Grasas y Aceites, Vol 61, No 2 (2010)

Secoiridoids in olive seed: characterization of nüzhenide and 11-methyl oleosides by liquid chromatography with diode array and mass spectrometry

Sandra Silva
Instituto de Biologia Experimental e Tecnológica - Instituto de Tecnologia Química e Biológica - Faculdade de Farmácia de Lisboa, Portugal

Lucília Gomes
Instituto de Biologia Experimental e Tecnológica - Faculdade de Farmácia de Lisboa, Portugal

Fausto Leitão
Instituto Nacional de Recursos Biológicos I.P., Unidade de Recursos Genéticos, Ecofisiologia e Melhoramento de Plantas, Portugal

Maria Bronze
Instituto de Biologia Experimental e Tecnológica - Instituto de Tecnologia Química e Biológica - Faculdade de Farmácia de Lisboa, Portugal

Ana V. Coelho
Instituto de Tecnologia Química e Biológica - Universidade de Évora, Portugal

Luís Vilas Boas
Instituto de Biologia Experimental e Tecnológica - Instituto de Tecnologia Química e Biológica, Portugal


The seed extracts of olive tree cultivars were analyzed by reverse phase HPLC with diode array detection and mass spectrometry. HPLC hyphenation with mass spectrometry (ESI source) in both polarity modes enabled the identification in olive seeds of nüzhenide and nüzhenide 11-methyl oleoside, among other 11-methyl oleosides, by means of MSn. The methods used allowed us to obtain olive seed profiles of phenolic components and to conclude that they are mainly secoiridoids. The quantification of detected phenolic secoiridoids was also achieved using ultraviolet detection (λ = 240 nm) which enabled comparison of the samples. Nüzhenide and nüzhenide 11-methyl oleoside were the major components detected in olive seeds of all the cultivars studied, but variations in individual components of olive seeds were verified among the cultivars. The results also support the existence of di and tri(11-methyl oleosides) of nüzhenide.


Mass spectrometry; 11-Methyl oleosides; Nüzhenide; Olive seed; Phenolic compounds; Secoiridoids

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