Antioxidant, anti-inflammatory and cytotoxic activities of the unsaponifiable fraction of extra virgin olive oil
DOI:
https://doi.org/10.3989/gya.0916192Keywords:
Anti-inflammatory, Antioxidant, Cytotoxicity, Olive oil, Unsaponifiable fractionAbstract
The health benefits of olive oil are well-known. In this study, the unsaponifiable fraction of extra virgin olive oil (Unsap) was investigated for reducing power capacity, ferric reducing antioxidant power, fer-rous chelating activity and nitric oxide inhibition. The present study was also designed to evaluate the in vitro cytotoxic effect of the Unsap against human carcinoma cells. The anti-inflammatory potential of Unsap has been determined via the inhibition of Human Cyclooxygenases. The results showed that Unsap is efficient for ferric reducing antioxidant power and nitric oxide inhibition. Unsap has a selective effect as anti-inflammatory agent. The results showed moderate to good in vitro antitumor activities of Unsap against human liver, lung and pancreas cancer cells with IC50 ranging from 19.6 to 30.4 μg/mL and good selectivity index (≥ 2). In conclusion, Unsap represents a promising and safe antitumor and antioxidant material that supports the need for further investigation.
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Abdallah M, Marzocco S, Adesso S, Zarrouk M, Guerfel M. 2018. Olive oil polyphenols extracts inhibit inflammatory markers in J774A.1 murine macrophages and scavenge free radicals. Acta Histochem. 120, 1-10. https://doi.org/10.1016/j.acthis.2017.10.005 PMid:29128095
Abraham A, Kattoor AJ, Saldeen T, Mehta JL. 2019. Vitamin E and its anticancer effects. Crit. Rev. Food Sci. 59, 2831-2838. https://doi.org/10.1080/10408398.2018.1474169 PMid:29746786
Atere TG, Akinloye OA, Ugbaja RN, Ojo DA, Dealtry G. 2018. In vitro antioxidant capacity and free radical scavenging evaluation of standardized extract of Costus afer leaf. Food Science and Human Wellness 7, 266-272. https://doi.org/10.1016/j.fshw.2018.09.004
Boskou D. 2015. Olive and Olive Oil Bioactive Ingredients. AOCS Press, Urbana, IL, USA. https://doi.org/10.1016/B978-1-78242-376-8.00001-6
Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. 2018. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA-Cancer J. Clin. 68, 394-424. https://doi.org/10.3322/caac.21492 PMid:30207593
Canabady-Rochelle LL, Harscoat-Schiavo C, Kessler V, Aymes A, Fournier F, Girardet JM. 2015. Determination of reducing power and metal chelating ability of antioxidant peptides: revisited methods. Food Chem. 15, 129-35. https://doi.org/10.1016/j.foodchem.2015.02.147 PMid:25863620
Cardeno A, Magnusson MK, Strid H, Alarcón de La Lastra C, Sánchez-Hidalgo M, Ohman L. 2014. The unsaponifiable fraction of extra virgin olive oil promotes apoptosis and attenuates activation and homing properties of T cells from patients with inflammatory bowel disease. Food Chem. 161, 353-360. https://doi.org/10.1016/j.foodchem.2014.04.016 PMid:24837962
Cardeno A, Sánchez-Hidalgo M, Cortes-Delgado A, de la Lastra CA. 2013. Mechanisms involved in the antiproliferative and proapoptotic effects of unsaponifiable fraction of extra virgin olive oil on HT-29 cancer cells. Nutr. Cancer. 65, 908-918. https://doi.org/10.1080/01635581.2013.806674 PMid:23909736
Codex Alimentarius Commission 2015. Codex Standard for Named Standard for Edible Fats and Oils. Codex Stan 210-1999.
Covas MI, Konstantinidou V, Fitó M. 2009. Olive oil and cardiovascular health. J. Cardiovasc. Pharmacol. 54, 477-482. https://doi.org/10.1097/FJC.0b013e3181c5e7fd PMid:19858733
De Stefanis D, Scimè S, Accomazzo S, Catti A, Occhipinti A, Bertea CM, Costelli P. 2019. Anti-proliferative effects of an extra-virgin olive oil extract enriched in ligstroside aglycone and oleocanthal on human liver cancer cell lines. Cancers 11, 1640. https://doi.org/10.3390/cancers11111640 PMid:31653043 PMCid:PMC6896128
Escrich E, Moral R, Solanas M. 2011. Olive oil, an essential component of the Mediterranean diet, and breast cancer. Public Health Nutr. 14, 2323-2332. https://doi.org/10.1017/S1368980011002588 PMid:22166191
Franco MN, Galeano-Diaz T, López O, Fernández-Bolanos JG, Sánchez J, De Miguel C, Gil, MV, Martin-Vertedor D. 2014. Phenolic compounds and antioxidant capacity of virgin olive oil. Food Chem. 163, 289-298. https://doi.org/10.1016/j.foodchem.2014.04.091 PMid:24912728
Galal SM, Morsi MKS, Abd El-Rahman MK, Darwish SK, Katry MA. 2020. Hepatoprotective effect of unsaponifiable matter from olive, linseed and sesame oils against carbon tetrachloride-induced liver injury in rats. Grasas Aceites 71 (1), e345. https://doi.org/10.3989/gya.1175182
Gülҫin I, Buyukokuroglu ME, Kufrevioglu OI. 2003. Metal chelating and hydrogen peroxide scavenging effects of melatonin. J. Pineal Res. 34, 278-281. https://doi.org/10.1034/j.1600-079X.2003.00042.x PMid:12662350
International Union of Pure and Applied Chemistry (IUPAC). 1992. Standard Methods for the Analysis of Oils, Fats and Derivatives. 7th edn, Blackwell Scientific Publications, Oxford, England.
