Grasas y Aceites, Vol 46, No 4-5 (1995)

Fractionation of phenolase from green table olives (Ascolana tenera var.) by immobilized copper affinity chromatography


https://doi.org/10.3989/gya.1995.v46.i4-5.933

V. Sciancalepore
Dipartimento di Scienze e Tecnologie Agro-Alimentari, Ambientan e Microbiologiche - Universitá del Molise, Italy

G. De Stefano
Dipartimento di Scienze e Tecnologie Agro-Alimentari, Ambientan e Microbiologiche - Universitá del Molise, Italy

Abstract


Phenolase from green table olives (Ascolana tenera var.) was fractionated by immobilized copper affinity chromatography. Four chromatographic fractions with high specific activities were obtained. The highest activity was found for the fraction eluted with L-histidine buffer. The four active fractions were characterized by. their response to both specificity and concentration of the substrate.

Keywords


Ascolana tenera var; Green table olive; Immobilized copper affinity chromatography; Phenolase (fractionation)

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