Grasas y Aceites, Vol 70, No 1 (2019)

Cucumis melo L. seeds as a promising source of oil naturally rich in biologically active substances: compositional characteristics, phenolic compounds and thermal properties

S. Mallek-Ayadi
Research Group of Agri-Food Processing Engineering, Laboratory of Applied Fluids Mechanics, Process Engineering and Environment, National School of Engineers of Sfax, University of Sfax, Tunisia

N. Bahloul
Research Group of Agri-Food Processing Engineering, Laboratory of Applied Fluids Mechanics, Process Engineering and Environment, National School of Engineers of Sfax, University of Sfax, Tunisia

N. Kechaou
Research Group of Agri-Food Processing Engineering, Laboratory of Applied Fluids Mechanics, Process Engineering and Environment, National School of Engineers of Sfax, University of Sfax, Tunisia


This study aimed to evaluate the characteristic features, phenolic compounds and thermal analysis of melon seed oil (Maazoun variety), in order to determine its potential applications in food or pharmaceutical industries. The physicochemical properties of the seed oil revealed a high degree of unsaturation. The average contents of carotenoid and chlorophyll were 2.43 mg/kg and 5.70 mg/kg, respectively. The main fatty acids of melon seed oil were linoleic acid (68.98%) and oleic acid (15.84%), which makes this oil nutritionally valuable. Furthermore, trilinolein (LLL), accounted for 28.99% and constitutes the most abundant triacylglycerol. A chromatographic analysis showed that amentoflavone and luteolin-7-glycoside were the major phenolic compounds. A thermal analysis of melon seed oil was performed by differential scanning calorimetery (DSC). The results of sensorial properties indicated that melon seed oil is appreciated by tasters. The findings suggested that because of its composition, melon seed oil could be used successfully as an alternative source in the food and nutraceutical industries as a functional ingredient.


Fatty acid composition; Melon (Cucumis melo L.); Phenolic compounds; Phytosterols; Seed oil; Sensory analysis; Thermal profile

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A.O.A.C. 2000. Association of Official Analytical Chemists: Official Methods of Analysis. 17th Edition. Gaithersburg, MD, USA.

A.O.C.S. 2012. American Oil Chemists´ Society. Official Methods and Recommended Practices of the American Oil Chemists' Society. 6th Edition. Champaign, IL, USA.

Aparicio R, Roda L, Albi MA, Gutierrez F. 1999. Effect of various compounds on virgin olive oil stability measured by Rancimat. J. Agric. Food Chem. 47, 4150–4155.

Azhari S, Xu YS, Jiang QX, Xia WS. 2014. Physicochemical properties and chemical composition of Seinat (Cucumis melo var. tibish) seed oil and its antioxidant activity. Grasas Aceites 65, 1–8.

Bahloul N, Kechaou N, Mihoubi NB. 2014. Comparative investigation of minerals, chlorophylls contents, fatty acid composition and thermal profiles of olive leaves (Olea europeae L.) as by-product. Grasas Aceites 65, 3–35.

Carvalho IS, Teixeira MC, Brodelius M. 2011. Fatty acids profile of selected Artemisia spp. plants: Health promotion. LWT -Food Sci. Technol. 44, 293–298.

Cheikh-Rouhou S, Besbes S, Hentati B, Blecker C, Deroanne C, Attia H. 2007. Nigella sativa L.: Chemical composition and physicochemical characteristics of lipid fraction. Food Chem. 101, 673–681.

Chen L, Kang YH. 2013. In vitro inhibitory effect of oriental melon (Cucumis melo L. var. makuwa Makino) seed on key enzyme linked to type 2 diabetes. J. Func. Foods 5, 981–986.

Chouaibi M, Mahfoudhi N, Rezig L, Donsì F, Ferrari G, Hamdi S. 2012. Nutritional composition of Zizyphus lotus L. seeds. J. Sci. Food Agric. 92, 1171–1177.

Chtourou M, Gargouri B, Jaber H, Abdelhedi R, Bouaziz M. 2013. Comparative study of olive oil quality from Chemlali Sfax versus Arbequina cultivated in Tunisia. Eur. J. Lipid Sci. Technol. 115, 631–640.

Conto LC, Gragnani MAL, Maus D, Ambiel HCI, Chiu MC, Grimaldi R. 2011. Characterization of crude watermelon seed oil by two different extractions methods. J. Am. Oil Chem. Soc. 88, 1709–1714.

Decker EA. 1998. Strategies for manipulating the prooxidative/antioxidative balance of foods to maximize oxidative stability. Trends Food Sc. Technol. 9, 241–248.

EEC. 1991. Characteristics of olive and olive pomace oils and their analytical methods. Regulation EEC/2568/1991. Off. J. Eur. Com. L248, 1–82.

FAOSTAT. 2015. FAO Statistical Database.

Gohari Ardabili A, Farhoosh R, Haddad Khodaparast MH. 2011. Chemical Composition and Physicochemical Properties of Pumpkin Seeds (Cucurbita pepo Subsp. pepo Var. Styriaka) Grown in Iran. J. Agric. Sci. Technol. 13, 1053–1063.

