Grasas y Aceites, Vol 69, No 1 (2018)

Extracellular laccase production and phenolic degradation by an olive mill wastewater isolate


https://doi.org/10.3989/gya.0776171

R. Kumar
Shamir Research Institute, University of Haifa - Department of Biological Science, Birla Institute of Technology and Science, India
orcid http://orcid.org/0000-0001-6929-9176

Y. Raizner
Shamir Research Institute, University of Haifa, Israel
orcid http://orcid.org/0000-0001-7710-5845

L. I. Kruh
Department of Biotechnology Engineering, ORT Braude College, Israel
orcid http://orcid.org/0000-0002-8342-7653

O. Menashe
Water Industry Engineering Department, Achi Racov Engineering School, Kinneret College on the Sea of Galilee, Israel
orcid http://orcid.org/0000-0001-7368-0754

H. Azaizeh
Institute of Applied Research, University of Haifa - Tel-Hai College, Department of Environmental Science, Israel
orcid http://orcid.org/0000-0002-0311-1158

S. Kapur
Department of Biological Science, Birla Institute of Technology and Science, India
orcid http://orcid.org/0000-0002-1941-9288

E. Kurzbaum
Shamir Research Institute, University of Haifa, Israel
orcid http://orcid.org/0000-0002-8258-9407

Abstract


Olive mill wastewater (OMWW) presents a challenge to the control of effluents due to the presence of a high organic load, antimicrobial agents (monomeric-polymeric phenols, volatile acids, polyalcohols, and tannins), salinity and acidity. In this study, the production of extracellular laccase, monomeric or polymeric phenol, from an OMWW isolate based on its ability to biodegrade phenols and gallic acid as a model of phenolic compounds in OMWW was investigated. Phylogenetic analysis of the 16S RNA gene sequences identified the bacterial isolate (Acinetobacter REY) as being closest to Acinetobacter pittii. This isolate exhibited a constitutive production of extracellular laccase with an activity of 1.5 and 1.3 U ml/L when supplemented with the inducers CuSO4 and CuSO4+phenols, respectively. Batch experiments containing minimal media supplemented with phenols or gallic acid as the sole carbon and energy source were performed in order to characterize their phenolic biodegradability. Acinetobacter REY was capable of biodegrading up to 200 mg/L of phenols and gallic acid both after 10 h and 72 h, respectively.

Keywords


Acinetobacter REY; Biodegradation; Extracellular laccase; Olive mill waste water; Phenolic compounds

Full Text:


HTML PDF XML

References


Ahmad SA, Shamaan NA, Arif NM, Koon GB. 2012. Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1. World J. Microbiol. Biotechnol. 28, 347–352. https://doi.org/10.1007/s11274-011-0826-z PMid:22806810

Atalla MM, Zeinab HK, Eman RH, Amani AY. 2013. Characterization and kinetic properties of the purified Trematosphaeria mangrovei laccase enzyme. Saudi J. Biol. Sci. 20, 373–381. https://doi.org/10.1016/j.sjbs.2013.04.001 PMid:24235874 PMCid:PMC3824134

Azaizeh H, Halahlih F, Najami N, Brunner D. 2012. Antioxidant activity of phenolic fractions in olive mill wastewater. Food Chem. 134, 2226–2234. https://doi.org/10.1016/j.foodchem.2012.04.035 PMid:23442678

Azaizeh H, Kurzbaum E, Said O, Jaradat H. 2015. The potential of autochthonous microbial culture encapsulation in a confined environment for phenol biodegradation. Environ. Sci. Pollut. Res. Int. 22, 15179. https://doi.org/10.1007/s11356-015-4981-x PMid:26250809

Bakhshi Z, Najafpour G, Kariminezhad E, Pishgar R. 2011. Growth kinetic models for phenol biodegradation in a batch culture of Pseudomonas putida. Environ. Technol. 32, 1835–1841. https://doi.org/10.1080/09593330.2011.562925

Baldrian P, ?najdr J. 2006. Production of ligninolytic enzymes by litter-decomposing fungi and their ability to decolorize synthetic dyes. Enzyme Microb. Technol. 39, 1023–1029. https://doi.org/10.1016/j.enzmictec.2006.02.011

Bertand B. 2013. Fungal laccases: induction and production. Rev. Mex. Ing. química 12, 473–488.

