Chemical composition, antioxidant and antimicrobial activity of the essential oil and methanol extract of the Egyptian lemongrass Cymbopogon proximus Stapf
Keywords:Antimicrobial, Antioxidant, Chemical composition, Cymbopogon Proximus, Eo
The present study was conducted to evaluate the in vitro antimicrobial and antioxidant properties of the essential oil (Eo) and methanol extract from a unique, Egyptian endemic plant, Cymbopogon proximus STAPF. The chemical composition of a hydrodistilled Eo of C. proximus was analyzed by a GC and GC/MS system. A total of 19 constituents representing 95.47% of the oil were identified: piperitone (72.44%), elemol (9.43%), α - eudesmol (4.34%), limonene (2.45%) and β- eudesmol (1.26%) were the main components comprising 88.92% of the oil. The antimicrobial test results showed that the Eo of C. proximus strongly inhibited the growth of the test bacteria studied, except for yeast species while the methanol extract had moderate antibacterial, but no anti-candida activity. Bacillus cereus and Salmonella choleraesuis were proven to be the most susceptible against Eo. The exposure time of Eo for complete inhibition of cell viability of B. cereus and S. choleraesuis were found to be 5 % at 10 min and 1% at 10 min, respectively. The antioxidative potential of the samples was evaluated using methods of inhibition of the free radical 2,2-diphenyl-1- picrylhydrazyl (DPPH) system. The methanol extract was able to reduce the stable free radical DPPH with an IC50 of 48.66±3.1 μg/ml. The results presented here may suggest that the Eo and extracts of C. proximus possess antimicrobial and antioxidant properties, and therefore, can be used as natural preservative ingredients in food and/or pharmaceuticals.
Adams RP. 2001. Quadrupole mass spectra of compounds listed in order of their retention time on DB-5. Identification of essential oils components by gas chromatography/quadrupole mass spectroscopy. Allured Publishing Corporation, Carol. Stream, IL, USA, p. 456.
Alessandra LO, Roberta BL, Fernando AC, Marcos NE. 2005. Volatile compounds from pitanga fruit (Eugenia uniflora L.). Food Chem. 99, 1–5.
Angioni A, Barra A, Coroneo V, Dessi S, Cabras P. 2006. Chemical composition, seasonal variability, and antifungal activity of Lavandula stoechas L. ssp. stoechas essential oils from stem/leaves and flowers. J. Agric. Food Chem. 54, 4364–4370. doi:10.1021/jf0603329 PMid:16756368
Appendini P, Hotchkiss JH. 2002. Review of antimicrobial food packaging. Innovative Food Science and Emerging Technologies 3, 113−126. doi:10.1016/S1466-8564(02)00012-7
Archana B, Dasgupta N, De B. 2005. In vitro study of antioxidant activity of Syzygium cumini fruit. Food Chem. 90, 727–733. doi:10.1016/j.foodchem.2004.04.033
Borchers AT, Keen CL, Gerstiwin ME. 2004. Mushrooms, tumors, and immunity: an update. Exp. Biol. Med. 229, 393–406.
Boulos L. 1983. Medicinal Plants of North Africa. Reference Publication Inc.: Michigan, 92.
Cosentino S, Tuberoso CIG, Pisano B, Satta MV, Arzedi E, Palmas F. 1999. In vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils. Lett. Appl. Microbiol. 29, 130–135. doi:10.1046/j.1472-765X.1999.00605.x PMid:10499301
Daferera DJ, Ziogas BN, Polissiou MG. 2003. The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. Michiganensis. Crop Protection 22, 39−44. doi:10.1016/S0261-2194(02)00095-9
Djoukeng JD, Mansour EA, Tabacchi R, Tapondjou AL, Bouda H, Lontsi D. 2005. Antibacterial triterpenes from Syzygium guineense (Myrtaceae). J. Ethnopharmacol. 101, 283–286. doi:10.1016/j.jep.2005.05.008 PMid:15967609
El-Askary HI, Meselhy MR, Galal AM. 2003. Sesquiterpenes from Cymbopogon proximus. Molecules 8, 670-677. doi:10.3390/80900670
El Tahir KEH, Abdel Kader MS. 2008. Chemical and Pharmacological Study of Cymbopogon proximus Volatile Oil. Research Journal of Medicinal Plant 2 (2), 53-60. doi:10.3923/rjmp.2008.53.60
Hammer KA, Carson CF, Riley TV. 1999. Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology 86, 985−990. doi:10.1046/j.1365-2672.1999.00780.x PMid:10438227
Leimanna FV, Gonçalvesb OH, Machadoa RAF, Bolzan A. 2009. Antimicrobial activity of microencapsulated lemongrass essential oil and the effect of experimental parameters on microcapsules size and morphology. Materials Science and Engineering 29 (2), 430-436. doi:10.1016/j.msec.2008.08.025
NCCLS (National Committee for Clinical Laboratory Standards) 2000. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved Standard, M7-A5.
Onawunmi GO. 1980. Evaluation of the antifungal activity of lemongrass oil. Pharmaceutical Biology 27, 121-126. doi:10.3109/13880208909053950
Pereira RS, Sumita TC, Furlan MR, Jorge AOC, Ueno M. 2004. Antibacterial activity of essential oils on microorganisms isolated from urinary tract infection. Rev. Saúde Pública 38, 326–328. PMid:15122392
Radwan AS. 1975. An analytical method for proximadiol, the active principle of Cymbopogon proximus. Planta Med. 27, 93. doi:10.1055/s-0028-1097767 PMid:1161881
Rauha JP, Remes S, Heinonen M, Hopia A, Kahkonen M, Kujala T, Pihlaja K, Vuorela H, Vuorela P. 2000. Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. Int. J. Food Microbiol. 56, 3–12. doi:10.1016/S0168-1605(00)00218-X
Robert S, Anders RL, Niels F, Frank E. 2003. Evaluation of different disk diffusion/media for detection of methicillin resistance in Staphylococcus aureus and coagulasenegative staphylococci. APMIS 111, 905-914. doi:10.1034/j.1600-0463.2003.1110909.x PMid:14510648
Saikia D, Khanuja SPS, Kahol AP, Gurta AP, Kumar S. 2001. Comparative antifungal activity of essential oils and constituents from three distinct genotypes of Cymbopogon spp. Current Science 80, 1264−1266.
Sayed D. 1980. Traditional medicine in health care. Journal of Ethnopharmacology 2, 19 – 22. doi:10.1016/0378-8741(80)90023-9
Siddiqui MS, Misra LN, Nigam IM, Abu-Futuh IM. 1980. Chemotaxonomy of Cymbopogon: Gas chromatographic examination of essential oil of Cymbopogon proximus. Parfuemeric und Kosmetik 61, 419-420.
Sokmen A, Jones BM, Erturk M. 1999. The in vitro antibacterial activity of Turkish plants. Journal of Ethnopharmacology 67, 79–86. doi:10.1016/S0378-8741(98)00189-5
Taeckholm V. 1974. Students Flora of Egypt. 2nd Ed.; Cairo University Press: Cairo, 759.
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