Grasas y Aceites, Vol 64, No 5 (2013)

Natural antioxidants of the Jaffna variety of Moringa Oleifera seed oil of Indian origin as compared to other vegetable oils

A. S. Bhatnagar
Department of Lipid Science & Traditional Foods. CSIR-Central Food Technological Research. Institute, India

A. G. Gopala Krishna
Department of Lipid Science & Traditional Foods. CSIR-Central Food Technological Research. Institute, India


The Moringa oleifera crop is grown all over India for its nutritious pods, leaves and seeds. M. oleifera seeds are endowed with a high oil content (~39%) which resembles olive oil because of its high oleic acid content (~65-75%). The objective of the present study was to evaluate the natural antioxidants of crude seed kernel oil from the Jaffna variety of M. oleifera (MSO). Natural antioxidants like tocopherols and phenolics as well as minor compounds such as sterols and carotenoids present in MSO and its radical scavenging activity were measured in comparison to dried coconut kernel (copra) crude oil (CNO), crude sesame oil (SESO), crude niger seed oil (NSO), crude palm oil (CPO), crude rice bran oil (RBO) and commercially refined groundnut oil (GNO). MSO contained 88 ppm of total tocopherols with α- tocopherol (56.2 ppm) being the major tocopherol. MSO also contained 117.9 ppm of total phenolics with gallic acid (48.5 ppm) being the major phenolic. MSO contained fair amounts of sterols (1700.8 ppm) and carotenoids (17.9 ppm). MSO showed an IC50 value of 35.5 mg mL–1. This study indicates that MSO has antioxidant characteristics which are better than CNO and comparable to those of GNO.


IC50 value; Indian seed oil; Moringa oleifera Jaffna variety; Phenolics; Radical scavenging activity; Tocopherols; Total carotenoids; Total sterols.

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Abdulkarim SM, Long K, Lai OM, Muhammad SKS, Ghazali HM. 2005. Some physico-chemical properties of Moringa oleifera seed oil extracted using solvent and aqueous enzymatic methods. Food Chem. 93, 253-263.

Anwar F, Syeda NZ, Umer R. 2006. Characterization of Moringa oleifera seed oil from drought and irrigated regions of Punjab, Pakistan. Grasas Aceites 57, 160-168.

Anwar F, Latif S, Ashraf M, Gilani AH. 2007. Moringa oleifera: a food plant with multiple bio-chemical and medicinal uses- a review. Phytother. Res. 21, 17-25. PMid:17089328

Bhatnagar AS, Hemavathy J, Gopalakrishna AG. 2013. Development of a rapid method for estimation of lignans content in sesame oil. J. Food. Sci. Technol.

Bhatnagar AS, Prasanthkumar PK, Hemavathy J, Gopalakrishna AG. 2009. Fatty acid composition, oxidative stability and radical scavenging activity of vegetable oil blends with coconut oil. J. Am. Oil Chem. Soc. 86, 991-999.

Christie WW. 2012. The Lipid Library. (Accessed on 20th March 2012).

Dauqan E, Sani HA, Abdullah A, Muhamad H, Top AGM. 2011. Vitamin E and beta carotene composition in four different vegetable oils. Am. J. App. Sci. 8, 407-412.

Fahey JW. 2005. Moringa oleifera: A Review of the Medical Evidence for Its Nutritional, Therapeutic, and Prophylactic Properties. Part1. Trees for Life J. 1, 5-20.

Firestone D. 1998. AOCS method No. Ca 6a-40, Ce 8-89. Official methods and recommended practices of the American Oil Chemists Society, 5th Ed. AOCS press, 1608, Broadmoore drive Champaign, Illinois-61826 USA.

Gopala Krishna AG, Khatoon S, Shiela PM, Sarmandal CV, Indira TN, Mishra A. 2001. Effect of refining of crude rice bran oil on the retention of oryzanol in the refined oil. J. Am. Oil Chem. Soc. 78, 127-131.

Graphpad instat demo-[DATASET1.ISD]. 2012. Graphpad Software Inc. 11452, EI Camino Real, #215, San Diego, 92130 USA.

Kamal-Eldin A, Appelqvist LA. 1994. Variations in the composition of sterols, tocopherols and lignans in seed oils from four sesamum species. J. Am. Oil Chem. Soc. 71, 149-156.

Kamal-Eldin A, Andersson R. 1997. A multivariate study of the correlation between tocopherol content and fatty acid composition in vegetable oils. J. Am. Oil Chem. Soc. 74, 375-380.

Lalas S, Tsaknis J. 2002. Characterization of Moringa oleifera seed oil variety Periyakulam-1. J. Food Comp. Anal. 15, 65-77.

Latif S, Anwar F. 2008. Quality assessment of Moringa concanensis seed oil extracted through solvent and aqueous-enzymatic techniques. Grasas Aceites 59, 69-75.

Manzoor M, Anwar F, Iqbal T, Bhanger MI. 2007. Physicochemical characterization of Moringa concanensis seeds and seed oil. J. Am. Oil Chem. Soc. 84, 413-419.

Namiki M. 2007. Nutraceutical functions of sesame: A Review. Cri. Rev. Food Sci. Nutr. 47, 651-673. PMid:17943496

Ogunsina BS, Indira TN, Bhatnagar AS, Radha C, Debnath S, Gopala Krishna AG. 2011. Quality characteristics and stability of Moringa oleifera seed oil of Indian origin. J. Food Sci. Technol.

Paquot C, Havtfenne A (eds). 1987. IUPAC method No. 2.301. Standard methods for analysis of oils, fats and derivatives. International Union of Pure and Applied Chemistry, 7th edn. Blackwell, Oxford, pp. 174-182.

Rahman IMM, Barua S, Nazimuddin M, Begum ZA, Rahman MA, Hasegawa H. 2009. Physicochemical properties of Moringa oleifera Lam. seed oil of the indigenous-cultivar of Bangladesh. J. Food Lipids. 16, 540-553.

Ramachadran C, Peter KV, Gopalakrishnan PK. 1980. Drumstick (Moringa oleifera): A multipurpose Indian vegetable. Econ. Bot. 34, 276-283.

Sabir SM, Hayat I, Gardezi SDA. 2003. Estimation of sterols in edible fats and oils. Pak. J. Nutr. 2, 178-181.

Sengupta A, Gupta MP. 1970. Studies on seed fat composition of Moringaceae family. Fette Seifen Anstrichm. 72, 6-10.

Siger A, Nogala-Kalucka M, Lampart-Szczapa E. 2008. The content and antioxidant activity of phenolic compounds in cold-pressed plant oils. J. Food Lipids. 15, 137-149.

Tsaknis J, Spiliotis V, Lalas S, Gergis V, Dourtoglou V. 1999. Quality changes of Moringa oleifera, variety Mbololo of Kenya, seed oil during frying. Grasas Aceites. 50, 37-48.

Taga MS, Miller EE, Pratt DE. 1984. Chia seeds as a source of natural lipid antioxidants. J. Am. Oil Chem. Soc. 61, 928-993.

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