The incorporation of polyunsaturated fatty acids into the sn-1 position of egg-yolk phosphatidylcholine (PC) in the process of lipase-catalyzed interesterification was investigated. For this purpose plant oils containing these acids in the triacylglycerol (TAG) form were used as acyl donors and three commercially available immobilized lipases were examined as biocatalysts. In all the experiments the best results were obtained using Novozym 435. After 72 h of the reaction of PC with linseed oil the maximum incorporation of α-linolenic acid into PC was 34%. The result of this reaction was also a reduction in the n-6/n-3 ratio in egg-yolk PC from 24.5 to 0.7. The highest incorporation n-6 PUFAs into PC were obtained with evening primrose oil as the acyl donor, and in this case, 50.7% of n-6 PUFA as the sum of linoleic and γ-linolenic was achieved. The highest content of γ-linolenic acid in modified PC (7.3%) was achieved in the reaction of PC with borage oil.
RESUMEN
Interesterificación de fosfatidilcolina de yema de huevo y aceites vegetales catalizada por lipasas. Se ha investigado la incorporación de ácidos grasos poliinsaturados en la posición sn-1 de fosfatidilcolina de yema de huevo (PC) en el proceso de interesterificación catalizado por lipasas. Para lograr este propósito, fueron examinados aceites vegetales que contienen estos ácidos en la forma de triacilgliceroles (TAG) como donadores de acilo y tres lipasas inmovilizadas disponibles comercialmente. En todos los experimentos los mejores resultados se obtuvieron para Novozym 435. La incorporación máxima de ácido α-linolénico en PC fue del 34% después de 72h de la reacción de PC con aceite de linaza. El resultado de esta reacción fue también la reducción de la relación de n-6/n-3 en PC de yema de huevo de 24.5 a 0.7. La incorporación más alta de n-6 PUFAs en PC se logró para el aceite de onagra como donador de acilo, en este caso se alcanzó el 50.7% de n-6 PUFA, como suma de los ácidos linoleico y γ-linolénico. El mayor contenido de ácido γ-linolénico en PC modificado (7.3%) se logró en la reacción de PC con aceite de borraja.
KEYWORDSEgg-yolk phosphatidylcholineInteresterificationLipasePlant oilPolyunsaturated fatty acidsStructured phospholipidsPALABRAS CLAVEAceite vegetalÁcidos grasos poliinsaturadosFosfatidilcolina de yema de huevoFosfolípidos estructuradosInteresterificaciónLipasa1. INTRODUCTION
Polyunsaturated fatty acids (PUFA) of the n-3 series as well as those of the n-6 series are known to be essential nutrients in the human diet. Linoleic acid (LA, 18:2, n-6) and α-linolenic acid (ALA, 18:3, n-3) as essential fatty acids (EFA) are very important for maintaining bio-functions in mammals (Nagao and Yanagita, 2008; Vessby, 2003). Because of the lack of adequate desaturases human systems do not synthesize linoleic acid and α-linolenic acid. Once obtained from the diet, ALA can be further metabolized by Δ-6 desaturation, elongation and Δ-5 desaturation to eicosapentaenoic acid (EPA, 20:5, n-3) and docosahexaenoic acid (DHA, 22:6, n-3), while LA is converted by the same enzymatic route to γ-linolenic acid (GLA, 18:2, n-6) and arachidonic acid (ARA, 20:4, n-6).
It has been reported that the consumption of n-3 PUFA, especially ALA and DHA, correlates with a reduced risk of cancer and cardiovascular diseases and prevents hypertension (Berry and Hirsch, 1986; Bigger and El-Sherif, 2001; Holub and Holub, 2004; Kris-Etherton et al., 2004). DHA is a crucial factor in the normal development of brain and retina (Uauy and Dangour, 2006). ARA and EPA are the parent compounds for the production of eicosanoids whereas skin improving effects have been ascribed mainly to LA and GLA (Muggli, 2005; van Gool et al., 2004).
Phospholipids (PL) are very interesting natural compounds for many reasons. They are the main structural components of biological membranes and they take a part in cell signaling mechanisms (Dowhan, 1997). In addition, they are found in several industrial applications, e.g. as emulsifiers in pharmaceutical and food products and components of liposomes for cosmetics and drug delivery (Gabizon et al., 1997; Schneider, 2001).
Some oilseeds (e.g. soybean) and egg-yolk are particularly rich in phospholipids. The egg-yolk lecithin comprises 78% phosphatidylcholine (PC), 18% phosphatidylethanolamine (PE) and 2% cholesterol. The major fatty acids in egg-yolk PC are: palmitic (16:0; 35%), stearic (18:0; 13.4%), oleic (18:1; 30.4%), linoleic (18:2; 18%) and arachidonic (20:4; 3.2%) (Palacios and Wang, 1995). It should be emphasized that the fatty acid composition of egg-yolk lipids is strongly influenced by modifications in the hens’ diet (Cobos et al., 1995). Studies showed that when hen feed was supplemented with some plant or fish oils the enrichment of egg-yolk lipids (especially PC) with n-3 polyunsaturated fatty acids (PUFA) was observed (Gładkowski et al., 2011; Lewis et al., 2000; Fraeye et al., 2012). Nevertheless, their total content in yolk fatty acid composition did not exceed 15%.
