Grasas y Aceites, Vol 64, No 1 (2013)

Separation of cis/trans fatty acid isomers on gas chromatography compared to the Ag-TLC method


https://doi.org/10.3989/gya.073312

C. Ravi Kiran
National Institute for Interdisciplinary Science and Technology (formerly RRL), Council of Scientific and Industrial Research, India

M. V. Reshma
National Institute for Interdisciplinary Science and Technology (formerly RRL), Council of Scientific and Industrial Research, India

A. Sundaresan
National Institute for Interdisciplinary Science and Technology (formerly RRL), Council of Scientific and Industrial Research, India

Abstract


The present study investigates the separation of the cis/trans isomers of fatty acids on the 75 m SP2560 column under different gas chromatographic (GC) conditions including an isothermal program and a time-temperature program. The time-temperature program showed improved separation of cis/trans isomers of C14:1, C16:1, C18:1, C18:2 and C18:3 fatty acids along with short chain fatty acids compared to the isothermal program. The separation of trans/trans isomers of C18:1 fatty acids including elaidic acid (C18:1 ∆9t) and vaccenic acid (C18:1 ∆11t) was difficult with the time-temperature program. The thin layer chromatography impregnated with silver nitrate (Ag- TLC) method was performed to separate cis/trans fractions and GC analysis was carried out with the trans fraction. But GC analysis showed a co-elution of trans isomers of C18:1 fatty acid. Thus the study shows that a time-temperature programmed GC method with the highly polar cyanopropyl column is sufficient to resolve trans fatty acids along with short chain fatty acids when a large number of samples has to be analyzed.

Keywords


Ag-TLC fractionation; Gas chromatography; Resolution; Trans fatty acids

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