GC-MS study of Artabotrys odoratissimus fatty oil ( leaves )

El estudio por GC-MS de dos fracciones del aceite de la h o j a d e Artabotrys odoratissimus ind icó la presencia de quince compuestos tales como: ácido nonanoico; fenil propanoato de metilo; ácido decanoico; ftalato de dietilo; ftalato de dibutilo; 2-amino-3-etil bifenilo; 5-metil-9-fenilnonan-3-ol; hexadeca-2,7,11-trieno; 2,6-dimetil-1-fenilheptan-1-ona; 2,5-dimetiltetradecahidrofenantreno; 1-fenilundecano; 1-isopropil-4,6-dimetil naftaleno; 5-(2-butilfenil)-3-penten-2-ol; 1-fenildecan-1-ona y 1-fenilundecan-1-ona. Algunos de estos compuestos son poco frecuentes y activos biológicamente.


INTRODUCTION
Artabotrys odoratissimus locally known as "Kantili champa" is an ornamental plant and belongs to family Annonaceae.Its flowers are used in the treatment of vomiting, biliousness, blood and heart disease, itching, sweating, foul breath, thirst and headache.Decoction of its leaves are given for the treatment of cholera (1,2).
The antifertility activity of A. odoratissimus has been confirmed in rats (3).The essential oil of leaves of A. odoratissimus has shown excellent to good antihelmintic property against tape worms, earthworms and round worms (4).From the stem bark of A. odoratissimus two steroids, β-sitosterol and stigmasterol, an aromatic compound benzyl benzoate and a noraporphine alkaloid asimilabine were isolated (5).

EXPERIMENTAL
The leaves were collected from nearby gardens and were identified by the authorities from the School of Botany, Vikram University, Ujjain.The shade dried leaves were powdered and extracted in soxhlet extractor with n-hexane.Removal of the solvent under reduced pressure left a solid mass, which was fractionated on an alumina grade III column.The column was eluted with different solvents of increasing order of polarity.All the fractions of similar TLC pattern were mixed together and the solvent was recovered.
Hexane and hexane:benzene (9:1,v/v) fractions of this column yielded two oily fractions.Rechromatography of both fractions was done on silica gel column.Rechromatography of hexane fraction yielded oily fraction in hexane eluate (AOGM-1) and rechromatography of hexane:benzene (9:1, v/v) fraction yielded another oily fraction in 10% hexane: benzene eluate (AOGM-3).As it was difficult to separate the compounds present in the oil by column chromatography, we undertook the study of both fractions by GC-MS.
The oil was analyzed at the Regional Sophisticated Instrumentation Center, IIT-Bombay, Mumbai by GC-MS.The operating parameters were as follows: 5.5 x 4 mm id glass column, packed with carbowax 20 M (10% on chromosorb W, AW and DMCS treated); helium gas was used as a carrier gas at the rate of 40 ml/min , temp.

RESULTS
The GC-MS analysis of both fractions, AOGM-1 and AOGM-3 revealed the presence of nine and seven peaks respectively, corresponding to sixteen compounds in all , out of which fifteen compounds were identified by comparing their retention times, covet indexes and by interpretation of their mass spectra (9,10,11).The peak value of one compound was poor and hence rejected.Compounds I to VI were isolated from fraction code AOGM-3 and compounds VII to XV were isolated from fraction code AOGM-1.
The oil also contains diethyl phthalate (16.77 %) which is a toxic compound used as plasticiser (14).Decanoic acid (14 %) is also found as a glyceroids in some milks.It is used as corrosion inhibitor and surfactant.Its simple esters have perfumery and flavor properties (15,16).The results and mass fragmentation data are being given in Table I .
70 o C to 200 o C, at 4 o C/ min, detector and injection port heaters were Grasas y Aceites maintained at 300 o C and 200 o C, respectively.Significant operating parameters of the MS were: ionisation voltage 70eV; ionisation current 100 µA; source temperature 225 o C, accelerating voltage 1.33 kV; resolution 1000; scan speed 3 sec/decade.Data were processed on a Kratos DS-50 data processing system.