Phospholipids as the source of essential fatty acids may be an efficient dietary vehicle to deliver these valuable components. Considerable efforts are being made to incorporate health-promoting fatty acids from the n-3 or n-6 family into PL in the production of food or cosmetic additives. Incorporating DHA and EPA into PL was reported by several authors (Haraldsson and Thorarenson, 1999; Mutua and Akoh, 1993; Kim et al., 2010; Garcia et al., 2008). Reports on the incorporation of LA, ALA or GLA especially into PC isolated from egg-yolk are limited (Ghosh and Bhattacharyya, 1997; Hara et al., 2002).
The use of lipases and phospholipases allows for the specific removal or replacement of the acyl chains at position sn-1 or sn-2 of PL via hydrolysis and reesterification or through direct interesterification using different acyl donors (Haraldsson and Thorarenson, 1999; Mutua and Akoh, 1993; Lyberg et al., 2005; Park et al., 2000; Baeza-Jimenez et al., 2012; Kim et al., 2007).
The results of our studies on the incorporation of ALA into egg-yolk PC in the sn-1 and sn-2 position have been reported previously (Chojnacka et al., 2009). Because of the fact that natural plant oils are a rich source of PUFAs in the form of triacylglycerols (TAG), in these studies we looked into the possibilities of producing ALA-, LA- or GLA-enriched PC in the lipase-catalyzed direct interesterification of egg-yolk PC with plant oils. During this reaction the acids from the sn-1 position of PC are exchanged to the acids in the sn-1 and sn-3 positions of TAGs from oils (Scheme 1). No data about the application of edible oils as reagents in the enzymatic incorporation of PUFA into phospholipids have been reported as yet.
2. MATERIALS AND METHODS2.1. Materials
Lohmann Brown hens’ eggs were a gift from the Tronina factory. Linseed oil, sunflower oil, safflower oil, borage oil and evening primrose oil were purchased from the Oleofarm Co., Poland. Lipozyme TL IM (a silica granulated Thermomyces lanuginosus. lipase preparation, 250 U/g), was a gift from the Novozymes A/S (Bagsvaerd, Denmark). Lipase B from Candida antarctica (synonym: Novozym 435 > 5,000 U/g), immobilized in a macroporous acrylic resin and lipase from Mucor miehei (Lipozyme® > 30 U/g), immobilized in an anion-exchange resin were purchased from Sigma-Aldrich. Methanol (pure p.a.), hexane (pure p.a.), acetone (pure p.a.) and chloroform (pure p.a.) as well as NaOH, NaCl and anhydrous MgSO4 were purchased from Chempur® (Poland, Piekary Śląskie). HPLC grade solvents: 2-propanol, n-hexane (used also for the enzymatic reaction), and a boron trifluoride methanol complex solution (13–15% BF3 × MeOH) were purchased from Sigma–Aldrich. Boron trifluoride ethyl etherate (48% BF3/Et2O) was bought from Fluka. Ethanol (95%) was purchased from POCh (Poland, Gliwice). The silica gel (Kieselgel 60, 230–400 mesh) used in the column chromatography and silica gel-coated aluminium plates (Kieselgel 60 F254, 0.2 mm) used in thin layer chromatography (TLC) were purchased from Merck.
2.2. Isolation of PC from egg-yolk
A pure phospholipid fraction (1.35 g) was isolated from 20 g of the fresh egg-yolk by the extraction with ethanol and precipitation of crude PL-fraction with cold acetone (Gładkowski et al., 2012). The pure PC (0.9 g) was separated from this fraction by silica gel column chromatography (chloroform/methanol/water, 65:25:4, v/v/v).
2.3. The lipase-catalyzed interesterification of PC with plant oils
The reactions were carried out using three different lipases, in N2 atmosphere, at 55 °C. They were initiated by adding 270 mg of enzyme (20% w/w of total substrates) to the mixture containing egg-yolk PC (0.52 mmol, 400 mg) and plant oil (1.05 mmol) in 5 mL of hexane. The reaction mixture was agitated in a magnetic stirrer at 300 rpm. All trials were carried out in duplicate. The reactions were stopped at the selected time interval by enzyme filtration and modified PC and LPC were separated from the mixtures by column chromatography and then converted to fatty acid methyl esters (FAME).
2.4. Analysis
High performance liquid chromatography (HPLC) analysis was carried out using a DIONEX UltiMate 3000 LC system equipped with a CORONA CAD detector (ESA Biosciences, Chelmsford, MA). A Waters Spherisorb S5W column (150 × 4.6 mm) was used for analysis. The HPLC gradient program (flow rate 1mL·min−1) was set as follows: at 0 min 59/40/1 (%A/%B/%C), at 6 min 52/40/8, at 21 min 59/40/1, then held for 5 min (A/B/C = water/hexane/2-propanol).
The progress of enzymatic reactions and purity of the products were monitored by TLC on silica gel-coated aluminium plates (chloform/methanol/water, 65:25:4, v/v/v). The purity of the modified phosphatidylcholine was determined by HPLC after prior purification by means of column chromatography on silica gel using the same eluent as mentioned above.
