Grasas y Aceites, Vol 71, No 3 (2020)

Solid phase extraction of β-sitosterol and α-tocopherol from sunflower oil deodorizer distillate using desilicated zeolite


https://doi.org/10.3989/gya.0570191

O. Karaoglu
The Scientific and Technological Research Council of Turkey - TUBITAK. - Yildiz Technical University, Chemistry Department, Turkey
orcid https://orcid.org/0000-0003-1670-2085

G. Alpdogan
Yildiz Technical University, Chemistry Department, Turkey
orcid https://orcid.org/0000-0002-8433-4352

I. S. Ozdemir
The Scientific and Technological Research Council of Turkey - TUBITAK, Turkey
orcid https://orcid.org/0000-0002-6159-0847

E. Ertas
The Scientific and Technological Research Council of Turkey - TUBITAK, Turkey
orcid https://orcid.org/0000-0002-9736-0824

Abstract


In this study, the efficiency of using zeolite-based adsorbents in a solid phase extraction (SPE) procedure of α-tocopherol and β-sitosterol isolation from Sunflower Oil Deodorizer Distillate (SuDOD) with­out pre-treatment was investigated. The results showed that 99.2% α-tocopherol and 97.3% β-sitosterol were suc­cessfully isolated as pure fractions from SuDOD, when desilicated ZSM-5-type zeolite (DSiZSM-5) was used as adsorbent on a SPE. A simple and rapid HPLC method for simultaneous α-tocopherol and β-sitosterol analysis was developed and validated according to AOAC guidelines. It was found that the inclusion of a DSiZSM-5 SPE step increased the precision of the α-tocopherol and β-sitosterol analysis. In conclusion, DSiZSM-5 zeo­lite was proven to be an efficient adsorbent which can be used not only for the recovery of α-tocopherol and β-sitosterol from SuDOD in industrial scale, but also in a laboratory scale clean-up method prior to the analysis of α-tocopherol and β-sitosterol.

Keywords


SPE; Sterol; Sunflower oil deodorizer distillate; Tocopherol; Zeolite

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