Evaluation of diffusional effects on kinetics of soybean lecithin hydrolysis using A2 phospholipase
DOI:
https://doi.org/10.3989/gya.2004.v55.i2.160Keywords:
Diffusional effects, Effectiveness factor, Immobilized A2 phospholipase, Mass transport, Soybean lecithinAbstract
Soluble or immobilized A2 phospholipase liberates one mole of fatty acid of the C-2 position in soybean lecithin phospholipids to product lysolecithins. When using the enzyme in its immobilized form the enzymatic activity decreases due to substrate and product transport phenomenon. By comparison of the kinetic parameters of the hydrolysis reaction catalized by A2 phospholipase immobilized on alumina or on DEAE-Sephadex against those of the soluble enzyme, for soybean lecithin hydrolysis reaction, the diffusive effects were evaluated. The decrease of the reaction rate was quantified in presence of diffusive effects and the effectiveness factors were calculated. The Michaelis-Menten kinetic parameters were determined: KM = 8,2.10-2 mol·l-1 and 5,36.10-2 mol·l-1 and Vmax = 7,7.10-5 mol·l-1 .min-1 and 9,5.10-5 mol·l-1 ·min-1 for the enzyme immobilized on alumina and on DEAE-Sephadex, respectively. The effectiveness factors were 0,277 for alumina and 0,376 for DEAE-Sephadex.
Downloads
Download data is not yet available.
Downloads
Published
2004-06-30
How to Cite
1.
Maroto B, Camusso C, Zaritzky N. Evaluation of diffusional effects on kinetics of soybean lecithin hydrolysis using A2 phospholipase. Grasas aceites [Internet]. 2004Jun.30 [cited 2024Apr.25];55(2):148-54. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/160
Issue
Section
Research
License
Copyright (c) 2004 Consejo Superior de Investigaciones Científicas (CSIC)
This work is licensed under a Creative Commons Attribution 4.0 International License.
© CSIC. Manuscripts published in both the printed and online versions of this Journal are the property of Consejo Superior de Investigaciones Científicas, and quoting this source is a requirement for any partial or full reproduction.All contents of this electronic edition, except where otherwise noted, are distributed under a “Creative Commons Attribution 4.0 International” (CC BY 4.0) License. You may read here the basic information and the legal text of the license. The indication of the CC BY 4.0 License must be expressly stated in this way when necessary.
Self-archiving in repositories, personal webpages or similar, of any version other than the published by the Editor, is not allowed.