2.4.1. Preparation and analysis of FAME
Samples (50 mg) were dissolved in 2 mL of a 0.5 M methanolic NaOH solution. After heating for 2 minutes under reflux 1 mL of BF3 × MeOH complex solution was added and the mixtures were heated again for 3 minutes. After cooling the mixture was extracted with 1 mL of hexane and the organic layer was washed with a saturated NaCl solution. Hexane extracts were dried over anhydrous magnesium sulphate (MgSO4) and analyzed directly by GC using a 70% cyanopropyl polysilphenylene-siloxane column (TR FAME, 30 m × 0.25 mm × 0.25 μm) and FID detector. The temperature parameters were as follows: injector 250 °C, detector 280 °C, column: 160 °C (held 3 min), 160–220 °C (rate 5 °C·min−1), 220–260 °C (rate 30 °C·min−1), 260 °C (held 3 min). Total time: 19.33 min. FAs were identified by comparing the retention times of their methyl esters with a standard FAMEs mixture (Supelco 37 FAME Mix) purchased from Sigma-Aldrich.
2.4.2. Positional analysis of fatty acids in native and modified PC
The procedure described by Kiełbowicz et al. (2012) was applied:
Position sn-1. Ethanol (95%, 2 mL) and Lipozyme® (0.4 g) were added to PC (0.05 g) and the mixture was shaken vigorously for 8 h. The enzyme was filtered off, ethanol was evaporated and the remaining material was suspended in 2 mL of water and vigorously mixed using a vortex mixer. The obtained suspension was transferred to a 15 mL vial and 3 mL of hexane were added to extract free fatty acids (FFA) and fatty acid ethyl esters (FAEE) released from the sn-1 position of PC. The two phases obtained after extraction were separated. The water fraction containing 2-acyl LPC was left for further analysis (see “position sn-2”). The solvent (from hexane extract) was evaporated and 2 mL of anhydrous ethanol and 1 mL of 48% BF3/Et2O were added. The mixture was refluxed for 3 min to complete the conversion of FFA into FAEE. After cooling the mixture was extracted with 1 mL of hexane and the organic layer was washed with a saturated NaCl solution, dried over anhydrous MgSO4 and FAEE were analyzed directly by gas chromatography to give the composition of FA in the sn-1 position of PC. The temperature parameters during the GC analysis were the same as those for the analysis of FAME. The FAs were identified by comparing the retention times of their ethyl esters with a standard FAEEs mixture previously prepared (Kiełbowicz et al., 2012).
Position sn-2. The water fraction with 2-acyl LPC was concentrated at 60 °C in vacuo and the remaining material was dissolved in 0.5 mL of hexane and transferred to a centrifuge bottle where cold acetone (−20 °C, 10 mL) was added to precipitate 2-acyl LPC. The precipitate was carefully washed 3 times with 10 mL portions of cold acetone and the solvent was removed by decantation each time. The mixture was then centrifuged (10,000g, 1 min). The supernatant was removed and 10 mg of sample of pure 2-acyl LPC were dissolved in 2 mL of a 0.5 M ethanolic NaOH solution and heated under reflux for 2 min. Afterwards, 1 mL of 48% BF3/Et2O was added to convert the fatty acid from the sn-2-position to FAEE. Further treatment of samples before GC analysis was the same as described for the analysis of FA from the sn-1 position.
3. RESULTS AND DISCUSSION
Lipase-catalyzed egg-yolk phosphatidylcholine-plant triacylglycerol interchange reaction (Scheme 1) was applied to modify PC to increase its nutritional qualities.
The material for our studies was PC isolated from Lohmann Brown hens’ eggs. It was previously subjected to FA analysis and the results (Table 1) showed that palmitic (16:0) and oleic (18:1) were the predominating ones. The first one as well as stearic acid (18:0) are located in the sn-1 position whereas the oleic acid is the major acid in the sn-2 position. The sn-2 position is also occupied by unsaturated acids from the n-6 family (LA and ARA) (Table 2). The presence of small quantities of DHA and EPA can be observed as the result of feeding the hens with feed supplemented with dietary sources of n-3 PUFAs such as flaxseed and fish oils. The studies revealed that in this way EPA, DPA and DHA can be incorporated only at the sn-2 position (Gładkowski et al., 2011; Gładkowski et al., 2014) whereas ALA is not accumulated in the PL fraction at all (Aymond and van Elswyk, 1995).
The fatty acid profile of starting materials (% according to GC)a
FA
Native PC
Linseed oil
Safflower oil
Sunflower oil
Evening primrose oil
Borage oil
C16:0
30
5.4
7.3
7.9
6.7
9.9
C16:1
1.5
–
–
–
–
–
C18:0
15
3.3
2.8
4.9
2.0
5.7
C18:1
28
16.8
19.5
16.3
5.7
19.3
C18:2
20
16.0
70.4
70.9
74.3
40.3
C18:3 (ALA)
–
58.5
–
–
–
–
C18:3 (GLA)
–
–
–
–
10.4
20.7
C20:4 (ARA)
4.5
–
–
–
0.9
4.1
C22:6 (DHA)
1
–
–
–
–
–
Tabular entries are the average of duplicate determinations from different experimental trials.
