Grasas y Aceites, Vol 70, No 3 (2019)

Column chromatography as a method for minor components removal from rapeseed oil


https://doi.org/10.3989/gya.0709182

E. Rokosik
Department of Food Biochemistry and Analysis, Poznań University of Life Sciences, Poland
orcid http://orcid.org/0000-0002-3604-4414

K. Dwiecki
Department of Food Biochemistry and Analysis, Poznań University of Life Sciences, Poland
orcid http://orcid.org/0000-0002-1438-1217

M. Rudzińska
Institute of Food Technology of Plant Origin, Poznań University of Life Sciences, Poland
orcid http://orcid.org/0000-0001-6343-332X

A. Siger
Department of Food Biochemistry and Analysis, Poznań University of Life Sciences, Poland
orcid http://orcid.org/0000-0002-3681-153X

K. Polewski
Department of Physics and Biophysics, Poznań University of Life Sciences, Poland
orcid http://orcid.org/0000-0003-0445-7721

Abstract


The purpose of this study was to verify the influence of different chromatographic column beds (silicic acid, activated charcoal, aluminum oxide, silica gel) on the concentration of individual minor components (sterols, tocopherols, carotenoids and chlorophyll) in rapeseed oil. With the use of a combination of these beds, a three-stage optimized method for removing minor components from rapeseed oil was developed. It was demonstrated that the combination of silicic acid and activated charcoal removed about half of the sterols present from the oil. Aluminum oxide turned out to be the most effective bed in removing tocopherols, purifying the oil to their minimum level (2.6 mg/kg). All adsorbents used had similar capacity to purify oil from pigments (carotenoids and chlorophyll). In the three-stage purification process free sterols were almost completely removed (to the level 90.0 mg/kg). Purification of β-carotene and chlorophyll from the oil was also very effective. Tocopherols were completely removed with this method, except for a small amount of α-tocopherol (0.4 mg/kg), which results from its relatively weak interaction with a hydrophilic bed. The developed method may be used in studies on the effect of association colloids on bulk oil autoxidation processes.

Keywords


Association colloids; Column chromatography; Oil autoxidation; Rapeseed oil; Sterols; Tocopherols

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