Grasas y Aceites, Vol 65, No 2 (2014)

Production, characterization and anticancer activity of Candida bombicola sophorolipids by means of solid state fermentation of sunflower oil cake and soybean oil

M. M. Rashad
Biochemistry Department, Division of Genetic Engineering and Biotechnology, National Research Center, Egypt

M. U. Nooman
Biochemistry Department, Division of Genetic Engineering and Biotechnology, National Research Center, Egypt

M. M. Ali
Biochemistry Department, Division of Genetic Engineering and Biotechnology, National Research Center, Egypt

A. S. Al-kashef
Biochemistry Department, Division of Genetic Engineering and Biotechnology, National Research Center, Egypt

A. E. Mahmoud
Biochemistry Department, Division of Genetic Engineering and Biotechnology, National Research Center, Egypt


The production of sophorolipids by Candida bombicola NRRL Y- 17069 grown in a mixture of sunflower oil cake and crude soybean oil as economic substrates with different fermentation techniques was studied. The highest yield (49.5 g·100 g−1 substrates) was obtained from solid state fermentation after employing a new concept for extraction by methanol (E I) followed by ethyl acetate (E II), then partially purified with hexane (E III). The course of time of fermentation was also studied, and E I extracted of the 12th day showed the minimum surface tension (45 mN·m−1) at a critical micelle dilution (CMD) of 10% concentration. The produced sophorolipids were characterized and confirmed by FTIR and 1H NMR spectroscopy. The anticancer activity of the produced compounds was assessed against MCF-7, HepG2, A549, HCT116 cancer cell lines and the results revealed that E III and E IV (a mixture of E I & E III) act as promising anticancer agents in HepG2 and A549 by inhibiting urokinase and histone deacetylase activities.


Anticancer activity; Candida bombicola; Solid state fermentation; Sophorolipids; Soybean oil; Sunflower oilcake

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