Grasas y Aceites, Vol 67, No 1 (2016)

Effect of Spanish style processing on the phenolic compounds and antioxidant activity of Algerian green table olives

S. Mettouchi
Laboratoire de Biochimie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Algeria

R. Sacchi
Laboratory of MolecularGastronomy, Faculty di Agraria, UniversitaDegliStudi Di Napoli Federico II, Italy

Z. E.D. Ould Moussa
Laboratoire de Biochimie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Algeria

A. Paduano
Laboratory of MolecularGastronomy, Faculty di Agraria, UniversitaDegliStudi Di Napoli Federico II, Italy

M. Savarese
CRIOL, Centro Ricerche per l’Industria Olearia, Italy

A. Tamendjari
Laboratoire de Biochimie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Algeria


The study was carried out on seven Algerian olive cultivars to report the effect of Spanish style processing on individual and total phenolic compounds and the changes that occur in antioxidant capacity. The results indicate that the treatment leads to losses in phenolic contents which are cultivar dependent. Sigoise is the least affected variety (12.25%) and Azzeradj from Seddouk the most affected one (94.80%). The phenolic profile shows drastic changes after processing. Hydroxytyrosol is dominant in processed olives (14.42–545.42 mg.100 g-1) while oleuropein is the major phenolic compound in fresh olives (994.27 mg.100 g-1). As a consequence to the loss in phenolic content, substantial reductions in the antioxidant activities of the extracts are noted. They are estimated to be 13.12–92.75% in scavenging activity against the DPPH radical, 37.78–93.98% in reducing capacity, 59.45–97.94% in the hydrogen peroxide radical and 7.26–51.66% in the inhibition bleaching of β-carotene. Among the processed varieties, only Sigoise presented a positive value of RACI (relative antioxidant capacity index).


Antioxidant activity; Phenolic compounds; RACI; Spanish style processing; Table olives

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Amiot MJ, Friet A, Macheix J. 1986. Importance and evolution of phenolic compounds in olive during growth and maturation. J. Agric. Food Chem. 34, 823–826.

Arslan D. 2012. Physico-chemical characteristics of olive fruits of Turkish varieties from the province of Hatay. Grasas Aceites 63, 158–166.

Arslan D, Schreiner M. 2012. Chemical characteristics and antioxidant activity of olive oils from Turkish varieties grown in Hatay province. Sci. Hortic. 144, 141–152.

Arslan D, Özcan MM. 2011. Phenolic profile and antioxidant activity of olive fruits of the Turkish variety "Sarıulak" from different locations. Grasas Aceites 62, 453–461.

Ben Othman N, Roblain D, Chammen N, Thonart P, Hamdi, M. 2009. Antioxidant phenolic compounds loss during the fermentation of Ch.toui olives. Food Chem. 116, 662–669.

Bianchi G. 2003. Lipids and phenols in table olives. Eur. J. Lipid Sci. Technol. 105, 229–242.

Blekas G, Vassilakis C, Harizanis C, Tsimidou M, Boskou D-G. 2002. Biophenols in table olives. J. Agric. Food Chem. 50, 3688–3692. PMid:12059143

Borzillo A, Iannotta N, Uccella N. 2000. Oinotria table olives: quality evaluation during ripening and processing by biomolecular components. Eur. Food Res. and Technol. 212, 113–121.

Boskou G, Fotini N, Salta Chrysostomou S, Mylona A, Chiou A, Andrikopoulos NK. 2006. Antioxidant capacity and phenolic profile of table olives from the Greek market. Food Chem. 94, 558–564.

Brenes M, Hidalgo JH, Garcia A, Rios JJ, Garcia P, Zamora R. 2000. Pinoresinol and 1-acetoxypinoresinol, two new phenolic compounds identified in olive oil. J. Am. Oil Chem. Soc. 77, 715–720.

Brenes M, de Castro A. 1998. Transformation of oleuropein and its hydrolysis products during Spanish-style green olive processing. J. Sci. Food Agric. 77, 353–358.<353::AID-JSFA50>3.0.CO;2-G

Cicerale S, Lucas L, Keast R. 2010. Biological Activities of Phenolic Compounds Present in Virgin Olive Oil. Int. J. Mol. Sci. 11, 458–479. PMid:20386648 PMCid:PMC2852848

Dourtoglou VG, Mamalos A, Makris, DP. 2006. Storage of olives (Oleaeuropaea) under CO2 atmosphere: Effect on anthocyanins, phenolics, sensory attributes and in vitro antioxidant properties. Food Chem. 99, 342–349.

El Khaloui M, Nouri A. 2007. Proc.d. d'.laboration des olives de table . base des vari.t.s Picholine Marocaine et Dahbia. Transfert Technol. Agric., 152, 1–4.

Han RM, Zhang JP, Skibsted LH. 2012. Reaction Dynamics of Flavonoids and Carotenoids as Antioxidants. Molecules 17, 2140–2160. PMid:22354191

Hayes JE, Allen P, Brunton N, O'Grady MN, Kerry JP. 2011. Phenolic composition and in vitro antioxidant capacity of four commercial phytochemical products: Olive leaf extract (Oleaeuropaea L.), lutein, sesamol and ellagic acid. Food Chem. 126, 948–955.

Hemalatha A, Girija K, Parthiban C, Saranya C, Anantharaman P. 2013. Antioxidant properties and total phenolic content of a marine diatom, Naviculaclavata and green microalgae, Chlorella marina and Dunaliella salina. Adv. App. Sci. Res. 4, 151–157.

