Grasas y Aceites, Vol 63, No 4 (2012)

Physicochemical characteristics of ozonated sunflower oils obtained by different procedures


https://doi.org/10.3989/gya.073212

M. F. Díaz
Ozone Research Center, Cuba

Y. Sánchez
Ozone Research Center, Cuba

M. Gómez
Ozone Research Center, Cuba

F. Hernández
Pharmacy and Food Institute from Havana, Cuba

M. C. da C. Veloso
Chemical Institute-Federal University of Bahia, Brazil

P. A. de P. Pereira
Chemical Institute-Federal University of Bahia, Brazil

A. S. Mangrich
Chemical Institute-Federal University of Parana, Brazil

J. B. de Andrade
Chemical Institute-Federal University of Bahia, Brazil

Abstract


Two ozonation procedures for sunflower oils at different applied ozone dosages were carried out. Ozone was obtained from medicinal oxygen and from air. Peroxide, acidity, and iodine indexes, along with density, viscosity and antimicrobial activity were determined. The fatty acid compositions of the samples were analyzed using GC. The content of oxygen was determined using an elemental analysis. Electronic Paramagnetic Resonance was used to measure the organic free radicals. The reactions were achieved up to peroxide index values of 658 and 675 mmolequiv kg–1 using medicinal oxygen and air for 5 and 8 hours, respectively. The samples of ozonized sunflower oil did not present organic free radicals, which is a very important issue if these oils are to be used as drugs. The ozonation reaction is more rapid with medicinal oxygen (5 hours) than with air (8 hours). Ozonized sunflower oil with oxygen as an ozone source was obtained with high potential for antimicrobial activity.

Keywords


Acidity index; Antimicrobial activity; Elemental Analysis; Gaseous Chromatography; Iodine index; Ozonized sunflower oil; Peroxides index

Full Text:


PDF

References


Bailey P. 1978. Ozonolysis of Olefins. Academic Press, New York.

British Pharmacopeia. 2000a. Peroxide Value, Appendix XF, IA, London.

British Pharmacopoeia. 2000b. Acid value, Appendix XB, IA, IB, London.

British Pharmacopoeia. 2000c. Iodine value, Appendix XE, IA, IB, London.

British Pharmacopeia. 2000d. Determination of weight per mililitre, density, relative density and apparent density, Appendices V G, London.

British Pharmacopeia. 2000e. Determination of Viscosity, Appendix VH, London.

Chevrier N, Fathey S, Young S. 1988. Oxidative damages and repair in Euglena gracilis exposed to ozone. I. SH groups and lipids. Plant Cell Physiol. 29, 321-327.

Criegee R. 1975. Mechanism of Ozonolisis, 14 Ed. Angewandte Chemie Int, England.

Curtiellas V, Gómez M, Ledea O, Fernández I, Sánchez E. 2005. Actividad antimicrobiana del OLEOZON® sobre Staphylococcus aureus y Pseudomonas aeruginosas. Rev. CENIC Ciencias Biol 36, No. Especial.

Díaz M, Gavín JA, Ledea O, Hernández F, Alaiz M, Garcés R. 2005. Spectroscopic chracterization of ozonated sunflower oil. Ozone Sci. Eng. 27, 247-253. http://dx.doi.org/10.1080/01919510590945822

Díaz M, Hernández R, Martínez G, Vidal G, Gómez M, Fernández H, Garcés R. 2006. Comparative study of ozonized olive oil and ozonized sunflower oil. J. Brazil Chem. So. 17, 403-407. http://dx.doi.org/10.1590/S0103-50532006000200026

Díaz M, Martínez G, Gavín JA, Meneau RI, Fernández I. 2007. Estudio de la grasa de teobroma ozonizada usando la Resonancia Magnética Nuclear protónica y análisis microbiológico. Grasas Aceites 58, 15-19.

Díaz M, Gavín JA, Andrade JB. 2008. Structural characterization by Nuclear Magnetic Resonance of ozonized triolein. Grasas Aceites 59, 274-281.

Díaz M, Ledea O, Gómez M, Garcés R, Alaiz M, Martínez-Force E. 2009. Estudio comparativo de la ozonización de aceites de girasol modificados genéticamente y sin modificar. Quim Nova 32, 2467-2472. http://dx.doi.org/10.1590/S0100-40422009000900041

Díaz M, Meneau RI, García G, Sánchez Y, García K. 2010. Evaluación del poder irritante del jabón bactericida BELLOIL® Rev CENIC Ciencias Biol, Suplemento Especial.