Larsen LN, Dahl E, Bremer J. 1996. Peroxidative oxidation of leuco-dichlorofluorescein by prostaglandin H synthase in prostaglandin biosynthesis from polyunsaturated fatty acids. Biochim. Biophys. Acta. 1299, 47-53. https://doi.org/10.1016/0005-2760(95)00188-3
Marcocci I, Marguire JJ, Droy-lefaiz MT, Packer L. 1994. The nitric oxide scavenging properties Ginkgo biloba extract. Biochem. Biophys. Res. Commun. 201, 748-755. https://doi.org/10.1006/bbrc.1994.1764 PMid:8003011
Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65, 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
Newmark HL. 1997. Squalene, olive oil, and cancer risk: A review and hypothesis. Cancer Epidemiol. Biomarkers Prev. 6, 1101-1103.
Oyaizu M. 1986. Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine. Japn. J. Nutr. Diet. 44, 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
Park SW, Lee SG, Song SH, Heo DS, Park BJ, Lee DW, Kim KH, Sung MW. 2003. The effect of nitric oxide on cyclooxygenase-2 (COX-2) overexpression in head and neck cancer cell lines. Int. J. Cancer. 107, 729-738. https://doi.org/10.1002/ijc.11498 PMid:14566822
Rao CV, Newmark HL, Reddy BS. 1998. Chemopreventive effect of squalene on colon cancer. Carcinogenesis 19, 287-290. https://doi.org/10.1093/carcin/19.2.287 PMid:9498278
Sánchez-Fidalgo S, Cárdeno A, Sánchez-Hidalgo M, Aparicio- Soto M, Villegas I, Rosillo MA, de la Lastra CA. 2013. Dietary unsaponifiable fraction from extra virgin olive oil supplementation attenuates acute ulcerative colitis in mice. Eur. J. Pharm. Sci. 48, 572-581. https://doi.org/10.1016/j.ejps.2012.12.004 PMid:23238173
Servili M, Sordini B, Esposto S, Urbani S, Veneziani G, Di Maio I, Selvaggini R, Taticchi, A. 2014. Biological activities of phenolic compounds of extra virgin olive oil. Antioxidants 3, 1-23. https://doi.org/10.3390/antiox3010001 PMid:26784660 PMCid:PMC4665453
Smith TJ, Yang GY, Seril DN, Liao J, Kim S. 1998. Inhibition of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone induced lung tumorigenesis by dietary olive oil and squalene. Carcinogenesis 19, 703-706. https://doi.org/10.1093/carcin/19.4.703 PMid:9600360
Solanas M, Grau L, Moral R, Vela E, Escrich R, Escrich E. 2010. Dietary olive oil and corn oil differentially affect experimental breast cancer through distinct modulation of the p21Ras signaling and the proliferation-apoptosis balance. Carcinogenesis 31, 871-879. https://doi.org/10.1093/carcin/bgp243 PMid:19825967
Toscano R, Millán-Linares M C, Naranjo M C, Lemus-Conejo A, Claro C, Montserrat-de la Paz S. 2019. Unsaponifiable and phenolic fractions from virgin olive oil prevent neuroinflammation skewing microglia polarization toward M2 phenotype. J. Funct. Foods 62, 103543. https://doi.org/10.1016/j.jff.2019.103543
Valderrama JA, Delgado V, Sepúlveda S, Benites J, Theoduloz C, Buc Calderon P, Muccioli GG. 2016. Synthesis and cytotoxic activity on human cancer cells of novel isoquinoline quinone-amino acid derivatives. Molecules 21, 1-14. https://doi.org/10.3390/molecules21091199 PMid:27617997 PMCid:PMC6274474
Warleta F, Campos M, Allouche Y, Sánchez-Quesada C, Ruiz- Mora J, Beltrán G, Gaforio JJ. 2010. Squalene protects against oxidative DNA damage in MCF10A human mammary epithelial cells but not in MCF7 and MDA-MB-231 human breast cancer cells. Food Chem. Toxicol. 48, 1092-1100. https://doi.org/10.1016/j.fct.2010.01.031 PMid:20138105
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