and polyunsaturated close to 1:1:1 w/w/w. Triacylglycerol was obtained as the major constituent of lipid fractions.

Keywords


Cladosporium sp.; Fatty acid profile; Lipid content

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References


Industrial Waxes & Their Formulations. 1996. SBP Board of consultants & Engineers. Small Business Publications, SBP Building, 4/45, Roop Nagar Delhi.

Bajpai P, Bajpai PK, Ward OP. 1991. Effects of aging Mortierella mycelium on production of arachidonic and eicosapentanoic acids. J. Am. Oil Chem. Soc. 68, 775-780. doi:10.1007/BF02662171

Shimizu S, Kawashima H, Shinmen Y, Akimoto K and Yamada H. 1988. Production of eicosapentanoic acid by Mortierella fungi. J. Am. Oil Chem. Soc. 65, 1455- 1459. doi:10.1007/BF02898307

Saxena V, Sharma CD, Bhagat SD, Saini VS and Adhikari DK. 1998. Lipid and fatty acid biosynthesis by Rhodotorula minuta. J. Am. Oil Chem. Soc. 75, 501-505. doi:10.1007/s11746-998-0254-x

Chaudhuri S, De BK and Bhattacharyya DK. 1997. Essential fatty acids of lipids produced by soil around oil extraction mill. J. Oil Technol. Assocn. of India. 29, 35-38.

De BK and Kumar T. 2005. Production and optimization of polyunsaturated fatty acids in some fungi of Aspergillus and Mucor Genus. J. Oil Technol. Assocn. of India. 37, 8-13.

Naqvi BS, Hashmi K, Khan FA, Sheikh D and Mehmood ZA. 1997. Production of lipids by fermentation preliminary report. J. Islamic Academy of Sciences. 10, 13-18.

Bhatia IS, Raheja RK, Chahal DS. 1972. Fungal lipids. I. Effect of different nitrogen sources on the chemical composition. J. Sci. Food and Agric. 23, 1197-1205. www.telegraphindia.com/1090202/jsp/atleisure/story_10472623.jsp doi:10.1002/jsfa.2740231007 PMid:4673839

Kaushik N and Agnihotri A. 2000. GLC analysis of Indian rapeseed- mustard to study the variability of fatty acid composition. Biochemical Society Transactions. 28, 581- 583. doi:10.1042/BST0280581 PMid:11171132

Cappuccino JG, Sherman N. 1999. In Microbiology- A Laboratory Manual, fourth ed. Addison- Wesley Longman, Inc. Harlow, England

Metacalfe LD and Schmitz AA. 1961. The rapid preparation of fatty acid esters for gas chromatographic analysis. Anal. Chem. 33, 363 - 364 doi:10.1021/ac60171a016

Sadasivam S and Manickam A. 2004. In Biochemical Methods, second ed. New Age International (P) Ltd. New Delhi Kates M. 1972. Techniques of lipidology. (Work, T.S.; Work, E. ed.) American Elsevier publishing Co., Inc. New York

Chen PS, Toribara TY and Warner H. 1956. Microdetermination of phosphorus. Anal. Chem. 28, 1756 -1758 doi:10.1021/ac60119a033

Furniss BS, Hannaford AJ, Smith PWG, Tatchell AR. 2004. In VOGEL’S Textbook of Practical Organic Chemistry, fifth ed. Pearson Education (Singapore) Pte. Ltd.

Fujii S, Uenaka M, Nakayama S, Yamamoto R, Mantani S. 2001. Effect of sodium chloride on the fatty acid composition in Boekelovia hooglandii (Ochromonadales, Chrysophyceae). Phycological Research. 49, 73-77. doi:10.1111/j.1440-1835.2001.tb00235.x

Yuan KL, Hai MT, Ching SL. 1989. Effect of salinity of medium on cellular fatty acid composition of marine alga Porphyridium cruentum (Rhodophyceae). J. Appl. Phycology. 1, 19-23. doi:10.1007/BF00003531

Floreto EAT, Hirata H, Yamasaki S and Castro SC. 1994. Effect of salinity on the growth and fatty acid composition of Ulva pertusa Kjellman (Chlorophyta). Botanica Marina. 37, 151-155. doi:10.1515/botm.1994.37.2.151




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