Firestone D. 1999. Physical and chemical characteristics of oils, fats and waxes. Ed AOCS Press, EU.

Garcés R, Mancha M. 1993. One-Step lipid extraction and fatty acid methyl esters preparation from fresh plant tissues. Anal Biochem. 211, 139-143. http://dx.doi.org/10.1006/abio.1993.1244 PMid:8323025

Geeraert E, Shepeer D. 1982. Structure elucidation of triglycerides by chromatographic techniques. Part 1 Capillary GC of ozonized triglycerides. J. High Res. Chromatog. Commun. 5, 80-84. http://dx.doi.org/10.1002/jhrc.1240050204

Gunstone F, Haewood L, Padley B. 1994. The lipid handbook. Chapman. Hall Ed, London.

Ledea O. 2003. Estudio de la composición química del aceite de girasol ozonizado OLEOZON®. Tesis Presentada en Opción al Grado Científico de Doctor en Ciencias Químicas. Ciudad de La Habana, Cuba. PMid:12482495

Hernández F, Hernández D, Zamora Z, Díaz M, Ancheta O, Rodríguez S, Torres D. 2009. Giardia duodenalis: Effects of an ozonized sunflower oil product (Oleozon®) on in vitro trophozoites. Exp. Parasitol 121, 208-212. http://dx.doi.org/10.1016/j.exppara.2008.10.009 PMid:19010325

Komanapalli IR, Mudd JB, Lau HS. 1997. Effect of ozone on metabolic activities of Escherichia coli K-12. Toxicol Lett. 90, 61-66. http://dx.doi.org/10.1016/S0378-4274(96)03830-1

Lezama TR. 1997. Espectroscopia de Resonancia Paramagnética Electrónica. Servicio Editorial Euskal Herriko Unibertsitatea, Argitarapen Zerbitzua, Universidad del País Vasco.

Martínez G, Hernández R, Díaz M. 2005. Determinación de hidroperóxidos totales en aceite de girasol ozonizado mediante el método de oxidación ferrosa en xilenol naranja. Grasas Aceites 56, 147-152.

Molerio J, Menéndez S, Ledea O, Díaz M, Rubí D, Fernández A, Lezcano I. 2009. Procedimiento para la obtención de aceites y grasas vegetales ozonizados para fines farmacéuticos y cosmetológicos. CU 23467, Resolución No. 2974, La Habana. NC norma cubana. 1998. Aceites vegetales refinados comestibles 5. Especificaciones. 1ra Edición Oficina Nacional de Normalización.

NCCLS. 1999. National Committee for Clinical Laboratory Standards. Method for Determining Bactericidal Activity of Antimicrobial Agents. Approved guideline, 19(18).

NCCLS. 2000. National Committee for Clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility, Testing of anaerobic bacteria that grow aerobically. Approved Standard, Fifth edition, 20 (2).

Pasto D, Johnson C. 1980. Determinación de estructuras orgánicas. Pueblo y Educación ED, Ciudad de la Habana.

Robards K, Kerr A, Patsalides E. 1988. Rancidity and its measurement in edible oils and snackfoods. Analyst 113, 213-224. http://dx.doi.org/10.1039/an9881300213

Sánchez Y, Díaz M, Hernández F, Gil D, García G. 2011. Antioxidant effects of an ozonized teobroma oil formulation on damaged-inflammatory rat skin. Grasas Aceites 62, 105-110, 2011. http://dx.doi.org/10.3989/gya.055710

Sechi LA, Lezcano I, Nuñez N, Espim M, Dupre I, Pinna A, Molicotti P, Zanetti S. 2001. Antibacterial activity of ozonized sunflower oil (Oleozon). J. Appl. Microbiol. 90, 279-284. http://dx.doi.org/10.1046/j.1365-2672.2001.01235.x PMid:11168731

Statistic. 2003. Release, 6 Copyright Statsoft. Inc: All rights reserved.

Viebahn R. 1993. Therapeutical efficacy and toxicity of ozone induced peroxides. Proceeding of the 11th Ozone World Congress. San Francisco. USA. 133-144.




Copyright (c) 2012 Consejo Superior de Investigaciones Científicas (CSIC)

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.


Contact us grasasyaceites@ig.csic.es

Technical support soporte.tecnico.revistas@csic.es