A CLA enriched diet improves organ damage associated with the metabolic syndrome in spontaneous hypertensive rats
DOI:
https://doi.org/10.3989/gya.033410Keywords:
Conjugated linoleic acid, Endothelial dysfunction, Insulin resistance, Metabolic syndrome, SteatosisAbstract
The purpose of this study was to provide evidence that dietary CLA can prevent the pathogenesis of metabolic syndrome in tissue structure, suggesting potential benefits in the onset of this syndrome. Wistar male spontaneous hypertensive rats (SHR), were classified into two groups that were fed a standard diet for eight weeks: one with 7.5% sunflower oil (V-SHR group), and the other with 6% sunflower oil and 1.5% CLA (CLA-SHR group). A control healthy group consisted of Kyoto-Wistar male rats fed the standard diet with 7.5% sunflower oil. The animals were sacrificed, and sections of liver, kidneys and aorta were fixed in 10% formaldehyde, and then stained with Hematoxylin and Eosin. Only in the V-SHR group, the stain of the aorta indicated irregular endothelial morphology; liver parenchyma was characterized by an infiltration of inflammatory neutrophils, fibrosis, thickening of the portal vein epithelium, hepatocyte hyperplasia and steatosis. The renal tissue of this group evidenced hyperplasia in the cells of the endothelial of Bowman´s capsule. Abnormal histological changes were not observed in either the control group or the rats fed with CLA, suggesting a protective role of CLA in the onset of metabolic syndrome.
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Alexander A, Hernandez G, Lara M, Angulo JO, Oliart RM. 2004. Effects of fish oil on hypertension, plasma lipids and tumor necrosis factor alpha (TNF-α) in rats with sucrose-induced metabolic syndrome. J. Nutr. Biochem. 15, 350-357. doi:10.1016/j.jnutbio.2003.12.008 PMid:15157941
Aljada A, Ghanim H, Mohanty P, Kapur N, Dandona P. 2002. Insulin inhibits the pro-inflammatory transcription factor early growth response gene-1 8Egr9-1 expression in mononuclear cells (MNC) and reduces plasma tissue factor (TF) and plasminogen activator inhibitor-1 (PAI-1) concentrations. J. Clin. Endocrinol. Metab. 87, 1419-1422. doi:10.1210/jc.87.3.1419
Assmann G. 1979. Current diagnosis of hyperlipidemias. Internist Berl. 20, 559-564.
Barham D, Trinder P. 1972. An improved color reagent for the determination of blood glucose by the oxidase system. Analyst 97, 142–145. doi:10.1039/an9729700142 PMid:5037807
Bayindir O, Özmen D, Mutaf I, Turgan N, Habif S, Gülter C, Parildar Z, Uysal A. 2002. Comparision of the effects of dietary saturated, mono-, and n-6 polyunsaturated fatty acids on blood lipid profile, oxidant stress, prostanoid synthesis and aortic histology in rabbits. Ann. Nutr. Metab. 46, 222-228. doi:10.1159/000065411 PMid:12378047
Bhattacharya A, Banu J, Rahman M, Causey J, Fernandes G. 2006. Biological effects of conjugated linoleic acids in health and disease. J. Nutr. Biochem. 17, 789-810. doi:10.1016/j.jnutbio.2006.02.009 PMid:16650752
Bayliss HOB. 1990. Lipids in: Theory and Practice of Histological Techniques, Bancroft JD, Stevens A (Eds.), Edinburgh, Churchill Livingstone, 215-244.
Belury MA, Moya-Camarena SY, Shi L, Leesnitzer LM, Blanchard SG. 2002. Conjugated linoleic acid is an activator and ligand for peroxisome proliferator-activated receptor-gamma (PPARγ). Nutr. Res. 22, 817-824. doi:10.1016/S0271-5317(02)00393-7
Cohen RA. 1995. The role of nitric oxide and other endothelium derived vasoactive substances in vascular disease. Prog. Cardio-vas Dis. 38, 105-28. doi:10.1016/S0033-0620(05)80002-7
Chen D, Mauvais-Jarvis F, Bluher M, Fisher JS, Jozsi A, Goodyear JL, Ueki K, Kahn RC. 2004. p50/p55. Phosphoinositide 3-Kinase knockout mice exhibit enhanced insulin sensitivity. Mol. Cel. Biol. 241, 320-329. doi:10.1128/MCB.24.1.320-329.2004 PMid:14673165 PMCid:303335
Chin SF, Liu W, Storkson JM, Ha YL, Pariza MW. 1992. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J. Food Comp. Anal. 5, 185-197. doi:10.1016/0889-1575(92)90037-K
Dandona P, Aljada A, Chaudhuri A, Mohanty P, Gary R. 2005. Metabolic Syndrome. A comprehensive perspective based on interactions between obesity, diabetes, and inflammation. Circulation 111, 1448-1454. doi:10.1161/01.CIR.0000158483.13093.9D PMid:15781756
Hotamisligil GS, Shargill NS, Spiegelman BM. 1993. Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science 259, 87-91. doi:10.1126/science.7678183 PMid:7678183
Isharwal S, Misra A, Wasir JS, Nigam P. 2009. Diet & insulin resistance: A review & Asian Indian perspective. Indian J. Med. Res. 129, 485-499. PMid:19675375
Kumar NS, Eapen ACh, Pullimood BA, Balasubramanian AK. 2006. Oxidative Stress in experimental liver microvesicular steatosis: Role of mitochondria and peroxisomes. J. Gastr. Hepat. 21, 1240-1249. doi:10.1111/j.1440-1746.2006.04313.x PMid:16872304
Karninski KA, Bonda TA, Korechi J, Musial W. 2002. Oxidative stress and neutrophil activation. The two keystones of ischemia/reperfusion injury. Int. J. Cardiol. 86, 41-59. doi:10.1016/S0167-5273(02)00189-4
Lamarche B, Desroches S. 2006. Metabolic syndrome and effects of conjugated linoleic acid in obesity and lipoprotein disorders: the Québec experience. Am. J. Clin. Nutr. 79, 1149S-1152S.
