Grasas y Aceites, Vol 66, No 4 (2015)

Glycerolysis of sardine oil catalyzed by a water dependent lipase in different tert-alcohols as reaction medium


https://doi.org/10.3989/gya.0238151

Á. G. Solaesa
Department of Biotechnology and Food Science (Chemical Engineering Section), University of Burgos, Spain

M. T. Sanz
Department of Biotechnology and Food Science (Chemical Engineering Section), University of Burgos, Spain

R. Melgosa
Department of Biotechnology and Food Science (Chemical Engineering Section), University of Burgos, Spain

S. L. Bucio
Department of Biotechnology and Food Science (Chemical Engineering Section), University of Burgos, Spain

S. Beltrán
Department of Biotechnology and Food Science (Chemical Engineering Section), University of Burgos, Spain

Abstract


The production of monoacylglycerol rich in polyunsaturated fatty acids (PUFA) via enzymatic glycerolysis of sardine oil in a homogeneous system was evaluated. Reactions were conducted in two different tert-alcohols. Based on the phase equilibrium data, the amount of solvent added to create a homogeneous system has been calculated and optimized. The immobilized lipase used in this work was Lipozyme RM IM from Rhizomucor miehei, a water dependent lipase. The amount of water added as well as other reaction parameters were studied to evaluate the optimum conditions for monoacylglycerol obtencion. An initial reactant mole ratio glycerol to sardine oil 3:1, 12 wt% of water based on glycerol content and 10 wt% of lipase loading (based on weight of reactants), achieved a MAG yield of around 70%, with nearly 28 wt% PUFA, with low free fatty acid content (lower than 18 wt%).

Keywords


Fish oil; Glycerolysis; Lipase; Monoacylglycerol; Rhizomucor miehei; Tert-alcohols

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