Grasas y Aceites, Vol 68, No 1 (2017)

Anti-inflammatory activity of two varieties of pumpkin seed oil in an adjuvant arthritis model in rats

S. Y. Al-Okbi
Food Sciences and Nutrition Department, National Research Centre, Egypt

D. A. Mohamed
Food Sciences and Nutrition Department, National Research Centre, Egypt

E. Kandil
Biochemistry Department, Faculty of Science, Ain Shams University, Egypt

M. A. Abo-Zeid
Genetics and Cytology Department, National Research Centre, Egypt

S. E. Mohammed
Food Sciences and Nutrition Department, National Research Centre, Egypt

E. K. Ahmed
Biochemistry Department, Faculty of Science, Ain Shams University


The aim of the present research was to evaluate the anti-inflammatory activity of pumpkin seed oils (PSOs) of an Egyptian and European variety, in a rat model of adjuvant arthritis. Edema thickness, plasma tumor necrosis factor-α (TNF-α) and erythrocyte sedimentation rate (ESR) were determined as inflammatory biomarkers while malondialdehyde (MDA) and total antioxidant capacity (TAC) were assessed as indicative of oxidative stress. Chromosomal aberration, sperm shape abnormalities, and DNA fragmentations are cytogenetic parameters which aid in tracing inflammatory and oxidative activity. Phenolic contents and β-carotene were determined in PSOs. The results showed elevated ESR, plasma TNF-α, plasma MDA, liver cellular DNA fragmentation, bone marrow chromosomal aberration, sperm shape abnormalities with a reduction in plasma TAC and body weight gain in an adjuvant arthritis control compared to a healthy control. Administration of low and high doses of either Egyptian or European PSO improved all the aforementioned parameters with variable degrees.


Adjuvant arthritis; Anti-inflammatory; β-Carotene; Phenolic contents; Pumpkin seed oils

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