Grasas y Aceites, Vol 66, No 2 (2015)

Determination of pesticide residues in olives by liquid extraction surface analysis followed by liquid chromatography/tandem mass spectrometry

M. C. Gómez-Almenar
IFAPA Centro Venta del Llano, Spain

J. A. García-Mesa
IFAPA Centro Venta del Llano, Spain


Nowadays, pesticides are essential in modern agriculture for crop protection, however, this use supposes a potential risk for human health and the environment. Traditional techniques of pesticide determination require the use of laborious and complex extraction methods to separate pesticides from the matrix, above all in fatty matrices like olives. For this reason, a new simple, rapid, cheap and selective method for the extraction and quantification of the most frequently used pesticides in olive growing has been developed. Pesticide determination was carried out by ultra-performance liquid chromatography (UPLC) coupled with triple-quadrupole tandem mass spectrometry (MS/MS). Mean recoveries were found in a range between 73 and 114% with relative standard deviations lower than 20% in most pesticides evaluated and the limits of detection (LODs) and quantification (LOQs) were lower than 4 μg· kg-1 and 8 μg· kg-1, respectively. Finally, this method was applied to the analysis of 25 olive samples where Dimethoate and Terbuthylazine were detected in some cases, but their results were lower than 15 μg· kg-1.


Food analysis; Liquid extraction; Olives; Pesticide residues; UPLC-MS/MS

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