Grasas y Aceites, Vol 48, No 4 (1997)

Determination of sterols and triterpenic dialcohols in olive oils using HPLC separation and GC analysis. Standardization of the analytical method.


https://doi.org/10.3989/gya.1997.v48.i4.791

Arturo Cert
Instituto de la Grasa (C.S.I.C)., Spain

Wenceslao Moreda
Instituto de la Grasa (C.S.I.C)., Spain

Josep García Moreno
Agra S.A., Spain

Abstract


A method is developed for the analysis of sterols together with triterpenic alcohols (erythrodiol and uvaol) in vegetable oils. The method involves obtention of the unsaponifiable matter, isocratic HPLC separation with refractive index detector, preparation of silylderivatives, and analysis by capillary GC. Four procedures involving GC analysis of sterol and sterol+dialcohol fractions obtained by HPLC and CCF separations are compared. A collaborative trial comparing HPLC-sterols+dialcohols and CCF-sterols (official) methods is carried out. For Δ7-sterols, higher values and better repeatability and reproducibility were observed in the first method, but no differences for the remainder sterols were found. The erythrodiol+uvaol determinations by HPLC-sterols+dialcohols and CCF-sterols+dialcohols (official) methods resulted in similar precision.

Keywords


Collaborative trial Sterols; Uvaol.

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