Grasas y Aceites, Vol 59, No 4 (2008)

Production of structured lipids by enzymatic transesterification of soybean oil and palm kernel oil in a packed-bed reactor


https://doi.org/10.3989/gya.2008.v59.i4.527

Nelson Moreno Safra
Centro de Investigación en Ciencia y Tecnología de Alimentos CICTA Escuela de Química, Universidad Industrial de Santander, Bucaramanga, Colombia

Aide Perea Villamil
Centro de Investigación en Ciencia y Tecnología de Alimentos CICTA Escuela de Química, Universidad Industrial de Santander, Bucaramanga, Colombia

Abstract


Enzymatic synthesis of structured lipids by transesterification of soybean oil with palm kernel oil was evaluated in a packed-bed reactor with a capacity for 500g of enzyme loading. Lipozyme RM-IM was used as catalyst. Substrate blends were passed through the enzyme bed at different flow rates. Transesterification reached a level of 19.6 %, with a maximum calculated productivity of 2344 kg of transesterified oil/kg of immobilized enzyme, a flow rate of 9,36 kg oil/kg enzyme/h. The triacylglycerols formed in major proportion were C40:2, C42:2, C42:3, C44:2, C44:3, C50:3 and C50:4. Stereoespecific analysis of the fat before and after transesterification shows a slight migration of acyl groups. The products obtained by this technology can be applied in the formulation of lipid emulsions for enteral and parenteral nutrition and the food industry.

Keywords


Lipase; Lipozyme; Packed-bed reactor; RM-IM; Structured lipids

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