Influence of ionizing irradiation on the antioxidant enzymes of Vicia faba L.
Keywords:Antioxidants enzyme, Gamma irradiation, Laser irirradiation, Vicia faba L.
The seeds of Vicia faba L. Giza 834 were exposed to γ-irradiation at dose levels of 2.5, 5.0, 10.0 and 20.0 kGy and after that exposed to He–Ne (632.8 nm) or diode (650.0 nm) laser irradiation for 5 min. The activities of POD, APOX, CAT, SOD and GST enzymes were significantly stimulated and this stimulation reached its maximum at a dose level of 5.0 kGy for enzymes POD, APOX, CAT and SOD, but for GST enzyme at a dose level 10.0 kGy. For He- Ne laser, with or without different doses (2.5, 5.0, 10.0 and 20.0 kGy) of γ-irradiation, enzyme induction was significantly stimulated and positively correlated with the dose levels of γ-irradiation in combination with the He-Ne treatment. There was a significant increase in the concentration of MDA and this increase was more pronounced at dose level 20.0 kGy (38.2 μmol/g d.w) compared to the control (3.9 μmol/g d.w). Laser treatment by He–Ne laser or diode laser only caused a slight increase (P < 0.05) in MDA content (4.4 and 5.08 μmol/g d.w respectively) compared to the control (3.9 μmol/g d.w). H2O2 content significantly increased in all treatments and this increase reached its maximum at dose level 20.0 kGy (36.3 μmol/g d.w) compared to the control (2.3 μmol/g d.w.). On the other hand, He-Ne or diode laser treatments combined with γ-irradiation significantly decreased in comparison with γ-irradiation treatments alone. In the case of glutathione content, there were significant increases by γ-irradiation at dose levels 2.5, 5.0, 10.0 and 20.0 kGy. Furthermore, with a combination of γ-irradiation and He-Ne or diode laser, a marked increase in glutathione content was found and was more pronounced than that of gamma irradiation alone.
Aebi H. 1983. Catalase, in H Bergmeyer (Ed.) Methods of Enzymatic Analysis, vol 3. Verlag Chemie, Weinheim, Adamse, pp. 273-277.
Al-Kaisey MT, Mohammed MA, Alwan AKH, Mohammed, MH. 2002. The effect of gamma irirradiation on the viscosity of two barley cultivars for broiler chicks. Irradiation Physics and Chemistry 63, 295-297. doi:10.1016/S0969-806X(01)00587-4
Asada K. 1992. Ascorbate peroxidase-a hydrogen peroxide scavenging enzyme in plants. Physiologia Plantarum 85, 235– 241. doi:10.1111/j.1399-3054.1992.tb04728.x
Bari ML, Nazuka E, Sabina Y, Todoriki S, Isshiki K. 2003. Chemical and irirradiation treatments for killing Escherichia coli O157: H7 on alfalfa, radis, and mung bean seeds. Journal of Food Protection 66, 767-774. PMid:12747683
Beauchamp C, Fridovich I. 1971. Superoxide dismutase improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44, 276-287. doi:10.1016/0003-2697(71)90370-8
Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254 doi:10.1016/0003-2697(76)90527-3
Cai SW, Qi Z, Ma XL. 2000. The effect of He–Ne laser irirradiation on soluble protein synthesis of corn seedling. Chinese Journal Lasers 27, 284-288.
Cai SW, Zhao XS, Lu FT, Ma XL. 1994. The influence of He–Ne laser irirradiation on the active oxygen metabolism of corn seedlings. Chinese Journal Lasers 21, 767-769.
Cakmak I, Horst WJ. 1991. Effect of aluminum on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tips of soybean (Glycine max). Journal of Plant Physiology 83, 463-468. doi:10.1111/j.1399-3054.1991.tb00121.x
Capaldi DJ, Taylor KE. 1983. A new peroxidase color reaction: oxidative coupling of 3-methyl-2-benzothiazolinone hydrazone (MBTH) with its formaldehyde azine application to glucose and choline oxidases. Analytical Biochemistry 129, 329–336. doi:10.1016/0003-2697(83)90558-4
Chaomei Z, Yanlin M. 1993. Irradiation induced changes in enzymes of wheat during seed germination and seedling growth. Acta Agriculturae Nucleatae Sinica 7, 93-97.