Total and positional fatty acid composition (% according to GC) of native egg-yolk PC and structured PC (SPC) obtained after 72 hours of enzymatic transesterification of egg-yolk PC with linseed oila
FA
Native egg-yolk PC
SPC (linseed oil)b
Novozym 435
Lipozyme®
Total
sn-1
sn-2
Total
sn-1
sn-2
Total
sn-1
sn-2
C16:0
30
63
1
8
8
2
11
28
1
C16:1
1.5
2
1
1
3
–
1
1
–
C18:0
15
29
–
3
3
2
9
13
–
C18:1
28
5
47
28
9
45
31
10
51
C18:2 (LA)
20
1
43
22
8
36
23
14
35
C18:3 (ALA)
–
–
–
34
68
5
18
34
1
C20:4 (ARA)
4.5
–
6
3
1
7
7
–
12
C22:6 (DHA)
1
–
2
1
–
3
–
–
–
ΣPUFA
25.5
1
51
60
77
51
48
48
48
n-6/n-3
24.5
–
–
0.7
–
–
1.7
–
–
Tabular entries are the average of duplicate determinations from different experimental trials.
The oil used in the reaction is given in parentheses.
Unsaturated fatty acids present at the sn-2 position can be released in humans only by phospholipase A2. In order to make them more bioavailable we decided to apply 1,3-regioselective lipases to the modification of egg-yolk PC. The introduction of desired fatty acids in the sn-1 position will make them more susceptible to the lipases present in our digestive track.
As the acyl donors we chose commercial plant oils in which ALA, LA and GLA are present in the form of triacylglycerols. The fatty acid composition of TAGs from these oils (Table 1) indicates that the content of linoleic acid in safflower, sunflower and evening primrose oils is 70–75% and linseed oil is the richest source of α-linolenic acid (58.5%). Additionally, evening primrose oil and borage oil contain γ-linolenic acid (10.4 and 20.7%, respectively). A stereospecific analysis in edible plant oils showed that with some exceptions such as cocoa butter or peanut oil, unsaturated fatty acids are almost equally distributed in all positions of the glycerol skeleton (Brockerhoff and Yurkowski 1966; Christie et al., 1991).
3.1. Interesterification of egg-yolk PC with linseed oil
The fortification of phospholipids with ALA is especially desirable because of the metabolism pathway in which ALA is converted to EPA and DHA by the elongation and desaturation of the hydrocarbon chain. Previous studies on the preparation of ALA-enriched PC were limited to the acidolysis process of egg-yolk PC with ALA (Chojnacka et al., 2009), the transesterification of soy lecithin with the methyl esters of linseed oil (Ghosh and Bhattacharyya, 1997) and transacylation of phospholipids with synthetic monolinolenin (Hara et al., 2002). Due to economic reasons it is desirable to look for inexpensive acyl donors for that purpose. Therefore we decided to use linseed oil, a natural source of ALA, as an acyl donor in lipase-catalyzed direct interesterification with PC. The fatty acid composition of linseed oil TAG (Table 1) revealed that it contains almost 60% of ALA whereas the purity of commercially available ALA is about 70%.
Three commercially available lipases: Lipozyme®, Lipozyme TL IM and immobilized Candida antarctica lipase B (Novozym 435) were tested in our studies. Two first enzymes are classified as 1,3-regiospecific lipases (Guo et al., 2005), the third one is classified as non regiospecific lipase but shows high selectivity towards the sn-1 position (Virto and Adlecreutz, 2000).
The specificity of these enzymes prevents the transesterification of unsaturated fatty acids at the sn-2 position of PC. As a result, only the saturated fatty acids from the sn-1 position of PC are exchanged during the interesterification process to the unsaturated fatty acids from the sn-1 and sn-3 positions of TAGs from plant oils.
Novozym 435 is possibly the most common lipase used as biocatalyst in organic synthesis (Anderson et al., 1998). This immobilized preparation is obtained by the immobilization of lipase B from Candida antarctica via the interfacial activation of the enzyme on a moderately hydrophobic resin, Lewatit VP OC 1600 (Uppenberg et al., 1994).
Lipozyme TL IM also has been used in multiple reactions (Fernandez-Lafuente, 2010). It is an immobilized lipase from Thermomyces lanuginosus produced by a genetically modified strain of Aspergillus oryzae and immobilized on a silicate via ionic adsorption (Peng et al., 2002).
Immobilized lipase from Rhizomucor miehei (RML) has been produced and used mainly in the modification of oils and fats, but it has also been also successfully applied in many reactions in organic chemistry (Rodrigues and Fernandez-Lafuente, 2010). The support of this immobilized enzyme is Duolite ES 562, a weak anion exchange resin based on phenol-formaldehyde copolymers (Huge-Jensen et al., 1987).
All of these lipases are commonly used for the modification of natural acylglycerols (Gunstone, 1999; Lee and Akoh, 1998; Fomuso and Akoh, 1998; Akoh et al., 1995) and synthetic or natural phospholipids (Vikbjerg et al., 2005; Lyberg et al., 2005; Peng et al., 2002; Mutua and Akoh, 1993). They also showed activity in the acidolysis of PC with ALA (Chojnacka et al., 2009), therefore they were chosen as biocatalysts in the present studies.