Górna? P, Rudzi?ska M. 2016. Seeds recovered from industry by-products of nine fruit species with a high potential utility as a source of unconventional oil for biodiesel and cosmetic and pharmaceutical sectors. Ind. Crops Prod. 83, 329–338.

Górna? P, Siger A, Juhnevi?a K, L?cis G, ?n? E, Segli?a D. 2014. Cold pressed Japanese quince (Chaenomeles japonica (Thunb) Lindl. ex Spach) seed oil as a rich source of ?-tocopherol, carotenoids and phenolics: A comparison of the composition and antioxidant activity with nine other plant oils. Eur. J. Lipid Sci. Technol. 116, 563–570.

Hellier P, Ladommatos N, Yusaf T. 2015. The influence of straight vegetable oil fatty acid composition on compression ignition combustion and emissions. Fuel 143, 131–14.

Hsu SY, Yu SH. 2002. Comparisons on 11 plant oil fat substitutes for low-fat kung-wans. J. Food Eng. 51, 215–220.

Issaoui M, Flamini G, Brahmi F, Dabbou S, Ben Hassine K, Taamali A, Chehab H, Ellouz M, Zarrouk M, Hammami M. 2010. Effect of the growing area conditions on differentiation between Chemlali and Chetoui olive oils. Food Chem. 119, 220–225.

Mallek-Ayadi S, Bahloul N, Kechaou N. 2018. Chemical composition and bioactive compounds of Cucumis melo L. seeds: Potential source for newtrends of plant oils. Process Saf. Environ. Prot. 113, 68–77.

Maran JP, Priya B. 2015. Supercritical fluid extraction of oil from muskmelon (Cucumis melo) seeds. J. Taiwan Inst. Chem. Eng. 47, 71–78.

Mariod AA, Ahmed YM, Matthaus B, Khaleel G, Siddig A, Gabra AM, Abdelwahab SI. 2009. A Comparative Study of the Properties of Six Sudanese Cucurbit Seed and Seed Oils. J. Am. Oil Chem. Soc. 86, 1181–1188.

Nyam KL, Tan CP, Lai OM, Long K, Mana CYB. 2009. Physicochemical properties and bioactive compounds of selected seed oils. Food Sci. Biotechnol. 42, 1396–1403.

O'Brien RD. 2004. Fats and oils: Formulating and processing for applications. Bocca Raton, USA: CRC Press.

Oomah DB, Ladet S, Godfrey DV, Liang J, Girard B. 2000. Characteristics of rasberry (Rubus idaeus L.) seed oil. Food Chem. 69, 187–193.

Ouni Y, Flamini G, Issaoui M, Ben Youssef N, Cioni PL, Hammami M, Daoud D, Zarrouk M. 2011. Volatile compounds and compositional quality of virgin olive oil from Oueslati variety: Influence of geographical origin. Food Chem. 124, 1770–1776.

Rezig L, Chouaibi M, Msaada K, Hamdi S. 2012. Chemical composition and profile characterization of pumpkin Cucurbita maxima seed oil. Ind. Crops Prod. 37, 82–87.

Rocha LD, Monteiro MC, Anderson JT. 2012. Anticancer properties of hydroxycinnamic acids-A Review. Cancer Clin. Oncol. 1, 1927–4866.

Shi C, Sun Y, Zheng Z, Zhang X, Song K, Jia Z, Chen Y, Yang M, Liu X, Dong R, Xia X. 2016. Antimicrobial activity of syringic acid against Cronobacter sakazakii and its effect on cell membrane. Food Chem. 197, 100–106. PMid:26616929

Silva AC da, Jorge N. 2014. Bioactive compounds of the lipid fractions of agro-industrial waste. Food Res. Int. 63, 493– 500.

Singleton VL, Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16, 144–158.

Soltana H, Tekaya M, Amri Z, El-Gharbi S, Nakbi A, Harzallah A, Mechri B, Hammami M. 2016. Characterization of fig achenes' oil of Ficus carica grown in Tunisia. Food Chem. 196, 1125–1130. PMid:26593597

Soong YY, Barlow PJ. 2006. Quantification of gallic acid and ellagic acid from longan (Dimocarpus longan Lour.) seed and mango (Mangifera indica L.) kernel and their effects on antioxidant activity. Food Chem. 97, 524–530.

Tan CP, Che Man YB. 2000. Differential scanning calorimetric analysis of edible oils: Comparison of thermal properties and chemical composition. J. Am. Oil Chem. Soc. 77, 143–155.

Yanty NAM, Lai OM, Osman A, Long K, Ghazali HM. 2008. Physicochemical properties of Cucumis melo var. inodorus (honeydew melon) seed and seed oil. J. Food Lipids 15, 42–55.

Zeb A. 2016. Phenolic Profile and Antioxidant Activity of Melon (Cucumis melo L.) Seeds from Pakistan. Foods 5, 67–74. PMid:28231162

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