Grasas y Aceites, Vol 46, No 4-5 (1995)

A rapid method for squalene determination in vegetable oils


https://doi.org/10.3989/gya.1995.v46.i4-5.937

A. Lanzón
Instituto de la Grasa y sus Derivados. C.S.I.C., Spain

A. Guinda
Instituto de la Grasa y sus Derivados. C.S.I.C., Spain

T. Albi
Instituto de la Grasa y sus Derivados. C.S.I.C., Spain

C. de la Osa
Instituto de la Grasa y sus Derivados. C.S.I.C., Spain

Abstract


A simple and rapid analytical method for the quantification of squalene in vegetable oils is described. The method consists of a cold alkaline methylation of the oil sample diluted with hexane followed by quantitation by gas chromatography with internal standard. A calibration curve obtained using different standard solutions, and coefficients of variation for three different samples with high, medium and low content of squalene, are reported.

Keywords


Gas chromatography; Squalene (determination); Vegetable oil

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