Grasas y Aceites, Vol 71, No 2 (2020)

Camellia oil saponins: Solid phase extraction and its effect on mice blood and organs


https://doi.org/10.3989/gya.1171182

H. O.A. Ahmed
Department of Food Science and Engineering, Institute of Food Science and Technology, Huazhong Agricultural University, China
orcid https://orcid.org/0000-0002-9979-0844

C. M. Wang
Department of Food Science and Engineering, Institute of Food Science and Technology, Huazhong Agricultural University, China
orcid https://orcid.org/0000-0002-2450-3388

A. A. Mariod
Department of Biology, College of Science and Arts, University of Jeddah - Indigenous Knowledge and Heritage Center, Ghibaish College of Science and Technology, Saudi Arabia
orcid https://orcid.org/0000-0003-3237-7948

T. A.A. Hammoda
College of Engineering and Technical Studies, Elimam Elmahadi University, Sudan
orcid https://orcid.org/0000-0001-9227-7880

Abstract


In this study, the tea saponin extracted experiment was adopted to optimize extraction conditions. The solid phase extraction technique was used to extract saponin from camellia tea oil. Four macro-porous resins (DM301; NKA-9; HZ-841; S-8), two cation exchange resins (D001; 732), chitosan and diatomite, respectively, were used in a preliminary experiment; the HZ-841 macro-porous resin was determined as the optimal solid adsorbent material. The extraction conditions were; temperature 23.78 ºC; adsorption time 5.20 hour; liquid-to-solid ratio 12.54:1; predicted adsorption rate 20.20%; ethanol concentration 83.27%; eluent flow rate 1.18 mL/min; liquid-to-solid ratio 21.85:1; and the elution rate of tea saponin was calculated as 59.55%. The effect of tea seed oil saponin on mice blood and organs (liver, kidney, spleen and heart) was studied. The mice were randomly divided into six groups and fed for 90-days. Their weights were recorded every day. On the last day of the experiment serum ele­ments: Total cholesterol (TC), triglycerides (TG), low and high-density lipoproteins (LDL, HDL), as well as AST (aspartate aminotransferase), ALT (alanine aminotransferase) and total protein (TP) and organ (liver, kidney, spleen and heart) histopathologies were determined. The study results demonstrated that tea oil saponin expanded TC, TG, LDL and HDL. However, it generally increased ALT, AST activities and TP values compared to control groups. Tea oil saponin had no effect on organ tissue histopathology compared to control groups.

Keywords


90-days feeding; Camellia seed oil; Solid phase extraction; Tea saponin; Toxicity

Full Text:


HTML PDF XML

References


Abliz A, Aji Q, Abdusalam E, Sun X, Abdurahman A, Zhou W, Moore N, Umar A. 2014. Effect of Cydonia oblonga M ill. Leaf extract on serum lipids and liver function in a rat model of hyperlipidaemia. J. Ethnopharm. 151, 970–974.

Chun Y, Jing Z. 2010. Effect of adsorption separation charac­teristics of macroporous resins for total flavonoids of chry­santhemum. J. Food Sci. 12, 127–131.

Du L, Fu QY, Xiang LP, Zheng XQ, Lu JL, Ye JH, Li QS, Polito CA, Liang YR. 2016. Tea polysaccharides and their bio­activities. Molecules 21, 1449;

Ghule BV, Ghante MH, Saoji AN, Yeole PG. 2009. Antihyperlipide mice effect of the methanolic extract from Lagenaria siceraria stand. Fruit in hyper lipidemic rats. J. Ethnopharmacol. 124, 333–337.

Hostettmann K, Marston A. 1995. Saponins. Cambridge: Cambridge University Press. p. 3ff. ISBN 978-0-521- 32970-5. OCLC 29670810.

Hu JB, Yang GL. 2018. Physiochemical characteristics, fatty acid profile and tocopherol composition of the oil from Camellia oleifera Abel cultivated in Henan, China. Grasas Aceites 69 (2), e255.

Leaves X. 2002. Tea saponin and exploitation. J. Chem. Prod. Technol. 9 (2), 6–8.

Li N. 2007. The statue-sque and development of domestic food safety toxicology evaluation. J. Toxicol. 12, 368–370.