Chen Y, Yuea M, Wanga X. 2005. Influence of He–Ne laser irirradiation on seeds thermodynamic parameters and seedlings growth of Isatis indogotica. Plant Science 168, 601-606. doi:10.1016/j.plantsci.2004.09.005
Cho YS, Lee HS, Pai HS. 2000. Expression patterns of diverse genes in response to gamma irirradiation in Nicotiana tabacum. Journal of Plant Biology 43, 82-87. doi:10.1007/BF03030499
Costa H, Gallego SM, Tomaro ML. 2002. Effect of UV-B irradiation on antioxidant defense system in sunflower cotyledons. Plant Science 162, 939-945. doi:10.1016/S0168-9452(02)00051-1
Cotter DJ, Sawyer RL. 1961. The effect of -irirradiation on the incidence of black spot and ascorbic acid, glutathione and tyrosinase content of potato tubers. American Potato Journal 38, 58-65. doi:10.1007/BF02862805
Foyer CH, Descourvieres P, Kunert KJ. 1994a. Protection against oxygen radicals: an important defense mechanism study in transgenic plants. Plant, Cell & Environment 17, 507–523. doi:10.1111/j.1365-3040.1994.tb00146.x
Foyer CH, Lelandais M, Kunert KJ. 1994b. Photooxidative stress in plants. Physiologia Plantarum 92, 696-717. doi:10.1111/j.1399-3054.1994.tb03042.x
Galatro A, Simontacchi M, Puntarulo S. 2001. Free radical generation and antioxidant content in chloroplasts from soybean leaves exposed to ultraviolet-B. Physiologia Plantarum 113, 564-570. doi:10.1034/j.1399-3054.2001.1130416.x
Galova Z. 1996. The effect of laser beams on the process of germinating power of winter wheat grains. Roczniki AR, Poznañ, CCCLXXXVI, ser. Rol. 49, 39-43.
Gressel J, Galun E. 1994. Genetic Controls of Photooxidant Tolerance, in Foyer CH, Mullineaux PM (Ed.) Causes of photooxidative stress and amelioration of defense systems in plant. Boca Raton, CRC Press, pp. 237–274.
Han R, Wang XL, Yue M. 2002. Influence of He–Ne laser irirradiation on the excision repair of cyclobutyl pyrimidine dimers in the wheat DNA. Chinese Science Bulletin 47, 435-438. doi:10.1360/02tb9184
Haraguchi H, Saito T, Okamura N, Yagi A. 1995. Inhibition of lipid peroxidation and superoxide generation by diterpenoids from rosmarinus offcinalis. Planta Medica 61, 333-336. doi:10.1055/s-2006-958094 PMid:7480180
Horemans N, Foyer CH, Asard H. 2000. Transport and action of ascorbate at the plant plasma membrane. Trends in Plant Science 5, 263-267. doi:10.1016/S1360-1385(00)01649-6
Horvathova J, Suhaj M, Polovka M, lasta Brezov V,·imko P. 2007. The influence of gamma-irradiation on the formation of free radicals and antioxidant status of oregano (Origanum vulgare L.). Czech Journal of Food Sciences 25, 131–143.
Joshi PN, Biswal B, Biswal NC. 1991. Effect of UV-A on aging of wheat leaves and role of phytochrome. Environmental and Experimental Botany 3, 267-276. doi:10.1016/0098-8472(91)90050-X
Kunert KJ, Foyer CH. 1994. Thiol/disulphide exchange in plants, in De Kok LJ, Stulen I, Rennenberg H, Brunhold C, Rausen W (Eds.) Sulfur Nutrition and Assimilation in Higher Plants: Regulatory, Agricultural and Environmental Aspects, SPB Academic Publishers, The Hague, pp. 132-164.
Mannervik B, Guthenberg C. 1981. Glutathione transferase (Humanplacenta). Methods in Enzymology 77, 231-235. doi:10.1016/S0076-6879(81)77030-7
Markolf HN. 1996. Laser and matter, in Markolf HN (Ed.) Laser Tissues Interactions, Springer-Verlag, Berlin, Germany, pp. 8–15.
Murphy TM. 1990. Effect of broad-band ultraviolet and visible irradiation on hydrogen peroxide formation by cultured rose cells. Physiologia Plantarum 80, 63-78. doi:10.1111/j.1399-3054.1990.tb04375.x
Nakano Y, Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiology 22, 867-880.
