Grasas y Aceites, Vol 68, No 1 (2017)

Improving oxidative stability of olive oil: Incorporation of Spirulina and evaluation of its synergism with citric acid

N. Alavi
Department of Food Science and Technology, School of Agriculture, Shiraz University, Iran, Islamic Republic of

M. T. Golmakani
Department of Food Science and Technology, School of Agriculture, Shiraz University, Iran, Islamic Republic of


The effects of different Spirulina concentrations used alone and in combination with citric acid on the oxidative stability of olive oil were assessed. The amounts of primary and secondary oxidation products produced in Spirulina samples were lower than that of the control. The improved oxidative stability indices of Spirulina samples with and without citric acid were in the range of 85.20–94.47% and 258.10–260.21%, respectively. In comparison with the control, Spirulina samples manifested significantly higher carotenoid and chlorophyll contents at the beginning and end of the storage period. The presence of these bioactive compounds results from the presence of Spirulina in the medium and can thus retard the oxidation of olive oil. A higher oxidative stability was reached using BHT in comparison with Spirulina samples. Furthermore, no synergistic action was observed in possible connections between citric acid and Spirulina. In conclusion, Spirulina can enhance oxidative stability and improve the shelf life of olive oil.


Arthrospira platensis; Citric acid; Natural antioxidant; Olive oil; Spirulina; Synergistic effect

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Antolovich M, Prenzler PD, Patsalides E, Mcdonald S, Robards K. 2002. Methods for testing antioxidant activity. Analyst. 127, 183–198.

AOAC 1997. Official methods of analysis of AOAC international. Association of Official Analytical Chemists.Washington, DC (USA).

AOCS 1998. Official Methods and Recommended Practices of the American Oil Chemists' Society. Illinois (US), AOCS Press.

Apak R, Güçlü K, Özyürek M, Karademir SE. 2004. Novel Total Antioxidant Capacity Index for Dietary Polyphenols and Vitamins C and E, Using Their Cupric Ion Reducing Capability in the Presence of Neocuproine: CUPRAC Method. J. Agric. Food Chem. 52, 7970–7981. PMid:15612784

Barjol J-L.2013. Introduction, In Aparicio R, Harwood J. (Eds.) Handbook of Olive Oil: Analysis and Properties. Springer, 4, 6, 12. PMid:23483508

Bermejo P, Pi-ero E, Villar ÁM. 2008. Iron-chelating ability and antioxidant properties of phycocyanin isolated from a protean extract of Spirulina platensis. Food Chem. 110, 436– 445. PMid:26049237

Cervejeira Bolanho B, Buranelo Egea M, Morocho Jacome AL, Campos I, Monteiro De Carvalho JC, Godoy Danesi ED. 2014. Antioxidant and nutritional potential of cookies enriched with Spirulina platensis and sources of fibre. J. Food Nutr. Res. (Bratislava, Slovakia) 53, 171–179.

Chakraborty K, Joseph D, Joseph D. 2016. Concentration and stabilization of C20–22 n-3 polyunsaturated fatty acid esters from the oil of Sardinella longiceps. Food Chem. 199, 828– 837. PMid:26776041

Criado MN, Romero MP, Casanovas M, Motilva MJ. 2008. Pigment profile and colour of monovarietal virgin olive oils from Arbequina cultivar obtained during two consecutive crop seasons. Food Chem. 110, 873–880. PMid:26047273

Farvin KS, Jacobsen C. 2015. Antioxidant Activity of Seaweed Extracts: In Vitro Assays, Evaluation in 5% Fish Oil-in-Water Emulsions and Characterization. J. Am. Oil Chem. Soc. 92, 571–587.

Fradique M, Batista AP, Nunes MC, Gouveia L, Bandarra NM, Raymundo A. 2010. Incorporation of Chlorella vulgaris and Spirulina maxima biomass in pasta products. Part 1: Preparation and evaluation. J. Sci. Food Agric. 90, 1656– 1664. PMid:20564448

Golmakani MT, Rezaei K, Mazidi S, Razavi SH. 2012a. ?-Linolenic acid production by Arthrospira platensis using different carbon sources. Eur. J. Lipid Sci. Technol. 114, 306–314.

