Grasas y Aceites, Vol 61, No 4 (2010)

Reduction in hypercholesterolemia and risk of cardiovascular diseases by mixtures of plant food extract: a study on plasma lipid profile, oxidative stress and testosterone in rats


https://doi.org/10.3989/gya.021210

Doha A. Mohamed
Food Sciences and Nutrition Department, National Research Centre, Egypt

Thanaa E. Hamed
Food Sciences and Nutrition Department, National Research Centre, Egypt

Sahar Y. Al-Okbi
Food Sciences and Nutrition Department, National Research Centre, Egypt

Abstract


The present study was postulated to prepare and evaluate the influence of two plant food extract mixtures on plasma lipid profile, oxidative stress and testosterone levels in rats fed a hypercholesterolemic diet. The safety of the studied extract mixtures was evaluated through the determination of liver and kidney functions. The total phenolic contents, tocopherols, fatty acids and unsaponifiable matter (UNSAP) in the extract mixtures were determined. Rats fed a hypercholesterolemic diet were given a daily oral dose (300 mg/kg rat body weight) of either mixture I or II for a month and compared with a control hypercholesterolemic group and a normal control group. Results showed that α-tocopherol was 0.750 and 4.017 mg, γ-tocopherol was 0.564 mg and 0 and δ-tocopherol was 15.23mg and 0.634mg/100g for mixtures I and II, respectively. The phenolic contents in mixtures I and II were 36.74 and 23.72 g gallic acid equivalent/100g mixture, respectively. The GLC investigation of UNSAP revealed that stigmasterol and b-sitosterol were the major phytosterols in mixtures I and II, respectively followed by campesterol in both. The GLC analysis of the fatty acids showed that oleic acid was the major fatty acid in both extract mixtures. Results from the animal experiment showed that feeding a hypercholesterolemic diet produced a significant increase in total lipids, total cholesterol (T-Ch), triglycerides (TGs), low density lipoprotein cholesterol (LDLCh), T-Ch/HDL-Ch, TGs/HDL-Ch and malondialdehyde (MDA) and a significant reduction in high density lipoprotein cholesterol (HDL-Ch), vitamin E, b-carotene and testosterone. Rats fed a hypercholesterolemic diet and given mixture I or II showed significant improvements in plasma lipid profile compared to the hypercholesterolemic control group. This improvement was associated with a significant reduction in oxidative stress reflected by an elevation in plasma levels of antioxidants (vitamin E and b-carotene) and a reduction in plasma MDA levels. The plasma level of testosterone increased significantly in the rats fed the hypercholesterolemic diet and given mixture I or II compared to the hypercholesterolemic control. Plasma testosterone showed a significant negative correlation with plasma TGs and TGs/HDL-Ch in the hypercholesterolemic control rats. The studied extract mixtures showed complete safety towards liver and kidney functions. In conclusion the tested extract mixtures showed an improvement in the plasma lipid profile, a significant increase in testosterone and a decrease in oxidative stress with promising prevention of atherosclerosis and cardiovascular diseases. The antiatherogenic effect of the extract mixtures may be due to the presence of phenolic compounds, phytosterols, tocopherols and unsaturated fatty acids.

Keywords


Extract mixtures; Hypercholesterolemia; Lipid profile; Oxidative stress; Testosterone

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References


AbuMweis SS, Vanstone CA, Ebine N, Kassis A, Ausman LM, Jones PJ, Lichtenstein AH. 2006. Intake of a single morning dose of standard and novel plant sterol preparations for 4 weeks does not dramatically affect plasma lipid concentrations in humans. J. Nutr. 136, 1012-6. PMid:16549466

Alhassan S, Reese KA, Mahurin J, Plaisance EP, Hilson BD, Garner JC, Wee SO, Grandjean PW. 2006. Blood lipid responses to plant stanol ester supplementation and aerobic exercise training. Metabolism 55, 541-9. doi:10.1016/j.metabol.2005.11.006 PMid:16546486

Amaral JS, Casal S, Torres D, Seabra RM, Oliveira BP. 2005. Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method. Analytical Sciences 21, 1545-1548. doi:10.2116/analsci.21.1545 PMid:16379404

AOAC. 1995. Official Methods of Analysis (15th ed.). (Association of Official Agricultural Chemists, Gaithersburg, MD).