Noctor G, Foyer CH. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology 49, 249-279. doi:10.1146/annurev.arplant.49.1.249 PMid:15012235
Podleoeny J. 2002. Effect of laser irradiation on the biochemical changes in seeds and the accumulation of dry matter in the faba bean. International Agrophysics 16, 209-213.
Qi Z, Liu X, Tian X, Yue M. 2008. Effects of CO2 laser pretreatment on drought stress resistance in wheat. Journal of Photochemistry and Photobiology 90, 17-25.
Qi Z, Yue M, Han R. 2002. The damage repair role of He–Ne laser on plants exposed to different intensities of UV-B irradiation. Journal of Photochemistry and Photobiology 75, 680-686. doi:10.1562/0031-8655(2002)075<0680:TDRROH>2.0.CO;2
Qi Z, Yue M, Wang XL. 2000. Laser pretreatment protects cells of broad bean from UV-B irradiation damage. Journal of Photochemistry and Photobiology 59, 33–37. doi:10.1016/S1011-1344(00)00131-7
Silber R, Farber M, Papopoulos E, Nervla D, Liebes L, Bruch M, Bron R. 1992. Glutathione depletion in chronic lymphocytic leukemia £]-lymphocytes. Blood 80, 2038-2040. PMid:1356514
Singh RK, Chandra P, Singh J, Singh DN. 1993. Effect of gamma-ray on Physio-biochemical parameters of sugar cane. Journal of Nuclear Agriculture & Biology 22, 65-69.
Slooten L, Capiau K, Van Camp W, Van Montagu M, Sybesma C, Inze D. 1995. Factors affecting the enhancement of oxidative stress tolerance in transgenic tobacco over expressing manganese superoxide dismutase in the chloroplasts. Plant Physiology 107, 373-380.
Smirnoff N. 1993. The role of active oxygen in the response of plants to water deficit and desiccation. New Phytologist 125, 27–58. doi:10.1111/j.1469-8137.1993.tb03863.x
Štajner D, Milosevic M, Popovic BM. 2007. Irradiation effects on phenolic Content, Lipid and protein oxidation and scavenger ability of soybean seeds. International Journal of Molecular Sciences 8, 618-627. doi:10.3390/i8070618
Štajner D, Popovic BM, Taoki K. 2009. Effects of £^-irradiation on antioxidant activity in soybean seeds. Central Journal of Biology 4, 381–386. doi:10.2478/s11535-009-0019-z
Vitória AP, Lea PJ, Azevedo RA. 2001. Antioxidant enzymes responses to cadmium in radish tissues. Phytochimstry 57, 701-710. doi:10.1016/S0031-9422(01)00130-3
Wada H, Koshiba T, Matsui T, Sato M. 1998. Involvement of peroxidase in differential sensitivity to irradiation in seedlings of two Nicotiana species. Plant Science 132, 109-119. doi:10.1016/S0168-9452(98)00005-3
Wi SG, Chung BY, Kim J, Kim J, Baek M, Lee J, Kim YS. 2007. Effects of gamma irradiation on morphological changes and biological responses in plants. Micron 38, 553-564. doi:10.1016/j.micron.2006.11.002 PMid:17157025
Willekens H, Chamnongpol S, Schraudner M, Langebartels C, Van Montagu M, Inze D, Van Camp W. 1997. Catalase is a sink for H2O2 and is indispensable for stress defense in C3 plants. EMBO Journal 16, 4806-4816. doi:10.1093/emboj/16.16.4806 PMid:9305623 PMCid:1170116
Xiong Q, Xing Z, Feng Z, Tan Q, Bian Y. 2009. Effect of 60Co £^-irradiation on postharvest quality and selected enzyme activities of Pleurotus nebrodensis. LWT - Food Science and Technology 42, 157-161.
How to Cite
Copyright (c) 2010 Consejo Superior de Investigaciones Científicas (CSIC)
This work is licensed under a Creative Commons Attribution 4.0 International License.© CSIC. Manuscripts published in both the printed and online versions of this Journal are the property of Consejo Superior de Investigaciones Científicas, and quoting this source is a requirement for any partial or full reproduction.
All contents of this electronic edition, except where otherwise noted, are distributed under a “Creative Commons Attribution 4.0 International” (CC BY 4.0) License. You may read here the basic information and the legal text of the license. The indication of the CC BY 4.0 License must be expressly stated in this way when necessary.
Self-archiving in repositories, personal webpages or similar, of any version other than the published by the Editor, is not allowed.