Liu K, Wang J, Gao W, Man S, Wang Y, Liu C. 2013. Preparative separation and purification of steroidal saponins in Paris polyphylla var. yunnanensis by macroporous adsorption resins. Pharm. Biol. 51 (7), 899–905.

Liu J, Yang Z, Lu F, Chen S, Li D. 2016a. Selection of Macroporous Resins for the Separation and Detection of Tomato Saponins. Proceedings of the 2016 6th International Conference on Advanced Design and Manufacturing Engineering (ICADME 2017).

Liu Y, Li Z, Xu H, Han Y. 2016b. Extraction of Saponin from Camellia oleifera Abel Cake by a combination Method of Alkali Solution and Acid Isolation. J. Chem. 2016, Article ID 6903524, 8 pages.

Liu H. 2007. Tea saponin extraction and [D]. Analysis method Changsha: Hunan Agricultural University.

Liyana-Pathirana C, Shahidi F. 2005. Optimization of extrac­tion of phenolic compounds from wheat using response surface methodology. Food Chem. 93, 47–56.

Matsu Y, Kobayashi K, Masuda H. 2009. Quantitative Analysis of Saponins in a Tea-Leaf Extract and Their Antihypercholesterolemic Activity. Biosci. Biotechnol. Biochem. 73 (7), 1513–1519.

NIH. 1997. https://grants.nih.gov/grants/olaw/Guide-for-the- Care-and-use-of-laboratory-animals.pdf.

Pu EY. 1995. Studies on stability of liquid/liquid dispersions: Theory and practice [D]. Zrich: Switzerland Union Higher Industry College; Doctor Degree Thesis.

Qiu H, Chen D, Liu Y, Wu X, Ren R, Cheng Q. 2011. Study on anti atheroscloresis effects of daidai flavones dropping pills on hype-lipidemia rats. Chin. J. Mod. Appl. Pharm. 28, 597–601.

Shen JF, Kang R, Chen YQ. 2010. Study on Extraction and anti-oxidation of Camellia oleifera shell polysaccharide. J. Chinese J. Grain and Oil 25 (8), 51–54.

Shen TT, Wu SX. 2017. Effects of Tea Seed Oil on Hyperlipidemic Rats Induced by High-fat Diet. Food Sci. Technol. Res. 23 (1), 101–109.

Shrivenham RH, Xiao CW. 2005. The chemical structure and biological activity of tea saponins research progress (review). J. Anhui Agri. Univ. 01, 369–372.

Tang L, Feng B, Li H, Chen Y, Shi L, Wang Y. 2008. Research progress of Camellia oleifera saponin. J. Central Pharm. 03, 330–333.

Płotka-Wasylka J, Szczepanska N, de la Guardia M, Namiesnik J. 2015. Miniaturized solid-phase extraction techniques. Trends Anal. Chem. 73, 19–38.

Wu X, Huang Y, Xie Z. 2005. Health functions and prospective of Camellia oil. J. Food Sci. Technol. 5, 94–96.

WuH, Li C, LiZ, Liu R, Zhang A, Xiao Z, Ma L, Li J, Deng S. 2018. Simultaneous extraction of oil and tea saponin from Camellia oleifera Abel. seeds under subcritical water condi­tions. Fuel Proc. Tech.174, 88–94.

Xu DJ, Yan S. 2011. Experimental animals and food safety. Chinese J. Comp. Med. Z 1, 27–29.

Yuan XZ, Meng YT, Zeng GM, Fang YY, Shi JG. 2007. Evaluation of tea-derived bio-surfactant on removing heavy metal ions from dilute wastewater by ion flotation. J. Colloids Surf. A: Physicochem. Eng. Asp. 317, 1–3, 256–261.

Zhao LC, He Y, Deng X, Yang GL, Li W, Liang J, Tang QL. 2012. Response Surface Modeling and Optimization of Accelerated Solvent Extraction of Four Lignans from Fructus Schisandrae. Molecules 17, 3618–3629.




Copyright (c) 2020 Consejo Superior de Investigaciones Científicas (CSIC)

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.


Contact us grasasyaceites@ig.csic.es

Technical support soporte.tecnico.revistas@csic.es