Grasas y Aceites, Vol 54, No 1 (2003)

Enzymatic aqueous technology for simultaneous coconut protein and oil extraction

Beatriz P.M. Sant’Anna

Suely P. Freitas

Maria A. Z. Coelho
Escola de Química, Universidade Federal do Rio de Janeiro, Brazil


In this study, the following commercial enzymes were evaluated in the enzymatic extraction of oil and protein from coconut: Celluclast, Termamyl, Viscozyme, Neutrase y Protease. Preliminary experiments were carried out for selection of enzymes, enzyme concentration and incubation time. In order to verify the effects of pH and water/substrate ratio, a response surface methodology was applied based in a 32 full factorial experimental design. The pH is the most meaningful parameter on oil and protein extraction yields, with a significance level higher than 90%. The maximal extraction yields of oil and protein emulsion (83%) was reached using Viscozyme L and subsequently Neutrase 1.5 MG at concentrations of 0.6% (w/w) and 0.3% (w/w), respectively, total incubation time of 60 minutes, substrate/water ratio 1:6 and pH around 7 (no adjustment).


Aqueous extraction; Coconut oil; Coconut protein; Enzyme

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Copyright (c) 2003 Consejo Superior de Investigaciones Científicas (CSIC)

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