Grasas y Aceites, Vol 60, No 1 (2009)

Evaluation of canola seeds of different cultivars with special emphasis on the quantification of erucic acid and glucosinolates

Sadia Ali
Department of Chemistry and Biochemistry, University of Agriculture, Pakistan

Farooq Anwar
Department of Chemistry and Biochemistry, University of Agriculture, Pakistan

Samia Ashraf
Department of Chemistry and Biochemistry, University of Agriculture, Pakistan

Farah Naz Talpur
National Center of Excellence in Analytical Chemistry, University of Sindh, Pakistan

Muhammad Ashraf
Department of Botany, University of Agriculture, Pakistan


This study reports the characterization of the seeds and seed oils of five locally grown canola cultivars: Zafar-2002, Bulbul (Frontier), Dunkeld, Oscar and Con-11. The oil contents from canola seeds ranged from 34.3 to 39.3%. The levels of protein, fiber, ash and moisture were found to be 22.1-41.0, 12.0-14.0, 3.0-3.5 and 4.0-7.5%, respectively. The glucosinolate (GSL) contents in the canola seeds examined ranged from 49.7 to 78.1 mmol g-1. The extracted canola seed oils revealed an iodine value of 118.2-124.6 g of I/100g of oil; refractive index (40 °C), 1.460-1.464; density (24 °C), 0.914-0.919 mg mL–1; saponification value, 187-195; unsaponifiable matter, 0.51-1.10%; acidity (% as oleic acid), 0.40-1.40, and color (1-in. cell), 1.35-1.73 R + 21.0-38.0 Y. Peroxide value (meq/ kg of oil) and specific extinctions at 232 and 270 nm were determined to be 2.00-7.08, 2.17-3.16 and 0.44-0.91, respectively. The seed oils of the five canola cultivars mainly consisted of oleic (C18:1), linoleic (C18:2) and linolenic (C18:3) acids at levels of 49.16-62.14, 14.61, 23.45 and 6.97-9.10%, respectively. The concentrations of palmitic (C16:0), stearic (C18:0), erucic (C22:1) and gadoleic (C20:1) acids ranged from 3.47 to 6.00, 1.51 to 2.10, traces to 13.03 and 1.30 to 10.63%, respectively. A small amount of arachidic acid (20:0) with a contribution below 1% was also detected. The contents of tocopherols (α, γ, and δ) in the canola oils accounted for 77.1-270.3, 191-500, 3.5-15.6 mg kg-1, respectively.The presence of rather high levels of erucic acid and GSL in the present analysis of canola emphasized the need to further reduce the contents of these two antinutritional constituents in the investigated cultivars.


Canola; Characterization; Erucic acid; Glucosinolates Physicochemical properties; Oxidative stability; Tocopherols

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ISO. 1977. International Organization for Standardization (ISO) Oilseed residues- determination of total ash, International Organization for Standardization, Geneva.

IUPAC. 1987. Standard methods for the analysis of oils, fats and derivatives, International Union of Pure and Applied Chemistry (IUPAC). 7th Rev. Enlarged Ed. edited by C. Paquot and A. Hautfenne, Blackwell Scientific, London.

Kramer JKG, Mahadevan S, Hunt JR, Sauer FD, Corner AH, and Charlton KM.1973. Growth rate, lipid composition, metabolism and myocardial lesions of rats fed rapeseed oils (Brassica campestris var. Arlo, Echo and Span, and B. napus var. Oro). J. Nut. 103, 1696-1708.

Kris-Etherton PM. 1999. Monounsaturated Fatty Acids and Risk of Cardiovascular Disease. Circulation Journal of the American Heart Association 1253-1258.

Larsen LM, Sorensen H. 1985. The value of oilseed rape production in Denmark and the EEC. in Sørensen H, (ed.) Advances in the Production and Utilization of Cruciferous Crops, 1-18. Martinus Nijhoff / Dr W. Junk Publishers, Denmark.

Lee BL, New AL, Ong CN. 2003. Simultaneous determination of tocotrienols, tocopherol, retinol and major carotenoids in human plasma. Clin. Chem. 49, 2056-2066. doi:10.1373/clinchem.2003.022681 PMid:14633878

Lovejoy, JC (2002). “The influence of dietary fat on insulin resistance”. Current Diabetes Reports 2,435-440. doi:10.1007/s11892-002-0098-y PMid:12643169

Manzoor M, Anwar F, Iqbal T, Bhanger MI. 2007. Physicochemical characterization of Moringa concanensis seeds and seed oil. J. Am.Oil Chem. Soc. 84, 413-419. doi:10.1007/s11746-007-1055-3

Markwell PJ, Bauer JER, Senior DF. 1998. The impact of dietary fats and poly unsaturated fatty acids on renal disease. Anim. Physiol. Nutr. 80, 234-238.

McGinely L.1991. Analysis and quality control for processing and processed fats, in Rossell, JB, Pritchard JLR, (Eds.), Analysis of Oilseeds, Fats and Fatty Foods, 460-470, Elsevier Applied Science, New York.

Neff W, Mounts TL, Rinsch WM. 1997. Oxidative stability as affected by triacylglycerol composition and structure of purified canola oil triacylglycerols from genetically modified normal and high stearic and lauric acid canola varieties. Lebensmittel-Wissenschaft und-Technologie 30, 793-799.

PARC. Pakistan Agricultural Research Council (PARC). 2007. Rapeseed-Mustard (Brassicaspecies)

Pritchard JLR. 1991. Analysis and properties of oilseeds. in Rossell, JB, Pritchard JLR, (Eds.), Analysis of Oilseeds, Fats and Fatty Foods, 39-102, Elsevier Applied Science, New York.

Pritchard FM, Eagles A, Norton RM, Salisbury PA, Nicolas M. (2000). Environmental Effects on seed composition of Victorian Canola, Aus. J. Exp. Agric. 40, 679-685. doi:10.1071/EA99146

Rossell, JB. 1991. Vegetable oil and fats. in Rossell, JB, Pritchard JLR, (Eds.), Analysis of Oilseeds, Fats and Fatty Foods, 261-319, Elsevier Applied Science, New York.

Sarwar G, Blair R, Friedman M, Gumbmann MR, Hackler LR, Pellett PL, Smith TK. 1984. Inter and intralaboratory variability in rat growth assays for estimating protein quality of foods. J. Assoc. Off. Anal. Chem. 67, 976-981.

Schaefer EJ. 2002. Lipoproteins, nutrition, and heart disease. Am. J. Clin. Nutr. 75, 191-212.

Shahidi F, Shukla VKS.1996. Non-triacylglycerol constituents of fats and oils. International News on Fats, Oils and Related Materials, 7, 1227-1231.

Smith CA, Dacembe C. 1987. Rapid method for determination of total glucosinolates in rapeseed by measuring of enzymatically released glucose. J. Sci. Food Agric. 38,141-150. doi:10.1002/jsfa.2740380206

Zambiazi RC. 1997. The role of endogenous lipid components on vegetable oil stability. PhD. Thesis, University of Manitoba, Winnipeg, Manitoba, Canada.

Zukalova H, Vasak J. 2002. The role and effects of glucosinolates of Brassica species-a review. Rostlinna Vyroba 48, 175-180

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