The conditions applied for the reaction systems in our present investigations were also chosen on the basis of earlier studies (Chojnacka et al., 2009). They were as follows: 20% lipase dosage (based on the weight of substrates), temperature 55 °C and hexane as a solvent. The only difference was a lower linseed oil/PC molar ratio (2:1), because the acyl donors (TAGs in linseed oil) contain two acyl chains (at sn-1 and sn-3 positions) susceptible to enzyme action.
The time course of the incorporation of ALA into PC by lipases is shown in Fig. 1. Among the lipases Novozym 435 exhibited the highest activity giving 34% incorporation of ALA in 72 h, whereas Lipozyme® afforded 18% of ALA in modified PC. Lipozyme TL IM showed the lowest activity in this process and only 4% of ALA was detected in the modified PC. When the reaction was continued for more than 72 h no significant increase in ALA in modified PC was observed and the total yield of PC was reduced due to the formation of lyso-PC. Adlecreutz and co-workers have reported that the loss of PC in the reaction system may occur via hydrolysis, a side-reaction leading to the formation of 2-acyl LPC in the presence of lipase (Adlecreutz et al., 2002). Moreover, hydrolysis also produces glycerophosphatidylcholine (GPC) from 1-acyl LPC (obtained from 2-acyl LPC via acyl migration). In our studies the isolated yield of the structured PC ranged from 35 to 55%.
Increase (%) in the α-linolenic acid (ALA) content in egg-yolk PC during transesterification with linseed oil catalyzed by three different lipases.
Similar differences in activity of the enzymes used (Novozym 435 > Lipozyme® > Lipozyme TL IM) were observed by us earlier during the acidolysis of egg-yolk PC with ALA (Chojnacka et al., 2009). The incorporation of ALA was slightly lower (28% in the case of Novozym 435) than that obtained in the interesterification process described here. On the other hand, Ghosh et al. (1997) modified soy lecithin using the methyl esters (ME) of linseed oil (53.5% purity) in interesterification catalyzed by immobilized lipase from M. miehei with a 1:5 molar ratio of substrates (PC:ME). They achieved only 10.2% of ALA incorporation after 48 hours of reaction increasing its content in PC from 5.2% to 15.4%. Hara et al. (2002) carried out the transacylation of soy PC with monolinolenin (18:3-MG) and no regiospecific lipase OF originating in Candida rugosa and they reported an increase in ALA in soy PC from 6.3 to 19.9%, after 48 hours of reaction at 37 °C.
Analyzing the total and positional FA composition of the native and modified egg-yolk PC (Table 2) one can see that the increase of ALA was accompanied by a reduction in palmitic (16:0) and stearic acid (18:0). In the case of palmitic acid an approximately 3-fold decrease in its content was observed whereas the amount of stearic acid was reduced by even 5-fold. On the other hand, small increases in oleic and linoleic acid (18:2) were noticed as a consequence of the FA composition of linseed oil which contained almost equal (16%) amounts of both acids.
The results of the positional analysis show that ALA was introduced mainly in the sn-1 position of PC which is in accordance with the 1,3-regiospecificity of the lipases studied.
The result of the reaction of PC with linseed oil was not only the incorporation of ALA into PC but also a reduction in the n-6/n-3 ratio in egg-yolk PC from 24.5 to 0.7 in the case of Novozym 435 and to 1.7 in the case of Lipozyme® (Table 2). This feature is very positive in the context of the production of dietary enriched egg-yolk PC as a food supplement because an increase in the dietary intake of n-6 PUFA changes the physiological state to a prothrombotic and proinflammatory state (Simopoulos, 2002).
3.2. Interesterification of egg-yolk PC with plant oils riched in <italic>n</italic>-6 PUFAs
Four different plant oils were used as n-6-PUFAs acyl donors in the transesterification of egg-yolk phosphatidylcholine in order to obtain LA-enriched PC and GLA-enriched PC which are useful in cosmetic formulations. The fatty acid compositions of these plant oils are shown in Table 1. Safflower oil and sunflower oil predominantly contain linoleic acid (over 70%). Evening primrose oil is the richest source of n-6 PUFA, due to the fact that besides the highest amount of LA it also contains a considerable amount of γ-linolenic acid (GLA, 10.4%). Borage oil is known as the richest natural source of GLA (20.4%).
For the incorporation of n-6 PUFAs at the sn-1 position of egg yolk PC we tried the same reaction system and biocatalysts as for the reaction of PC with linseed oil. All the reactions were carried out for 72 h.
In the transesterification of egg-yolk PC with TAGs rich in linoleic acid (safflower, sunflower and evening primrose oils), comparable results were obtained (Tables 3 and 4). The maximum incorporation of LA into PC was 46.7% from an initial content of 20%, using Novozym 435 and evening primrose oil. With safflower and sunflower oils, at the presence of this enzyme linoleic acid was incorporated into egg-yolk PC up to 43.9 and 38.7%, respectively. The percentage incorporation of LA was higher than obtained by Hara et al. (2002) in the transacylation of egg-yolk PC with monolinolein (18:2-MG) in the presence of lipase OF. They reported an increase in the linoleic acid content in modified PC from 7.6 to 28%.