Lee KN, Kritchevsky D, Pariza MW. 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108, 19-25. doi:10.1016/0021-9150(94)90034-5
Moloney F, Toomey S, Noone E, Nugent A, Allan B, Loscher ECh, Roche MH. 2007a. Antidiabetic Effects of cis-9, trans-11–conjugated linoleic acid may be mediated via anti-inflammatory effects in white adipose tissue. Diabetes 56, 574-582. doi:10.2337/db06-0384 PMid:17327424
Moloney F, Toh-Peng YTP, Mullen A, Nolan JJ, Roche MH. 2007b. Conjugated linoleic acid supplementation, insulin sensitivity, and lipoprotein metabolism in patients with type 2 diabetes mellitus. Am. J. Clin. Nutr. 80, 887-895.
Mullen A, Moloney F, Nugenet PA, Doyle L, Cashman DK, Roche MH. 2007. Conjugated linoleic acid supplementation reduces peripheral blood mononuclear cell interleukin-2 production in healthy middle-aged males. J. Nutr. Biochem. 18, 658-666. doi:10.1016/j.jnutbio.2006.12.008 PMid:17368881
Nagao K, Inoue N, Wang YM, Yanagita T. 2003. Conjugated linoleic acid enhances plasma adiponectin level and alleviates hyperinsulinemia and hypertension in Zucker diabetic fatty (fa/fa) rats. Biochem. Biophys. Res. Commun. 310, 562-566. doi:10.1016/j.bbrc.2003.09.044 PMid:14521947
National Research Council. Guide for the Care and Use of Laboratory Animals.1985. Publication no. 85- 23 (revised). Bethesda, MD: National Institutes of Health.
Nicolosi RJ, Rogers EJ, Kritchevsky D, Scimeca JA, Huth PJ. 1997. Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic atherosclerosis in hypercholesterolemic hamsters. Artery 22, 266-277. PMid:9209699
Ohnuki K, Haramizu S, Ishihara K, Fushiki T. 2001. Increased energy metabolism and suppressed body fat accumulation in mice by low concentration of conjugated linoleic acid. Biosci. Biotechnol. Biochem. 65, 2200-2204. doi:10.1271/bbb.65.2200
Phillips MC, Tierney CA, Roche MH. 2008. Gene-Nutrient interactions in the metabolic syndrome. J. Nutrigen. Nutrigenom. 1, 136-151. doi:10.1159/000112461 PMid:19776623
Reaven GM. 1998. Role of insulin resistance in human disease. Diabetes 37, 1595-1607. doi:10.2337/diabetes.37.12.1595 PMid:3056758
Reaven GM. 2002. Metabolic syndrome: patophysiology and implications for management of cardiovascular disease. Circulation 106, 286-288. doi:10.1161/01.CIR.0000019884.36724.D9 PMid:12119239
Roche MH. 2005. Fatty acids and metabolic syndrome. Proc. Nutr. Soc. 64, 23-29. doi:10.1079/PNS2004405 PMid:15877919
Rodríguez E, Perea JM, López-Sobaler AM, Ortega RM. 2009. Obesidad, resistencia a la insulina y aumento de los niveles de adipoquinas: importancia de la dieta y el ejercicio físico. Nutr. Hosp. 24, 415-42I.
Terpstra AHM. 2004. Effect of conjugated linoleic acid on body composition and plasma lipids in humans: an overview of the literature. Am. J. Clin. Nutr. 79, 352-361. PMid:14985207
Tricon S, Burdge CG, Jones LE, Russell JJ, El-Khazen S, Moretti E. 2006. Effects of dairy products naturally enriched with cis-9, trans-11 conjugated linoleic acid on the blood lipid profile in healthy middle-aged men. Am. J. Clin. Nutr. 83, 744-753. PMid:16600923
Wang YW, Jones PJH. 2004. Dietary Conjugated linoleic acid and body composition. Am. J. Clin. Nutr. 79, 1153S-1158S. PMid:15159250
Weir GC & Bonner WS. 2007. A dominant role for glucose in β cell compensation of insulin resistance. J. Clin. Invest. 117, 81-83. doi:10.1172/JCI30862 PMid:17200709 PMCid:1716221
Wilson AT, Kritchevsky D, Kotyla T, Nicolosi JR. 2006. Structured triglycerides containing caprylic (8:0) and oleic (18:1) fatty acids reduce blood cholesterol concentrations and aortic cholesterol accumulation in hamsters. Biochim. Biophys. Acta 1761, 345-349. PMid:16580874
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