A.O.A.C. 2000. Official Methods of Analysis (17th ed.). (Association of Official Agricultural Chemists, Gaithersburg, MD).

Ardiansyah SH, Koseki T, Ohinata K, Hazhizume K, Komai M. 2006. Rice bran fractions improve blood pressure, lipid profile, and glucose metabolism in stroke-prone spontaneously hypertensive rats. Journal of Agricultural and Food Chemistry 54, 1914–1920. doi:10.1021/jf052561l PMid:16506853

Augusti KT, Narayanan A, Pillai LS, Ebrahim RS, Sivadasan R, Sindhu KR. 2001. Beneficial effects of garlic (Allium sativum Linn) on rats feed with diets containing cholesterol and either of the oil seeds, coconuts or groundnuts. Indian J. Exp. Biol. 39, 660-7. PMid:12019759

Briggs GM, Williams MA. 1963. A new mineral mixture for experimental rat diets and evaluation of other mineral mixtures. Fed. Proc. 22, 261-266.

Burstein M, Scholnick HR, Morfin R. 1980. Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions. Scand. J. Clin. Lab. Invest. 40, 583-595.

Carluccio MA, Massaro M, Bonfrate C, Siculella L, Maffia M, Nicolardi G, Distante A, Storelli C, De Caterina R. 1999. Oleic acid inhibits endothelial activation: direct vascular antiatherogenic mechanism of a nutritional component in the Mediterranean diet. Arterioscler Thromb. Vasc. Biol. 19, 220– 8. PMid:9974401

Chinetti G, Lestavel S, Bocher V, Remaley AT, Neve B, Torra IP, Teissier E, Minnich A, Jaye M, Duverger N, Brewer HB, Fruchart JC, Clavey V, Staels B. 2001. PPAR-alpha and PPAR-gamma activators induce cholesterol removal from human macrophage foam cells through stimulation of the ABCA1 pathway. Nat. Med. 7, 53–8. doi:10.1038/83348 PMid:11135616

Christon RA. 2003. Mechanisms of action of dietary fatty acids in regulating the activation of vascular endothelial cells during atherogenesis. Nutr. Rev. 61, 272–9. doi:10.1301/nr.2003.aug.272-279 PMid:13677589

da Luz PL, Favarato D, Faria-Neto JR Jr, Lemos P, Chagas AC. 2008. High ratio of triglycerides to HDLcholesterol predicts extensive coronary disease. Clinics (Sao Paulo). 63, 427-32.

Dauchet L, Amouyel P, Dallongeville J. 2009. Fruits, vegetables and coronary heart disease. Nat. Rev. Cardiol. 6, 599-608. doi:10.1038/nrcardio.2009.131 PMid:19652655

Day RM, Lee YH, Park AM, Suzuki YJ. 2006. Retinoic acid inhibits airway smooth muscle cell migration. Am. J. Respir. Cell Mol Biol. 34, 695–703. doi:10.1165/rcmb.2005-0306OC PMid:16456186    PMCid:1831536

de Jong A, Plat J, Ltjohann D, Mensink RP. 2008. Effects of long-term plant sterol or stanol ester consumption on lipid and lipoprotein metabolism in subjects on statin treatment. Br. J. Nutr. 100, 937-41. doi:10.1017/S0007114508966113 PMid:18846701

Desia I.D, Machlin LJ. 1985. Vitamin E. In: Methods of Vitamin Assay. (eds. J. Augustin, B.P. Klein, D. Becker, P.B. Venugopal). 4th edition. A widely Interscience Publication John Wiley and Sons, New York, p. 255-275.