Fatty acid composition (% according to GC) of egg-yolk PC and structured PC (SPC) obtained after 72 h of enzymatic transesterification of egg-yolk PC with safflower and sunflower oila
FA
native PC
SPC (safflower oil)b
SPC (sunflower oil)
Novozym 435
Lipozyme®
Lipozyme TL IM
Novozym 435
Lipozyme®
Lipozyme TL IM
C16:0
30
8.8
21.7
19.8
13.9
19.9
24.5
C16:1
1.5
–
–
–
–
–
C18:0
15
5.4
11.9
13.2
8.0
12.7
14.1
C18:1
28
39.5
32.9
33.0
35.7
31.3
30.9
C18:2
20
43.9
28.7
28.8
38.7
27.5
26.5
C20:4
4.5
2.4
4.8
5.2
3.7
4.1
4.0
Σn-6PUFA
24.5
46.3
33.5
34
42.4
31.6
30.5
Tabular entries are the average of duplicate determinations from different experimental trials.
The oil used in the reaction is given in parentheses.
Fatty acid composition (% according to GC) of egg-yolk PC and structured PC (SPC) obtained after 72 h of enzymatic transesterification of egg-yolk PC with borage and evening primrose oila
FA
native PC
SPC (borage oil)b
SPC (evening primrose oil)
Novozym 435
Lipozyme®
Lipozyme TL IM
Novozym 435
Lipozyme®
Lipozyme TL IM
C16:0
30
15.7
21.9
23.4
9.4
20.0
18.9
C16:1
1.5
–
–
–
–
–
C18:0
15
9.2
12.7
13.8
4.5
10.7
9.6
C18:1
28
35.5
33.8
32.7
32.2
30.4
33.7
C18:2
20
28.2
25.6
24.3
46.7
33.3
34.3
C18:3 (GLA)
–
7.3
0.9
0.6
4.0
0.6
0.8
C20:4
4.5
4.1
5.1
5.2
3.2
5.0
4.0
Σn-6PUFA
24.5
39.6
31.6
30.1
53.9
38.9
39.1
Tabular entries are the average of duplicate determinations from different experimental trials.
The oil used in the reaction is given in parentheses.
Transesterification between egg-yolk PC and LA-rich oils with Lipozyme TL IM or Lipozyme® as biocatalysts allowed for the production of structured PC with similar linoleic acid contents ranging from 26.5 to 34.3%. Contrary to our previous experimental trials with linseed oil as an acyl donor it was found that Lipozyme TL IM had a slightly better ability to incorporate linoleic acid into PC than α-linolenic acid. This phenomenon can be explained by the slight discrimination of very long chain PUFAs by different lipases. Peng et al. (2002) reported that Lipozyme TL IM had a better possibility to incorporate caprylic acid (C8:0) into PLs than EPA and DHA.
Our trials to enrich egg-yolk PC in γ-linolenic acid were not so effective because of the fact that the amount of GLA in borage and evening primrose oils is significantly lower than the amount of ALA and LA in oils used in previous experiments. However, using Novozym 435 turned out to be the most successful and allowed for the incorporation of 7.3% and 4% of GLA into PC when borage and evening primrose oils were used as acyl donors, respectively. Lipozyme TL IM and Lipozyme® revealed very low activity in the interesterification of egg-yolk PC and borage oil.
The results of positional analysis of n-6 enriched PC (Table 5) showed the incorporation of LA and GLA in the sn-1 position, similar to the incorporation of ALA (Table 2).
Positional distribution of LA and GLA (% according to GC) in native egg-yolk PC and n-6-enriched phosphatidylcholine (n-6-PC) obtained after 72 hours of Novozym 435-catalyzed transesterification of egg-yolk PC with different oilsa
FA
Native egg-yolk PC
n-6-PC(safflower oil)b
n-6-PC(sunflower oil)
n-6-PC(borage oil)
n-6-PC(evening primrose oil)
Total
sn-1
sn-2
Total
sn-1
sn-2
Total
sn-1
sn-2
Total
sn-1
sn-2
Total
sn-1
sn-2
C18:2(LA)
20
1
43
43.9
40.3
42.1
38.7
36
43.2
28.2
25.4
43.5
46.7
46.7
41
C18:3 (GLA)
–
–
–
–
–
–
–
–
–
7.3
12
1
4.0
6.6
0.8
Tabular entries are the average of duplicate determinations from different experimental trials.
The oil used in the reaction is given in parentheses.
4. CONCLUSIONS
Phosphatidylcholine from egg-yolk was modified successfully by the introduction of the residues of polyunsaturated fatty acids from the n-3 and n-6 family derived from plant oils. In our studies the modification of PC by immobilized lipase from Candida antarctica (Novozym 435) and linseed oil (source of ALA, n-3 PUFA) or evening primrose oil (source of LA and GLA, both n-6 PUFAs) gave products with very attractive fatty acid profiles (Fig. 2). The SPC obtained from the transesterification of egg-yolk PC with linseed oil contained 34% of n-3 PUFAs, whereas that obtained with evening primrose oil had over 53% of n-6 PUFAs. In the case of both structured PC the contents of saturated acid did not exceed 14%. Additionally, the modification of PC with linseed oil allowed for the reduction of the n-6/n-3 ratio in egg-yolk PC from 24.5 to 0.7 or 1.7, using Novozym 435 and Lipozyme®, respectively.