Golmakani MT, Rezaei K, Mazidi S, Razavi SH. 2012b. Effect of alternative C2 carbon sources on the growth, lipid, and ?-linolenic acid production of spirulina (Arthrospira platensis). Food Sci. Biotechnol. 21, 355–363.

Gordon MH, Weng XC. 1992. Antioxidant properties of extracts from tanshen (Salvia miltiorrhiza Bunge). Food Chem. 44, 119–122.

Habibi M, Golmakani MT, Mesbahi G, Majzoobi M, Farahnaky A. 2015. Ultrasound-accelerated debittering of olive fruits. Innovative Food Sci. Emerging Technol. 31, 105–115.

Ismaiel MMS, El-Ayouty YM, Piercey-Normore MD. 2014. Antioxidants characterization in selected cyanobacteria. Ann. Microbiol. 64, 1223–1230.

Katsoyannos E, Batrinou A, Chatzilazarou A, Bratakos SM, Stamatopoulos K, Sinanoglou VJ. 2015. Quality parameters of olive oil from stoned and nonstoned Koroneiki and Megaritiki Greek olive varieties at different maturity levels. Grasas Aceites 66, e067.

Keramat M, Golmakani, M-T. 2016. Effect of Thymus vulgaris and Bunium persicum essential oils on the oxidative stability of virgin olive oil. Grasas Aceites 67, e162.

Kindleysides S, Quek SY, Miller MR. 2012. Inhibition of fish oil oxidation and the radical scavenging activity of New Zealand seaweed extracts. Food Chem. 133, 1624–1631.

Luzia MR, Trugo LC, Da Paixao KCC, Marcı?Lio R, De Maria CAB, Quinteiro LMC. 1998. Effect of 5-Caffeoylquinic Acid in the Presence of Metal Chelators on Soybean Oil Oxidative Stability. LWT-Food Sci. Technol. 31, 64–68.

Mendiola JA, Martín-Álvarez PJ, Se-OráNs FJ, Reglero G, Capodicasa A, Nazzaro F, Sada A, Cifuentes A, Ibá-ez E. 2009. Design of Natural Food Antioxidant Ingredients through a Chemometric Approach. J. Agric. Food Chem. 58, 787–792. PMid:20025217

Minguez-Mosquera MI, Rejano-Navarro L, Gandul-Rojas B, Sanchezgomez AH, Garrido-Fernandez J. 1991. Color-pigment correlation in virgin olive oil. J. Am. Oil Chem. Soc. 68, 332–336.

Pokorny, J. 2007. Antioxidants in food preservation, In Rahman MS. (Ed.) Handbook of Food Preservation. CRC press, 274–275.

Raheem A, Azlina WW, Yap YT, Danquah MK, Harun R. 2015. Thermochemical conversion of microalgal biomass for biofuel production. Renewable Sustainable Energy Rev. 49, 990–999.

Santoyo S, Herrero M, Senorans FJ, Cifuentes A, Ibá-ez E, Jaime L. 2006. Functional characterization of pressurized liquid extracts of Spirulina platensis. Eur. Food Res. Technol. 224, 75–81.

Shalaby EA, Shanab SMM. 2013. Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. Indian J. Geo-Mar. Sci. 42, 556–564.

Siriwardhana N, Lee KW, Kim SH, Ha JH, Park GT, Jeon YJ. 2004. Lipid Peroxidation Inhibitory Effects of Hizikia fusiformis Methanolic Extract on Fish Oil and Linoleic acid. Food Sci. Technol. Int. 10, 65–72.

Spolaore P, Joannis-Cassan C, Duran E, Isambert A. 2006. Commercial applications of microalgae. J. Biosci. Bioeng. 101, 87–96. PMid:16569602

?ükran D, Güne? T, Sivaci R. 1998. Spectrophotometric determination of chlorophyll-A, B and total carotenoid contents of some algae species using different solvents. Turk. J. Bot. 22, 13–18.

Taghvaei M, Jafari SM.2013. Application and stability of natural antioxidants in edible oils in order to substitute synthetic additives. J. Food Sci. Technol. 52, 1272–1282. PMid:25745196 PMCid:PMC4348291

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