Essential oils from Egyptian aromatic plants as antioxidant and novel anticancer agents in human cancer cell lines

Authors

  • M. M. Ramadan Chemistry of Flavour and Aroma Department National Research Centre
  • M. M. Ali Biochemistry Department, National Research Centre
  • K. Z. Ghanem Food Science and Nutrition Department, National Research Centre - Clinical Nutrition Department, Faculty of Applied Medical Science, Jazan University
  • A. H. El-Ghorabe Chemistry of Flavour and Aroma Department National Research Centre - Chemistry Department, Faculty of Science, Al-GohfUniversity

DOI:

https://doi.org/10.3989/gya.0955142

Keywords:

Anticancer, Antioxidant, Clove Juniper, Thyme

Abstract


Inhibitors of tumor growth using extracts from aromatic plants are rapidly emerging as important new drug candidates for cancer therapy. The cytotoxicity and in vitro anticancer evaluation of the essential oils from thyme, juniper and clove has been assessed against five different human cancer cell lines (liver HepG2, breast MCF-7, prostate PC3, colon HCT116 and lung A549). A GC/MS analysis revealed that α-pinene, thymol and eugenol are the major components of Egyptian juniper, thyme and clove oils with concentrations of 31.19%, 79.15% and 82.71%, respectively. Strong antioxidant profiles of all the oils are revealed in vitro by DPPH and β-carotene bleaching assays. The results showed that clove oil was similarly potent to the reference drug, doxorubicin in prostate, colon and lung cell lines. Thyme oil was more effective than the doxorubicin in breast and lung cell lines while juniper oil was more effective than the doxorubicin in all the tested cancer cell lines except prostate cancer. In conclusion, the essential oils from Egyptian aromatic plants can be used as good candidates for novel therapeutic strategies for cancer as they possess significant anticancer activity.

Downloads

Download data is not yet available.

References

Abd-Algader NN, El-Kamali HH, Ramadan MM, Ghanem KZ, Farrag AH. 2013. Xylopia aethiopica volatile compounds protect against panadol-induced hepatic and renal toxicity in male rats. World Appl. Sci. J. 27, 10–22.

Adams RP. 1995. Identification of essential oil components by gas chromatography/mass spectrometry; Allured Publishing: Carol Stream, IL, USA.

AitM'barek L, Ait Mouse H, Jaâfari A, Aboufatima R, Benharref A, Bénard J, El Abbadi N, Bensalah M, Gamouh A, Chait A, Dalal A. 2007. Cytotoxic effect of essential oil of thyme (Thymus broussonettii) on the IGR-OV1 tumor cells resistant to chemotherapy. Braz. J. Med. Res. 40, 1537–1544. http://dx.doi.org/10.1590/S0100-879X2007001100014

Ali MM, Sohair AH. 2007. Role of some newly synthesized tetrahydro-naphthalin-thiazol derivatives as anticancer complexes. Int. J. Cancer Res. 3, 103–110. http://dx.doi.org/10.3923/ijcr.2007.103.110

Aydin E, Tu.rkez H, Geyikog˘lu F. 2013. Antioxidative, anticancer and genotoxic properties of £-pinene on N2a neuroblastoma cells. J. Biol. 68, 1004–1009.

Brusselmans K, Vrolix R, Verhoeven G, Swinnen J. 2005. Induction of cancer cell apoptosis by flavonoids is associated with their ability to inhibit fatty acid synthase activity. J. Biol. Chem. 280, 5636–5645. http://dx.doi.org/10.1074/jbc.M408177200 PMid:15533929

Chen D, Daniel KG, Chen MS, Kuhn DJ, Landis-Piwowar KR, Dou QP. 2005. Dietary flavonoids as proteasome inhibitors and apoptosis inducers in human leukemia cells. Biochem. Pharmacol. 69, 1421–1432. http://dx.doi.org/10.1016/j.bcp.2005.02.022 PMid:15857606

Dugo P, Mondello L, Dugo L, Stancanelli R, Dugo G. 2000. LC–MS for the identification of oxygen heterocyclic compounds in citrus essential oils. J. Pharmaceut. Biomed. Anal. 24, 147–154. http://dx.doi.org/10.1016/S0731-7085(00)00400-3

Elattar I. 2003. National Cancer Institute Egypt. Magnitude of liver cancer in Egypt.