Fatty acid profiles of native egg-yolk PC and structured PC (SPC) obtained in the transesterifications with different plant oils after 72 h of reaction catalyzed by Novozym 435 in hexane.
The cost of the process is lowered due to replacement of synthetic acyl donors with the cheaper natural plant oils. The optimization of reaction conditions (reaction temperature, enzyme load, water activity, PC/oil molar ratio) is still needed and will be published soon. In addition, the enzyme activity may differ depending on the immobilization form (Rodriguez, 2010) and this aspect may explain the differences in the enzymatic activities observed in our studies.
PUFA-enriched PC is recognized as a multi-functional lipid with two functionalities: PUFAs and PC. It can find applications in the nutraceutical (ALA-PC) or cosmetic (LA-PC and GLA-PC) fields.
AKNOWLEDGMENTS
This project was financed by National Science Center Project no. 2012/05/B/NZ9/03358.
REFERENCESAdlercreutzD.BuddeH.WehtjeE.Synthesis of phosphatidylcholine with defined fatty acid in the sn-1 position by lipase-catalyzed esterification and transesterification reactionBiotechnol. Bioeng.200278403411http://dx.doi.org/10.1002/bit.10225.AkohCC.JenningsBH.LillardDA.Enzymatic modification of trilinolein: Incorporation of n-3 polyunsaturated fatty acidsJ. Am. Oil Chem. Soc.19957213171321http://dx.doi.org/10.1007/BF02546205.AndersonEM.LarssonKM.KirkO.One Biocatalyst–Many Applications: The Use of Candida Antarctica B-Lipase in Organic SynthesisBiocatal. Biotransform.199816181204http://dx.doi.org/10.3109/10242429809003198.AymondWM.Van ElswykME.Yolk thiobarbituric acid reactive substances and n-3 fatty acids in response to whole and ground flaxseedPoult. Sci.19957413881394http://dx.doi.org/10.3382/ps.0741388.Baeza-JiménezR.González-RodríguezJ.KimI-H.GarcíaHS.OteroC.Use of immobilized phospholipase A1-catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid contentGrasas Aceites2012634452http://dx.doi.org/10.3989/gya.045211.BerryEJ.HirschJ.Does dietary linolenic acid influence blood pressure?Am. J. Clin. Nutr.198644336340BiggerJT.El-SherifT.Polyunsaturated fatty acids and cardiovascular events. A fish taleCirculation.2001103623625http://dx.doi.org/10.1161/01.CIR.103.5.623.BrockerhoffH.YurkowskiM.Stereospecific analyses of several vegetable fatsJ Lipid Res.196676264ChojnackaA.GładkowskiW.KiełbowiczG.WawrzeńczykC.Enzymatic enrichment of egg-yolk phosphatidylcholine with α-linolenic acidBiotechnol. Lett.200931705709http://dx.doi.org/10.1007/s10529-009-9915-6.ChristieWW.Nikolova-DamyanovaB.LaaksoP.HerslofB.Stereospecific analysis of triacyl-sn-glycerols via resolution of diastereomeric diacylglycerol derivatives by HPLC on silicaJ. Am. Oil Chem. Soc.199168695701http://dx.doi.org/10.1007/BF02662155.CobosA.de la HozL.CamberoMI.OrdóñezJA.Dietary modification and hen strain dependence of egg yolk lipidsFood Res. Int.1995287176http://dx.doi.org/10.1016/0963-9969(95)93333-P.DowhanW.Molecular basis for membrane phospholipid diversity: why are there so many phosholipids?Annu. Rev. Biochem.199766199232http://dx.doi.org/10.1146/annurev.biochem.66.1.199.Fernandez-LafuenteR.Lipase from Thermomyces lanuginosus: Uses and prospects as an industrial biocatalystJ. Mol. Catal. B: Enzym.201062197212http://dx.doi.org/10.1016/j.molcatb.2009.11.010.FomusoLB.AkohCC.Structured lipids: Lipase-catalyzed interesterification of tricaproin and trilinoleinJ. Am. Oil Chem. Soc.199875405410http://dx.doi.org/10.1007/s11746-998-0059-y.FraeyeI.BruneelC.LemahieuC.BuyseJ.MuylaertK.FoubertI.Dietary enrichment of eggs with omega-3 fatty acids: A reviewFood Res. Int.201248961969http://dx.doi.org/10.1016/j.foodres.2012.03.014.GabizonA.GorenD.HorowitzAT.TzemachD.LossosA.SiegalT.Long-circulating liposomes for drug delivery in cancer therapy: a review of biodistribution in tumor-bearing animalsAdv. Drug Deliv. Res.199724337344http://dx.doi.org/10.1016/S0169-409X(96)00476-0.GarciaHS.KimI-H.Lopez-HernandezA.HillCG.Jr.Enrichment of lecithin with n-3 fatty acids by acidolysis using immobilized phospholipase A1Grasas