Devi R, Jayalekshmy A, Arumughan C. 2007. Antioxidant efficacy of phytochemical extracts from defatted rice bran in the bulk oil system. Food Chemistry 104, 658–664. doi:10.1016/j.foodchem.2006.12.014

Duthie GG, Duthie SJ, Kyle JAM. 2000. Plant polyphenols in cancer and heart disease: Implications as nutritional antioxidants. Nutr. Res. Rev. 13, 79-106. doi:10.1079/095442200108729016 PMid:19087434

Elson CE, Peffley DM, Hentosh P, Mo H. 1999. Isoprenoidmediated inhibition of mevalonate synthesis: potential application to cancer. Proc. Soc. Exp. Biol. Med. 22, 294–310. doi:10.1046/j.1525-1373.1999.d01-87.x PMid:10460692

Fawcett JK, Scott JE. 1960. A rapid and precise method for the determination of urea. J. Clin. Pathol. 13, 156-159. doi:10.1136/jcp.13.2.156 PMid:13821779    PMCid:480024

Fernández-Robredo P, Rodriguez JA, Sádaba LM, Recalde S, Garcìa-Layana A. 2008. Egg Yolk improves lipid profile, lipid peroxidation and retinal abnormalities in a murine model of genetic hypercholesterolemia. J. Nutr. Biochem. 19, 40-48. doi:10.1016/j.jnutbio.2006.12.020 PMid:17531457

Fuhrman B, Elis A, Aviram M. 1997. Hypocholesterolemic effect of lycopene and b-carotene is related to suppression of cholesterol synthesis and augmentation of LDL receptor activity in macrophages. Biochem Biophys Res. Commun. 233, 658–662. doi:10.1006/bbrc.1997.6520 PMid:9168909

Fujiwara Y, Takaki A, Uehara Y, Ikeda T, Okawa M, Yamauch K, Ono M, Yoshimitsu H, Nohara T. 2004. Tomato steroidal alkaloid glycosides, esculeoside A and B, from ripe fruits. Tetrahedron 60, 4915– 4920. doi:10.1016/j.tet.2004.03.088

Fujiwara Y, Kiyota N, Hori M, Matsushita S, Iijima Y, Aoki K, Shibata D, Takeya M, Ikeda T, Nohara T, Nagai R. 2007. Esculeogenin A, a New Tomato Sapogenol, Ameliorates Hyperlipidemia and Atherosclerosis in ApoE-Deficient Mice by Inhibiting ACAT.Arterioscler Thromb. Vasc. Biol. 27, 2400-2406. doi:10.1161/ATVBAHA.107.147405 PMid:17872457

Gerard T, Gerald AL. 1981. Process and reagents for the selective separation of low density lipoprotein (LDL) and for the quantification of their components. Eur. Path. (Appl.) E.P. 76, 211-223.

Gokkusu C, Mostafazadeh T. 2003. Changes of oxidative stress in various tissues by long-term administration of vitamin E in hypercholesterolemic rats. Clin. Chim. Acta 328, 155-161. doi:10.1016/S0009-8981(02)00388-1

Goldberg DM, Hahn SE, Parkes J.G. 1995. Beyond alcohol: Beverage consumption and cardiovascular mortality. Clin. Chim. Acta 237, 155-87. doi:10.1016/0009-8981(95)06069-P

Granner DK. 1996. Hormones of the adrenal cortex. In: “Harper’s biochemistry”. Ed. Murray RK, Granner Dk, Rodwell VW, 24th edition, Appleton and Lange, Los Altos, California, p. 547-559.

Guo F, Xu CJ. 2005. Progress on the study of mechanism of the direct action of TCM bioactive components on ovarian cancer. Zhongguo Zhong Xi Yi Jie He Za Zhi 25, 1140-4. PMid:16398441

Gutteridge JMC, Halliwell B. 2000. Free radicals and antioxidants in the year 2000: a historical look to the future. Annals of the New York Academy of Sciences. 899, 136-147. doi:10.1111/j.1749-6632.2000.tb06182.x

Harari A, Harats D, Marko D, Cohen H, Barshack I, Kamari Y, Gonen A, Gerber Y, Ben-Amotz A, Shaish A. 2008. A 9-cis b-Carotene–Enriched Diet Inhibits Atherogenesis and Fatty Liver Formation in LDL Receptor Knockout Mice. J. Nutr. 138, 1923–1930. PMid:18806102

Houot O. 1985. Interpretation of clinical laboratory tests. Edit. Siest G, Henny J, Schiele F, Young D S. Biomedical publications.

