Optimization of the enzymatic pre-treatment process for mustard oilseeds using response surface methodology

Authors

DOI:

https://doi.org/10.3989/gya.1284182

Keywords:

Enzymatic pre-treatment, Enzymes, Factorial design, Mustard seeds, Viscozyme

Abstract


In order to investigate the effect of an enzymatic pre-treatment process for the extraction of oil from black mustard seeds (Brassica nigra) using viscozyme, the reaction parameters such as temperature, buffer-to-seed ratio and enzyme concentration were considered as determinant factors in the central composite design. Optimization was carried out according to the four-variable five-level central composite design of experiments. The effects of enzyme concentration (5–12%), temperature (40–55 °C), pH (5.0–6.0), and reaction time (1–7 h) on the free oil liberated were studied. Residual oil was collected by subjecting the treated meal to soxhlet extraction for 4 h. An enzyme dose of 7.5% (w/w), pH 5.0, 50 °C, and 5 h with constant shaking at 450 rpm were found to be opti­mal conditions. Centrifuging the mixture at 7000 rpm for 30 min separated the oil with a recovery of 71–73.1%.

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References

Abdulkarim SM, Lai OM, Muhammad SKS, Long K, Ghazali HM. 2006. Use of enzymes to enhance oil recovery dur­ing aqueous extraction of Moringa oleifera seed oil. J. Food Lipids 13, 113–130.

Abul FMM, Badry NE, Ammar MS. 2011. Nutritional and chemical evaluation for two different varieties of mustard seeds. World Appl. Sci. J. 15, 1225–1233.

Dandjouma AKA, Tchiegang C, Kapseu C, Linder M, Parmentier M. 2008. Enzyme-assisted hexane extraction of Ricinodendron heudelotii (Bail.) Pierre ex Pax seeds oil. Int. J. Food Sci. Technol. 43, 1169–1175.

Danso BE. 2011. Effect of enzyme and heat pretreatment on sunflower oil recovery using aqueous and hexane extrac­tions.Int. J. Chem. Biomol. Eng. 4, 28–34.

Dobozi MS, Halfisz A, Kozma K, Szakacs G. 1988. Enhancement of mustard oil yield by cellulolytic pretreatment. App. Microbiol. Biotechnol. 29, 39–43.

Domínguez H, Nunez MJ, Lema JM. 1994. Enzymatic pre­treatment to enhance oil extraction from fruits and oil­seeds: A review. Food Chem. 49, 271–286.

Fahad MAJ, Mohammed SAJ. 2012. Chemical composition and fatty acid content of some spices and herbs under Saudi Arabia conditions. The Sci. World J. (Article ID: 859892).

Harinder SO, Neha B, Sandeep SD, Simranjeet K, Praveen VV, Vinod KB, Ramabhau TP 2012. Enhanced oil recovery by pretreatment of mustard seeds using crude enzyme extract obtained from mixed-culture solid-state fermentation of kinnow (Citrus reticulata) waste and wheat bran. Food Bioprocess Technol. 5, 759–767.

Koehler J, Marschner V, Winter B. 2008. Improved procedure for the extraction of oil from seeds. PCT Int. Appl. WO 2008080495 A1 20080710

Olsen BS. 1986. Enzyme process for extraction of rape seed oil. Process Development Bulletin No.2. Novo F-865268/HSO.

Pawanpreet S, Singh AK. 2013. Optimization of operational parameters for enhancement of oil recovery from mustard seeds using mechanical expression. Int. J. Sci. Eng. Res. 4, 583–589.

Ravindra KA, John DBS. 2014. Optimization of viscozyme-L assisted extraction of coconut milk and virgin coconut oil. Asian J. Dairy Food Res. 33, 276–284.

Sarkar BC, Pandey S, Kumbhar BK, Agrawal YC. 2004. Aqueous oil extraction from enzyme pretreated sesame seed and process parameters optimization. J. Food Sci. Technol. 41, 604–608.

Sengupta R, Bhattacharyya DK. 1996. Enzymatic extraction of mustard seed and rice bran. J. Am. Oil Chem. Soc. 73, 687–692.

Shabtai Y, Gotshal N, Ramot O, Wisniak J. 1998. Enzymatic pretreatment of jojoba seeds to facilitate oil extraction. Ind. J. Chem. Technol. 5, 124–130.

Shah S, Sharma A, Gupta MN. 2004. Extraction of oil from Jatropha curcas L. seed kernels by combination of ultra­sonication and aqueous enzymatic oil extraction. Biores. Technol. 96, 121–123.

Singh P, Singh AK. 2013. Optimization of operational param­eters for enhancement of oil recovery from mustard seeds using mechanical expression. Int. J. Sci. Eng. Res. 4, 583–589.

Solmaz T, Levente LD. 2013. Aqueous and enzymatic extrac­tion processes for the production of food grade proteins and industrial oil from dehulled yellow mustard flour. Food Res. Int. 52, 547–556.

Stamenković OS,Ivica GD, Milan DK, Petar MM, Vlada BV. 2018. Optimization and kinetic modeling of oil extrac­tion from white mustard (Sinapis alba L.) seeds. Ind. Crops and Prod. 121, 132–141.

Swati, Sneha S, Madhusweta D. 2015. A brief overview: Present status on utilization of mustard oil and cake. Ind. J. Trad. Knowl. 14, 244–250.

Wang JG, Zhang H, Liu F, Liu DY, Pan MZ, Yu DY. 2014. Optimization of enzymatic pretreatment process for pressing of evening primrose oil. Shipin Kexue 35, 96–101.

Zu T, He M, Zhang L. 2013. Study on aqueous enzymatic extrac­tion of oil from rubber seeds. Zhongguo Liangyou Xuebao 28, 37–42.

Published

2020-06-30

How to Cite

1.
Neeharika TS, Prasanna Rani KN, Thirupathi A, Anjaneyulu E, Srikanth K, Prabhavathi Devi BL, Prasad RB, Jala RC. Optimization of the enzymatic pre-treatment process for mustard oilseeds using response surface methodology. grasasaceites [Internet]. 2020Jun.30 [cited 2020Nov.25];71(2):e351. Available from: http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1818

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Research

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