Grasas y Aceites, Vol 68, No 2 (2017)

Characterization of Leucaena (Leucaena leucephala) oil by direct analysis in real time (DART) ion source and gas chromatography

M. Alam
Research Center-College of Science, King Saud University, Saudi Arabia

N. M. Alandis
Department of Chemistry, College of Science, King Saud University, Saudi Arabia

E. Sharmin
Department of Pharmaceutical Chemistry, College of Pharmacy, Umm Al-Qura University, Saudi Arabia

N. Ahmad
Department of Chemistry, College of Science, King Saud University, Saudi Arabia

B. F. Alrayes
Central Laboratory, College of Science, King Saud University, Saudi Arabia

D. Ali
Department of Zoology, College of Science, King Saud University, Saudi Arabia


For the first time, we report the characterization of triacylglycerols and fatty acids in Leucaena (Leucaena leucephala) oil [LUCO], an unexplored nontraditional non-medicinal plant belonging to the family Fabaceae. LUCO was converted to fatty acid methyl esters (FAMEs). We analyzed the triacylglycerols (TAGs) of pure LUCO and their FAMEs by time-of-flight mass spectrometry (TOF-MS) followed by multivariate analysis for discrimination among the FAMEs. Our investigations for the analysis of LUCO samples represent noble features of glycerides. A new type of ion source, coupled with high-resolution TOF-MS was applied for the comprehensive analysis of triacylglycerols. The composition of fatty acid based LUCO oil was studied using Gas Chromatography (GC-FID). The major fatty acid components of LUCO oil are linoleic acid (52.08%) oleic acid (21.26%), palmitic acid (7.91%) and stearic acid (6.01%). A metal analysis in LUCO was done by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The structural elucidation and thermal stability of LUCO were studied by FT-IR, 1H NMR, 13C NMR spectroscopic techniques and TGA-DSC, respectively. We also measured the cytotoxicity of LUCO.


Cytotoxicity; Gas Chromatography; Leucaena oil; Thermal analysis; TOF-MS

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