Ji JD, Cheon H, Jun JB, Choi SJ, Kim YR, Lee YH, Kim TH, Chae IJ, Song GG, Yoo DH. 2001. Effects of peroxisome proliferator-activated receptorgamma (PPAR-gamma) on the expression of inflammatory cytokines and apoptosis induction in rheumatoid synovial fibroblasts and monocytes. J. Autoimmun 17, 215–21. doi:10.1006/jaut.2001.0542 PMid:11712859

Kaliora AC, Dedoussis GV. 2007. Natural antioxidant compounds in risk factors for CVD. Pharmacological Research 56, 99-109. doi:10.1016/j.phrs.2007.04.018 PMid:17572098

Khandelwal S, Demonty I, Jeemon P, Lakshmy R, Mukherjee R, Gupta R, Snehi U, Niveditha D, Singh Y, van der Knaap HC, Passi SJ, Prabhakaran D, Reddy KS. 2009. Independent and interactive effects of plant sterols and fish oil n-3 long-chain polyunsaturated fatty acids on the plasma lipid profile of mildly hyperlipidaemic Indian adults. Br. J. Nutr. 102, 722-32. doi:10.1017/S0007114509297170 PMid:19296875

Khatoon S, Gopalakrishna AG. 2004. Fat-soluble nutraceuticals and fatty acid composition of selected Indian rice varieties. Journal of the American Oil Chemists’ Society 81, 939–943.

Koch TC, Goldman IL. 2005. Relationship of carotenoids and tocopherols in a sample of carrot root-color accessions and carrot germplasm carrying Rp and rp alleles. J. Agric. Food Chem. 53, 325-31. doi:10.1021/jf048272z PMid:15656668

Kris-Etherton PM, Hecker KD, Bonamone A, Coval SM, Binkoski AE, Hilpert KF. 2002. Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. Am. J. of Med. 113, 71-88. doi:10.1016/S0002-9343(01)00995-0

Löliger J. 1991. The use of antioxidants in food. In: Free radicals and food additives. Aruoma OI., Halliwell B. Eds. Taylor and Francis, London, pp. 129-138.

Marx N, Sukhova GK, Collins T, Libby P, Plutzky J. 1999. PPARalpha activators inhibit cytokine-induced vascular cell adhesion molecule-1 expression in human endothelial cells. Circulation 99, 3125–31. PMid:10377075

Mayes PA. 1996. Structure and function of the lipidsoluble vitamins. In “Harper’s Biochemistry” Edit. Murray RK, Granner DK, Mayes PA, Rodwell VW. 23rd edition, Appleton and Lange, California. p. 614.

Megraw R, Dunn D, Biggs H. 1979 Manual and continuous flow colorimetry of triglycerols by a fully enzymatic method. Clin. Chem. 25, 273-284. PMid:759021

Melendez-Martinez AJ, Vicario IM, Heredia FJ. 2004. Nutritional importance of carotenoid pigments. Arch. Latinoam. Nutr. 54,149–54. PMid:15586682

Mirmiran P, Noori N, Zavareh MB, Azizi F. 2009. Fruit and vegetable consumption and risk factors for cardiovascular disease. Metabolism 58, 460-8. doi:10.1016/j.metabol.2008.11.002 PMid:19303965

Mohamed DA, Al-Okbi SY. 2008 Evaluation of anti-gout activity of some plant foods. Pol. J. Food Nutr. Sci. 58, 389-395.

Momin RA, Nair MG. 2002. Antioxidant, cyclooxygenase and topoisomerase inhibitory compounds from Apium graveolens Linn. seeds. Phytomedicine 9, 312-8. doi:10.1078/0944-7113-00131 PMid:12120812

Morcos SR. 1967 The effect of protein value of the diet on the neurological manifestations produced in rats by b–immodipropionitrile. Br. J. Nutr. 21, 269-274. doi:10.1079/BJN19670029 PMid:4952258

Mruk JS, Webster MW, Heras M, Reid JM, Grill DE, Chesebro JH. 2000. Flavone-8-acetic acid (Flavonoid) profoundly reduces platelet-dependent thrombosis and vasoconstriction after deep arterial injury In vivo. Circulation 101, 324-8. PMid:10645930

