Grasas y Aceites, Vol 66, No 4 (2015)

19F NMR method for the determination of quality of virgin olive oil

L. L. Zhou
School of Environmental and Chemical Engineering, China

C. Li
School of Environmental and Chemical Engineering, China

X. C. Weng
School of Environmental and Chemical Engineering, China

X. M. Fang
Shanghai Entry-Exit Inspection Quarantine Bureau, China

Z. H. Gu
Shanghai Food Security Officce, China


This paper reported a potential analytical technique based on NMR spectroscopy for the determination of quality of olive oil. The model compounds with active hydrogen, including free sterols, free aliphatic alcohols, phenolics, and free fatty acids were determined by 19F NMR upon derivation with 4-fluorobenzoyl chloride. Integration of the appropriate signals of the derivatives of the compounds in the corresponding 19F NMR spectrum allows for the quantification of these compounds. 37 Samples of commercial olive oil and 5 samples of other plant oils were determined by 19F NMR. The amount of diglycerides and the ratio of 1,2-diglycerides to the total amount of diglycerides were analyzed to monitor whether extra virgin olive oil was adulterated with low price olive oil and other plant oils or not. The results showed that the total diglyceride content should not be higher than 2.5% and the ratio (D) of 1,2-diglycerides to total diglycerides should be higher than 0.35 for extra virgin olive oil. This method is an easier, simpler, safer, faster and more reliable technique for the determination of the quality of olive oil and can also be extended to monitoring the quality of ordinary edible oils.


Alcohols; Diglycerides; 4-Fluorobenzoyl chloride; 19F NMR; Quality of olive oil

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