Grasas y Aceites, Vol 68, No 2 (2017)

Characterization of Leucaena (Leucaena leucephala) oil by direct analysis in real time (DART) ion source and gas chromatography


https://doi.org/10.3989/gya.0939162

M. Alam
Research Center-College of Science, King Saud University, Saudi Arabia
orcid http://orcid.org/0000-0001-9540-8532

N. M. Alandis
Department of Chemistry, College of Science, King Saud University, Saudi Arabia
orcid http://orcid.org/0000-0002-2098-9800

E. Sharmin
Department of Pharmaceutical Chemistry, College of Pharmacy, Umm Al-Qura University, Saudi Arabia
orcid http://orcid.org/0000-0002-3262-0162

N. Ahmad
Department of Chemistry, College of Science, King Saud University, Saudi Arabia
orcid http://orcid.org/0000-0002-2913-1763

B. F. Alrayes
Central Laboratory, College of Science, King Saud University, Saudi Arabia
orcid http://orcid.org/0000-0002-5319-2785

D. Ali
Department of Zoology, College of Science, King Saud University, Saudi Arabia
orcid http://orcid.org/0000-0002-8034-4473

Abstract


For the first time, we report the characterization of triacylglycerols and fatty acids in Leucaena (Leucaena leucephala) oil [LUCO], an unexplored nontraditional non-medicinal plant belonging to the family Fabaceae. LUCO was converted to fatty acid methyl esters (FAMEs). We analyzed the triacylglycerols (TAGs) of pure LUCO and their FAMEs by time-of-flight mass spectrometry (TOF-MS) followed by multivariate analysis for discrimination among the FAMEs. Our investigations for the analysis of LUCO samples represent noble features of glycerides. A new type of ion source, coupled with high-resolution TOF-MS was applied for the comprehensive analysis of triacylglycerols. The composition of fatty acid based LUCO oil was studied using Gas Chromatography (GC-FID). The major fatty acid components of LUCO oil are linoleic acid (52.08%) oleic acid (21.26%), palmitic acid (7.91%) and stearic acid (6.01%). A metal analysis in LUCO was done by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The structural elucidation and thermal stability of LUCO were studied by FT-IR, 1H NMR, 13C NMR spectroscopic techniques and TGA-DSC, respectively. We also measured the cytotoxicity of LUCO.

Keywords


Cytotoxicity; Gas Chromatography; Leucaena oil; Thermal analysis; TOF-MS

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