Naemura A, Ura M, Yamashita T, Arai R, Yamamoto J. 2008. Long-term intake of rosemary and common thyme herbs inhibits experimental thrombosis without prolongation of bleeding time. Thrombosis Research 122, 517-522. doi:10.1016/j.thromres.2008.01.014 PMid:18378282

Neeld JB, Pearson WN. 1963. Macro and micro methods for the determination of serum vitamin A using trifluroacetic acid. J. Nutr. 79, 454-462. PMid:13937886

Nicolle C, Cardinault N, Gueux E, Jaffrelo L, Rock E, Mazur A, Amouroux P, Rémésy C. 2004. Health effect of vegetable-based diet: lettuce consumption improves cholesterol metabolism and antioxidant status in the rat. Clin. Nutr. 23, 605-614. doi:10.1016/j.clnu.2003.10.009 PMid:15297097

Olshansky SJ, Passaro DJ, Hershow RC. 2005. A potential decline in life expectancy in the United States in the 21st century. N. Engl. J. Med. 352, 1138-1145. doi:10.1056/NEJMsr043743 PMid:15784668

Pace-Asciak CR, Hahn S, Diamandis EP, Soleas G, Goldberg DM. 1995. The red wine phenolics transresveratrol and quercetin block human platelet aggregation and eicosanoid synthesis: Implications for protection against coronary heart disease. Clin. Chim. Acta 237, 207-219. doi:10.1016/0009-8981(95)06045-1

Pellegrini N, Serafini M, Colombi B, Del Rio D, Salvatore S, Bianchi M. 2003. Total antioxidant capacity of plant foods, beverages, and oils consumed in Italy assessed by three different in vitro assays. J. Nutr. 133, 2812-2819. PMid:12949370

Poli A, Marangoni F, Paoletti R, Mannarino E, Graziana Lupattelli B, Notarbartolo A, Aureli P, Bernini F, Cicero A, Gaddi A, Catapano A, Cricelli C, Gattone M, Marrocco W, Porrini M, Stella R, Vanotti A Volpe M. 2008. Non-pharmacological control of plasma cholesterol levels. Nutrition, Metabolism and Cardiovascular Diseases 18, S1-S16. doi:10.1016/j.numecd.2007.10.004 PMid:18258418

Ramirez TMA, Carrera A, Zambrana M. 2000. High incidence of hyperestrogenemia and dyslipidemia in a group of infertile men. Gynecol Obstet Mex 68, 224–9.

Reitman S, Frankel S. 1957 Colorimetric methods for aspartate and alanine aminotransferase. Am. J. Clin. Path. 28, 55-60.

Rosano GM, Cornoldi A, Fini M. 2005. Effects of androgens on the cardiovascular system. 28, 32-8.

Rosano GM, Sheiban I, Massaro R, Pagnotta P, Marazzi G, Vitale C, Mercuro G, Volterrani M, Aversa A, Fini M. 2007. Low testosterone levels are associated with coronary artery disease in male patients with angina. Int. J. Impot Res. 19,176-82. doi:10.1038/sj.ijir.3901504 PMid:16943795

Rustan I., Damiano M.M., Lergards G. 1993. Mise au point de la recherche d’antioxydants a usage alimentaire et applications, Falsif. Expert. Chim. Toxicol. 86, 201.

Saad F, Gooren L. 2009. The role of testosterone in the metabolic syndrome: a review. J. Steroid Biochem Mol. Biol. 114, 40-3. doi:10.1016/j.jsbmb.2008.12.022 PMid:19444934

Saito M, Yamauch Y. 1990. Isolation of tocopherols from wheat germ oil by recycle semi preparative supercritical fluid chromatography. J. Chromatogr. 505, 257-271. doi:10.1016/S0021-9673(01)93084-5

Saito M, Ikushima Y, Hatakeda K, Ito S, GotoT. 1993. Fractional extraction of rice bran oil and its esters with supercritical carbodioxide. Int. Chem. Eng. 33, 307–314.




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