Enzymatic treatment on oil extraction and antioxidant recuperation from Oenothera biennis by cold pressing.

Authors

  • César A. Collao Instituto de Química Pontificia Universidad Católica de Valparaíso
  • Emilia Curotto Instituto de Química Pontificia Universidad Católica de Valparaíso
  • María E. Zúñiga Escuela de Ingeniería Bioquímica

DOI:

https://doi.org/10.3989/gya.2007.v58.i1.2

Keywords:

Antioxidants, Enzymes, Evening primrose, Oenothera biennis oil, Oil extraction, Polyphenol

Abstract


The aim of this work was to improve the yield in evening primrose oil extraction and the antioxidant potential of the residual meal using an enzyme assisted extraction process with cold pressing. Among four commercial enzymes, a mixture of two of them was selected in order to evaluate the effect of enzyme-substrate ratio, temperature, moisture and period of time of the enzymatic pretreatment on the extracted oil. The oil yield increased by 12% when the seeds were pretreated with 2% enzyme-substrate ratio of a mixture of 100G Ultrazym and Cellubrix (1:1) for 15 h at 40oC and 40% moisture. The defatted meal obtained from the enzyme aided process enhanced its phenol content and improved its potential antioxidant activity in comparison to the control from 250 to 276 mg of phenols/g of meal and from 1463 to 1558 mg of trolox/g of meal respectively.

Downloads

Download data is not yet available.

References

Association of Official Analytical Chemists (AOAC) 1990. Official methods of analysis, 15th ed. 776-782, Arlington, EEUU.

Balasinska B, Troszynska A. 1998. Total antioxidative of Evening Primrose (Oenothera paradox) cake extract measure in vitro by liposome model and murine L1210 cells. J Agric Food Chem. 46, 3558-3563. doi:10.1021/jf980193y

Brand WW, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. Lebensm Wiss Technol. 28, 25-30.

Chamy R, Domínguez H, Franco D, Lema JM, López- Munguía A, Moure A, Núñez MJ, Santamaría RI, Sineiro J, Soto C, Zúñiga ME. 2001. Enzyme-aided alternative processes for the extraction of oil from Rosa rubiginosa. J Am Oil Chem Soc. 78, 437-439. doi:10.1007/s11746-001-0281-4

Concha J, Soto C, Chamy R, Zúñiga ME. 2004. Effect of Enzymatic Pretreatment on Rose Hip Oil Extraction: Hydrolysis and Pressing Conditions. J Am Oil Chem Soc. 81(6), 550-552. doi:10.1007/s11746-006-0939-y

Cruz JM, Domínguez JM, Domínguez H, Parajó JC. 1999. Solvent extraction of hemicellulosic wood hydrolysates, a procedure useful for obtaining both detoxified fermentation media and polyphenols with antioxidant activity. Food Chem. 67, 147–153. doi:10.1016/S0308-8146(99)00106-5

Guerra EG, Zúñiga ME. 2003. Tratamiento enzimático en la extracción de aceite de pepa de uva, Vitis vinifera, por prensado en frío. Grasas y Aceites 54, 53-57.

Harbone JB, Williams CA. 2000. Review: Advances in Flavonoid research since 1992. Phytochem. 55, 481-504. doi:10.1016/S0031-9422(00)00235-1

Hoffmann G. 1989 The chemistry and technology of edible oils and fats and their high fat products”. Food Sci Technol. A series of monographs: 29-137. Academia Press Inc. London.

Hudson, BJF. 1984. Evening primrose (Oenthera spp.) oil and seed. J Am Oil Chem Soc. 61(3), 540-543. doi:10.1007/BF02677026

Instituto Nacional de Normalización (INN) 1988. Nch 485. Granos o semillas oleaginosas: Determinación del extracto al éter de petróleo denominado contenido de aceite.

Landbo AK, Meyer AS. 2001. Enzyme-assisted extraction of antioxidative phenols from black current juice press residues (Ribes nigrum). J Agric Food Chem. 49, 3169-3177. doi:10.1021/jf001443p

Mätthaus B. 2002. Antioxidant activity of extracts obtained from residues of different oilseed. J Agric Food Chem. 50, 3444-3452. doi:10.1021/jf011440s

Meyer AS, Jepsen SM, Sørensen NS. 1998. Enzymatic release of antioxidants for human low-density lipoprotein from grape pomace. J Agric Food Chem. 46, 2439–2446. doi:10.1021/jf971012f

Miller GL. 1959. Use of Dinitrosalicylic Acid: Reagent for Determination of reducing Sugar. Anal Chem. 31, 426-428. doi:10.1021/ac60147a030

Moure A, Domínguez H, Zúñiga ME, Soto C, Chamy R. 2002. Characterization of protein concentrates from Guevina avellana pressing cakes. Food Chem. 78, 179-186. doi:10.1016/S0308-8146(01)00397-1

Norris FA. 1982. Extraction of fats and oils. In: Swern, D. (ed.) Bailey’s industrial oil and fat products. 2, 178-253, John Wiley and Sons Inc., Canadá.

Santamaría RI, Soto C, Zúñiga ME, Chamy R, López-Munguía A. 2003. Enzymatic extraction of oil from gevuina avellana, the chilean hazelnut. J Am Oil Chem. Soc. 80 (1), 33-36. doi:10.1007/s11746-003-0646-8

Singleton VL and Rossi JA Jr. 1965. Colorimetry of Total Phenolics with Phosphomolybdic phosphotungstic Acid Reagents. Am J Enology and Viticulture 16, 144- 158.

Soto C, Chamy R, Zúñiga ME. 2004. Effect of enzymatic application on borage (Borago officinalis) oil extraction by cold pressing. J Chem Eng of Japan 37, 326-331. doi:10.1252/jcej.37.326

Spiegel M. Estadística. Mc Graw Hill, Interamericana de España, S.A. 1991, pp 375-410.

Tobar P, Moure A, Soto C, Chamy R, Zúñiga M E 2005. Winery Solid Residue Revalorization into Oil and Antioxidant with Nutraceutical Properties by an Enzyme Assisted Process. J Water Sci Technol. 517, 47-52.

Van Soest PJ, Wine RH. 1968. Determination of Lignin and Cellulose in Acid-Detergent Fiber with Permanganate, Journal of the A.O.A.C. 51, 780-785.

Van Soest PJ, Robertson JB, Lewis BA. 1991. Methods for Dietary Fiber, Neutral Detergent Fiber, and Non Starch Polysaccharides in Relation to Animal Nutrition, J. Dairy Sci. 74, 3583-3597.

Wettasinghe M, Shahidi F. 1999. Evening Primrose Meal: A source of natural antioxidants and scavenger of hydrogen peroxide and oxygen-derived free radicals. J. Agric Food Chem. 47, 1801-1812. doi:10.1021/jf9810416

Zuñiga ME, Soto C, Mora A, Chamy R, Lema JM. 2003. Enzymic pre-treatment of Guevina avellana mol oil extraction by pressing. Process Biochem. 39, 51-57. doi:10.1016/S0032-9592(02)00286-8

Downloads

Published

2007-03-30

How to Cite

1.
Collao CA, Curotto E, Zúñiga ME. Enzymatic treatment on oil extraction and antioxidant recuperation from Oenothera biennis by cold pressing. Grasas aceites [Internet]. 2007Mar.30 [cited 2024Mar.29];58(1):10-4. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/2

Issue

Section

Research