Borges A, Ferreira C, Saavedra MJ, Simoes M. 2013. Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria. Microb. Drug Resist. 19, 256–265. https://doi.org/10.1089/mdr.2012.0244 PMid:23480526

Cordova-Rosa SM, Dams RI, Cordova-Rosa EV, Radetski MR. 2009. Remediation of phenol-contaminated soil by a bacterial consortium and Acinetobacter calcoaceticus isolated from an industrial wastewater treatment plant. J. Hazard. Mater. 164, 61–66. https://doi.org/10.1016/j.jhazmat.2008.07.120

Dalfard AB, Khajeh K, Soudi MR, Naderi-Manesh H. 2006. Isolation and biochemical characterization of laccase and tyrosinase activities in a novel melanogenic soil bacterium. Enzyme Microb. Technol. 39, 1409–1416. https://doi.org/10.1016/j.enzmictec.2006.03.029

Edgar RC. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32, 1792–1797. https://doi.org/10.1093/nar/gkh340 PMid:15034147 PMCid:PMC390337

Fulzele R, Desa E, Yadav A, Shouche Y. 2011. Characterization of novel extracellular protease produced by marine bacterial isolate from the Indian Ocean. Brazilian J. Microbiol. 42, 1364–1373. https://doi.org/10.1590/S1517-83822011000400018 PMid:24031765 PMCid:PMC3768751

Ghodake G, Jadhav U, Tamboli D, Kagalkar A. 2011. Decolorization of Textile Dyes and Degradation of Mono-Azo Dye Amaranth by Acinetobacter calcoaceticus NCIM 2890. Indian J. Microbiol. 51, 501–508. https://doi.org/10.1007/s12088-011-0131-4 PMid:23024414 PMCid:PMC3209937

Iasur-Kruh L, Hadar Y, Minz D. 2011. Isolation and bioaugmentation of an estradiol-degrading bacterium and its integration into a mature biofilm. Appl. Environ. Microbiol. 77, 3734–40. https://doi.org/10.1128/AEM.00691-11 PMid:21478310 PMCid:PMC3127609

Kuddus M, Joseph B, Wasudev Ramteke P. 2013. Production of laccase from newly isolated Pseudomonas putida and its application in bioremediation of synthetic dyes and industrial effluents. Biocatal. Agric. Biotechnol. 2, 333–338. https://doi.org/10.1016/j.bcab.2013.06.002

Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33, 1870–1874. https://doi.org/10.1093/molbev/msw054 PMid:27004904

Kunamneni A, Camarero S, García-Burgos C, Plou FJ. 2008. Engineering and Applications of fungal laccases for organic synthesis. Microb. Cell Fact. 7, 32. https://doi.org/10.1186/1475-2859-7-32 PMid:19019256 PMCid:PMC2613868

Kurzbaum E, Kirzhner F, Sela S, Zimmels Y. 2010. Efficiency of phenol biodegradation by planktonic Pseudomonas pseudoalcaligenes (a constructed wetland isolate) vs. root and gravel biofilm. Water Res. 44, 5021–5031. https://doi.org/10.1016/j.watres.2010.07.020 PMid:20705318

Lade H, Govindwar S, Paul D. 2015. Low-cost biodegradation and detoxification of textile azo dye C.I. Reactive Blue 172 by Providencia rettgeri Strain HSL1. J. Chem. 2015, 1–10. https://doi.org/10.1155/2015/894109

Liu Z, Xie W, Li D, Peng Y. 2016. Biodegradation of phenol by bacteria strain Acinetobacter Calcoaceticus PA isolated from phenolic wastewater. Int. J. Environ. Res. Public Health 13, 1-8. https://doi.org/10.3390/ijerph13030300