Ghomi JS, Ebrahimabadi AH, Bidgoli ZD, Batooli H. 2009. GC/MS analysis and in vitro antioxidant activity of essential oil and methanol extracts of Thymus caramanicus Jalas and its main constituent carvacrol. Food Chem. 115, 1524–1528. http://dx.doi.org/10.1016/j.foodchem.2009.01.051

Gülcin I, Elmastas M, Aboul-Enein HY. 2007. Determination of antioxidant and radical scavenging activity of basil (Ocimum basilicum) assayed by different methodologies. Phytother Res. 21, 354–361. http://dx.doi.org/10.1002/ptr.2069 PMid:17221941

Gülcin I, Elmastas M, Aboul-Enein HY. 2012. Antioxidant activity of clove oil a powerful antioxidant source. Arab J Chem. 5, 489–499. http://dx.doi.org/10.1016/j.arabjc.2010.09.016

Gülcin I. 2006. Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicol. 217, 213–220. http://dx.doi.org/10.1016/j.tox.2005.09.011 PMid:16243424

Hamedo HA, Abdelmigid HM. 2009. Use of antimicrobial and genotoxicity potentiality for evaluation of essential oils as food preservatives. Open Biotechnol. J. 3, 50–56. http://dx.doi.org/10.2174/1874070700903010050

Jo JU, Park S, Parki Y, Chae YZ, Lee GH, Park GY, Jang BC. 2012. Pinus densiflora leaf essential oil induces apoptosis via ROS generation and activation of caspases in YD-8 human oral cancer cells. Inter. J. Oncol. 40, 1238–1245. PMid:22086183 PMCid:PMC3584576

Jordan MJ, Maria VL, Rota C, Loran S, Sotomayor JA. 2013. Effect of bioclimatic area on the essential oil composition and antibacterial activity of Rosmarinus officinalis L. Food Control 30, 463–468. http://dx.doi.org/10.1016/j.foodcont.2012.07.029

Lamaty G, Menut C, Bessiere JM, Zollo JM, Fekam PHA. 1987. Aromatic plants of tropical Central Africa: I. Volatile components of two annonaceae from Cameroon: Xylopia aethiopica (Dunal) A. Richard and Monodora myristica (Gaerth.) Dunal. Flavour. Frag. J. 2, 91–94. http://dx.doi.org/10.1002/ffj.2730020302

Lee KG, Shibamoto T. 2001. Antioxidant property of aroma extract isolated from clove buds Syzygium aromaticum. Food Chem. 74, 443–448. http://dx.doi.org/10.1016/S0308-8146(01)00161-3

Lesjak MM, Beara IN, Orcic DZ, Anackov GT, Balog KJ, Franci‰kovic MM. 2011. Juniperus sibirica Burgs dorf. as a novel source of antioxidant and anti-inflammatory agents. Food Chem. 124, 850–856. http://dx.doi.org/10.1016/j.foodchem.2010.07.006

Matsuo AL, Figueiredo CR, Arruda DC, Pereira FV, Scutti JAB, Massaoka MH, Travassos LR, Sartorelli P, Lago JHG. 2011. £-Pinene isolated from Schinus terebinthifolius Raddi (Anacardiaceae) induces apoptosis and confers antimetastatic protection in a melanoma model. Biochem. Biophys. Res. Commun. 411, 449–454. http://dx.doi.org/10.1016/j.bbrc.2011.06.176 PMid:21756878

Nikoli M, Glamo J, Isabel C, Ferreira FR, Calhelha RC, Fernandes Â, Markovi T, Markovi D, Giweli A, Sokovi M. 2014. Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils. Ind. Crop Prod. 52, 183–190. http://dx.doi.org/10.1016/j.indcrop.2013.10.006

Orav J, Koel M, Kailas T, Mu.u.rise M. 2010. Comparative analysis of the composition of essential oils and supercritical carbon dioxide extracts from the berries and needles of Estonian juniper (Juniperus communis L.). Procedia Chem. 2, 161–167. http://dx.doi.org/10.1016/j.proche.2009.12.023