Ilias F, Kholkhal W, Gaouar N, Bekhechi C, Bekkara FA. 2011. Antioxidant potential of olive (Oleaeuropaea L.) from Algeria. J. Nat. Prod Plant Resour. 1, 29–35.

IOC (International Olive Council). 2013. Market Newsletter, No 76 – Octobre 2013, production d'olives de table, 1–6.

Kia H, Hafidi A. 2014. Chemical composition changes in four green olive cultivars during spontaneous fermentation. LWT-Food Sci. Technol. 57, 663–670.

Malheiro R, Sousa A, Casal S, Bento A, Pereira JA. 2011. Cultivar effect on the phenolic composition and antioxidant potential of stoned table olives. Food Chem. Toxicol. 49, 450–457. PMid:21108983

Marsilio V, d'Andria R, Lanza B, Russi F, Iannucci E, Lavini A. 2006. Effect of irrigation and lactic acid bacteria inoculants on the phenolic fraction, fermentation and sensory characteristics of olive (Oleaeuropaea L. cv. Ascolanatenera) fruits. J. Sci. Food Agric. 86, 1005–1013.

McDonald S, Prenzler PD, Antolovich M, Robards K. 2001. Phenolic content and antioxidant activity of olive extracts. Food Chem. 73, 73–84.

Nadour M, Michaud P, Moulti-Mati F. 2012. Antioxidant Activities of Polyphenols Extracted from Olive (Oleaeuropaea) of Chemlal Variety. Appl. Biochem. Biotechnol. 167, 1802–1810. PMid:22402836

Nsimba RY, Kikuzaki H, Konishi Y. 2008. Antioxidant activity of various extracts and fractions of Chenopodium quinoa and Amaranthus spp. Seeds. Food Chem. 106, 760–766.

Pasqualone A, Nasti R, Montemurro C, Gomes T. 2014. Effect of natural style processing on the oxidative and the lipid fraction of d hydrolytic degradation of table olives. Food Control. 37, 99–103.

Pereira J-A, Pereira A-PG, Ferreira ICFR, Valenta P, Andrade PB, Seabra R, Estevinho L, Bento A. 2006. Table Olives from Portugal: Phenolic Compounds, Antioxidant Potential and Antimicrobial Activity. J. Agric. Food Chem. 54, 8425–8431. PMid:17061816

Piscopo A, De Bruno A, Zappia A, Poiana M. 2014. Antioxidant activity of dried green olives (Caroleacv.). LWT-Food Sci. Technol. 58, 49–54.

Rodríguez H, Curiel JA, Landete JM, de las Rivas B, de Felipe FL, Gómez-Cordovés, C, Manchego JM, Mu-oz R. 2009. Food phenolics and lactic acid bacteria. Int. J. Food Microbiol. 132, 79–90. PMid:19419788

Romero C, Brenes M, Yousfi K, Garcia P, Garc.a A, Garrido A. 2004. Effect of cultivar and processing method on the contents of polyphenols in table olives. J. Agric. Food Chem. 52 (3), 479–484. PMid:14759136

Rovellini P, Cortesi N. 2002. Liquid chromatography-mass spectrometry in the study of oleuropein and ligstroside aglycons in virgin olive oil: aldehydic, dialdehydic forms and their oxidized products. Riv. Ital. Sostanze Gr. 79, 1–14.

Sahan Y, Cansev A, Gulen H. 2013. Effect of Processing Techniques on Antioxidative Enzyme Activities, Antioxidant Capacity, Phenolic Compounds, and Fatty Acids of Table Olives. Food Sci. Biotechnol. 22, 613–620.

Savarese M, De Marco E, Sacchi R. 2007. Characterization of phenolic extracts from olives (Oleaeuropaea cv Pisciottana) by electrospray ionization mass spectrometry. Food Chem. 105, 761–770.

Soni MG, Burdock GA, Christian M-S, Bitler C-M, Crea R. 2006. Safety assessment of aqueous olive pulp extract as an antioxidant or antimicrobial agent in foods. Food Chem. Toxicol. 44, 903–915. PMid:16530907

Sousa A, Ferreira ICFR, Barros L, Bento A, Pereira J-A. 2008. Effect of solvent and extraction temperatures on the antioxidant potential of traditional stoned table olives ''alcaparras''. Lebensm. Wiss. Technol. 41, 739–745.

Sroka Z, Cisowski W. 2003. Hydrogen peroxide scavenging, antioxidant and anti-radical activity of some phenolic acids. Food Chem. Toxicol. 41, 753–758.

Sun T, Tanumihardjo SA. 2007. An Integrated Approach to Evaluate Food Antioxidant Capacity. J. Food Sci. 72, 159–165. PMid:18034745

Velkov ZA, Kolev MK, Tadjer AV. 2007. Modeling and statistical analysis of DPPH Scavenging activity of phenolics. Collect. Czech. Chem. Commun. 72, 1461–1471.

Vinha AF, Ferreres F, Silva BM, Valent.o P, Gon.alves A, Pereira JA, Oliveira M-B, Seabra R-M, Andrade PB. 2005. Phenolic profiles of Portuguese olive fruits (Oleaeuropaea L.). Influences of cultivar and geographical origin. Food Chem. 89, 561–568.

Zhan Y, Hong Dong C, Yao Y-J. 2006. Antioxidant activities of aqueous extract from cultivated fruit- bodies of Cordycepsmilitaris (L.) Link in vitro. J. Integr. Plant. Biol. 48, 1365–1370.

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