Enzymatic aqueous technology for simultaneous coconut protein and oil extraction

Authors

  • Beatriz P.M. Sant’Anna
  • Suely P. Freitas
  • Maria A. Z. Coelho Escola de Química, Universidade Federal do Rio de Janeiro

DOI:

https://doi.org/10.3989/gya.2003.v54.i1.281

Keywords:

Aqueous extraction, Coconut oil, Coconut protein, Enzyme

Abstract


In this study, the following commercial enzymes were evaluated in the enzymatic extraction of oil and protein from coconut: Celluclast, Termamyl, Viscozyme, Neutrase y Protease. Preliminary experiments were carried out for selection of enzymes, enzyme concentration and incubation time. In order to verify the effects of pH and water/substrate ratio, a response surface methodology was applied based in a 32 full factorial experimental design. The pH is the most meaningful parameter on oil and protein extraction yields, with a significance level higher than 90%. The maximal extraction yields of oil and protein emulsion (83%) was reached using Viscozyme L and subsequently Neutrase 1.5 MG at concentrations of 0.6% (w/w) and 0.3% (w/w), respectively, total incubation time of 60 minutes, substrate/water ratio 1:6 and pH around 7 (no adjustment).

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Published

2003-03-30

How to Cite

1.
Sant’Anna BP, Freitas SP, Coelho MAZ. Enzymatic aqueous technology for simultaneous coconut protein and oil extraction. Grasas aceites [Internet]. 2003Mar.30 [cited 2024Mar.29];54(1):77-80. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/281

Issue

Section

Research