Paul S, De Castro AJ, Lee HJ, Smolarek AK, So JY, Simi B, Wang CX, Zhou R, Rimando AM, Suh N. 2010. Dietary intake of pterostilbene, a constituent of blue berries, inhibits the b-catenin/p65 downstream signaling pathway and colon carcinogenesis in rats. Carcinogenesis 31, 1272–1278. http://dx.doi.org/10.1093/carcin/bgq004 PMid:20061362 PMCid:PMC2899944

Pepeljnjak S, Kosalec I, Kalodera Z, Blazevic N. 2005. Antimicrobial activity of juniper berry essential oil (Juniperus communis L., Cupressaceae). ActaPharmaceut. 55, 417–422.

Pisano M, Pagnan G, Loi M, Mura ME, Tilocca MG, Palmieri G, Fabbri D, Dettori MA, Delogu G. 2007. Antiproliferative and pro-apoptotic activity of eugenol-related biphenyls on malignant melanoma cells. Mol. Cancer 8, 234–242.

Prashar A, Locke IC, Evans CS. 2006. Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cells. Cell Proliferat. 39, 241–248. http://dx.doi.org/10.1111/j.1365-2184.2006.00384.x PMid:16872360

Ramadan MM, Abd-Algader NN, El-kamali HH, Ghanem KZ, Farrag AH. 2013a. Volatile compounds and antioxidant activity of the aromatic herb Anethum graveolens. J. Arab Soc. Medical Res. 8, 79–88. http://dx.doi.org/10.4103/1687-4293.123791

Ramadan MM, Abd Algader NN, El-kamali HH, Ghanem KZ, Farrag AH. 2013b. Chemo preventive effect of Coriandrum sativum fruits on hepatic toxicity in male rats. World J. Med. Sci. 8, 322–333.

Ramadan MM, Yehia HA, Shaheen MS, Abed EL, Fattah MS. 2014. Aroma volatiles, antibacterial, antifungal and antioxidant properties of essential oils obtained from some spices widely consumed in Egypt. American-Eurasian J. Agric. Environ. Sci. 14, 486–494.

Rosato A, Vitali C, Gallo D, Balenrano L, Mallamaci R. 2008. The inhibition of Candida albicans by selected essential oils and their synergism with amphotericin-B. Phytomed. 15, 635–638. http://dx.doi.org/10.1016/j.phymed.2008.05.001 PMid:18579358

Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR. 1990. New colorimetric cytotoxicity assay for anticancerdrug screening. J. Nat. Cancer Instit. 82, 1107–1112. http://dx.doi.org/10.1093/jnci/82.13.1107 PMid:2359136

Teixeira B, Marques A, Ramos C, Neng NR, Nogueir JMF, Saraiva JA, Nunes ML. 2013. Chemical composition and antibacterial and antioxidant properties of commercial essential oils. Ind. Crop Prod. 43, 587–595. http://dx.doi.org/10.1016/j.indcrop.2012.07.069

Tsukamoto Y, Fukutani S, Takeuchi S, Okamoto T, Mori M. 1989. Some phenolic compounds stimulate the proliferation of human pulpal fibroblasts. Shika Kiso Igakkai Zasshi 31, 357–362. http://dx.doi.org/10.2330/joralbiosci1965.31.357 PMid:2519277

Wang W, Li N, Luo M, Zu Y, Efferth T. 2012. Antibacterial activity and anticancer activity of Rosmarinus officinalis L. essential oil compared to that of its main components. Molecules 17, 2704–2713. http://dx.doi.org/10.3390/molecules17032704 PMid:22391603

Published

2015-06-30

How to Cite

1.
Ramadan MM, Ali MM, Ghanem KZ, El-Ghorabe AH. Essential oils from Egyptian aromatic plants as antioxidant and novel anticancer agents in human cancer cell lines. Grasas aceites [Internet]. 2015Jun.30 [cited 2024Mar.29];66(2):e080. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1545

Issue

Section

Research