Margot J, Bennati-Granier C, Maillard J, Blanques P. 2013. Bacterial versus fungal laccase: potential for micropollutant degradation. AMB Express 3, 1-14. https://doi.org/10.1186/2191-0855-3-63 PMid:24152339 PMCid:PMC3819643

Marrot B, Barrios-Martinez A, Moulin P, Roche N. 2006. Biodegradation of high phenol concentration by activated sludge in an immersed membrane bioreactor. Biochem. Eng. J. 30, 174–183. https://doi.org/10.1016/j.bej.2006.03.006

Mishra S, Behera N. 2008. Amylase activity of a starch degrading bacteria isolated from soil receiving kitchen wastes. African J. Biotechnol. 7, 3326–3331.

Mongkolthanaruk W, Tongbopit S, Bhoonobtong A. 2012. Independent behavior of bacterial laccases to inducers and metal ions during production and activity. African J. Biotechnol. 11, 9391–9398. https://doi.org/10.5897/AJB11.3042

Mu-oz R, Díaz LF, Bordel S, Villaverde S. 2007. Inhibitory effects of catechol accumulation on benzene biodegradation in Pseudomonas putida F1 cultures. Chemosphere 68, 244–252. https://doi.org/10.1016/j.chemosphere.2007.01.016 PMid:17316748

Muthukumar NP, Murugan S. 2014. Production, purification and application of bacterial laccase: A Review. Biotechnology 13, 196–205. https://doi.org/10.3923/biotech.2014.196.205

Rice EW, Baird RB, Eaton AD, Clesceri LS. 2012. Standard Methods for the Examination of Water and Wastewater. American Public Health Associasion.

Ruiz JC, De la Rubia T, Pérez J, Martinez Lopez, J. 2002. Effect of olive oil mill wastewater on extracellular ligninolytic enzymes produced by Phanerochaete flavido-alba. FEMS Microbiol. Lett. 212, 41–45. https://doi.org/10.1111/j.1574-6968.2002.tb11242.x PMid:12076785

Sondhi S, Sharma P, George N, Chauhan PS. 2015. An extracellular thermo-alkali-stable laccase from Bacillus tequilensis SN4, with a potential to biobleach softwood pulp. 3 Biotech. 5, 175–185.

Sulji? S, Mortzfeld FB, Gunne M, Urlacher VB. 2015. Enhanced biocatalytic performance of bacterial laccase from Streptomyces sviceus: Application in the Michael addition sequence towards 3-Arylated 4-Oxochromanes Chem. Cat. Chem. 7, 1380–1385.

Tafesh A, Najami N, Jadoun J, Halahlih F. 2011. Synergistic antibacterial effects of polyphenolic compounds from olive mill wastewater. Evid. Based. Complement. Alternat. Med. 2011, 1-9. https://doi.org/10.1155/2011/431021 PMid:21647315 PMCid:PMC3106970

Tahmasbi H, Khoshayand MR, Bozorgi-Koushalshahi M, Heidary M. 2016. Biocatalytic conversion and detoxification of imipramine by the laccase-mediated system. Int. Biodeterior. Biodegradation 108, 1–8. https://doi.org/10.1016/j.ibiod.2015.11.029

Verma A, Dhiman K, Shirkot. 2016. Hyper-production of laccase by Pseudomonas putida LUA15.1 through mutagenesis. J. Microbiol. Exp. 3, 1-8. https://doi.org/10.15406/jmen.2016.03.00080

Yuan H, Yao J, Masakorala K, Wang F. 2014. Isolation and characterization of a newly isolated pyrene-degrading Acinetobacter strain USTB-X. Environ. Sci. Pollut. Res. 21, 2724–2732. https://doi.org/10.1007/s11356-013-2221-9 PMid:24122268




Copyright (c) 2018 Consejo Superior de Investigaciones Científicas (CSIC)

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.


Contact us grasasyaceites@ig.csic.es

Technical support soporte.tecnico.revistas@csic.es