Aceites200859368374http://dx.doi.org/10.3989/gya.2008.v59.i4.531.GhoshM.BhattacharyyaDK.Soy lecithin – monoester interchange reaction by microbial lipaseJ. Am. Oil Chem. Soc.199774761763http://dx.doi.org/10.1007/s11746-997-0215-9.GładkowskiW.KiełbowiczG.ChojnackaA.BobakŁ.SpychajR.DobrzańskiZ.TrziszkaT.WawrzeńczykC.The effect of feed supplementation with dietary sources of n-3 polyunsaturated fatty acids, flaxseed and algae Schizochytrium sp., on their incorporation into lipid fractions of Japanese quail eggsInt. J. Food Sci. Tech.20144918761885http://dx.doi.org/10.1111/ijfs.12497.GładkowskiW.ChojnackaA.KiełbowiczG.TrziszkaT.WawrzeńczykC.Isolation of pure phospholipid fraction from egg-yolkJ. Am. Oil Chem. Soc.201289179182http://dx.doi.org/10.1007/s11746-011-1893-x.GładkowskiW.KiełbowiczG.ChojnackaA.GilM.TrziszkaT.DobrzańskiZ.WawrzeńczykC.Fatty acid composition of egg yolk phospholipid fractions as the effect of feed supplementation of Lohmann Brown hens with humine-fat preparationsFood Chem.201112610131018http://dx.doi.org/10.1016/j.foodchem.2010.11.112.Van GoolCJ.ZeegersMP.ThijsC.Epidemiology and health services research. Oral essential fatty acids supplementation in atopic dermatitis – a meta-analysis of placebo-controlled trialsBr. J. Dermatol.2004150728740http://dx.doi.org/10.1111/j.0007-0963.2004.05851.x.GunstoneFD.Enzymes as biocatalysts in the modification of natural lipidsJ. Sci. Food Agric.19997915351549http://dx.doi.org/10.1002/(SICI)1097-0010(199909)79:12<1535::AID-JSFA430>3.0.CO;2-7.GuoZ.VikbjergAF.XuX.Enzymatic modification of phospholipids for functional applications and human nutritionBiotechnol. Adv.200523203259http://dx.doi.org/10.1016/j.biotechadv.2005.02.001.HaraS.HasuoH.NakasatM.HigakiY.TotaniY.Modification of soybean phospholipids by enzymatic transacylationJ. Oleo Sci.200251417422http://dx.doi.org/10.5650/jos.51.417.HaraldssonGG.ThorarensonA.Preparation of phospholipids highly enriched with n-3 polyunsaturated fatty acids by lipaseJ. Am. Oil. Chem. Soc.19997611431149http://dx.doi.org/10.1007/s11746-999-0087-2.HolubDJ.HolubBJ.Omega-3 fatty acids from fish oils and cardiovascular diseaseMol. Cell. Biochem.2004263217225http://dx.doi.org/10.1023/B:MCBI.0000041863.11248.8d.Huge-JensenB.GalluzzoDR.JensenRG.Partial purification and characterization of free and immobilized lipases from Mucor mieheiLipids198722559565http://dx.doi.org/10.1007/BF02537281.KiełbowiczG.GładkowskiW.ChojnackaA.WawrzeńczykC.A simple method for positional analysis of phosphatidylcholineFood Chem.201213525422548http://dx.doi.org/10.1016/j.foodchem.2012.07.005.KimI-H.GarciaHS.HillCG.Jr.Synthesis of Structured Phosphatidylcholine Containing n-3 PUFA Residues via Acidolysis Mediated by Immobilized Phospholipase A1J. Am. Oil. Chem. Soc.20108712931299http://dx.doi.org/10.1007/s11746-010-1609-7.KimI-H.GarciaHS.HillCG.Jr.Phospholipase A1-catalyzed synthesis of phospholipids enriched in n−3 polyunsaturated fatty acid residuesEnzyme Microb. Tech.20074011301135http://dx.doi.org/10.1016/j.enzmictec.2006.08.018.Kris-EthertonPM.HeckerKD.BinkoskiAE.Polyunsaturated fatty acids and cardiovascular healthNutr. Rev.200462414426http://dx.doi.org/10.1111/j.1753-4887.2004.tb00013.x.LeeK-T.AkohCC.Solvent-free enzymatic synthesis of structured lipids from peanut oil and caprylic acid in a stirred tank batch reactorJ. Am. Oil. Chem. Soc.19987515331537http://dx.doi.org/10.1007/s11746-998-0090-z.LewisNM.SeburgS.FlanaganNL.Enriched eggs as a source of n-3 polyunsaturated fatty acids for humansPoultr. Sc.200079971974http://dx.doi.org/10.1093/ps/79.7.971.LybergA-M.AdlercreutzD.AdlercreutzP.Enzymatic and chemical synthesis of phosphatidylcholine regioisomers containing eicosapentaenoic acid or docosahexaenoic acidEur. J Lipid Sci. Tech.2005107279290MuggliR.Systemic evening primrose oil improves the biophysical skin parameters of healthy adultsInt. J. Cosmetic Sci.200527243249http://dx.doi.org/10.1111/j.1467-2494.2005.00274.x.MutuaLN.AkohCC.Lipase–catalyzed modification of phospholipids: incorporation of n-3 fatty acids into biosurfactantsJ. Am. Oil Chem. Soc.199370125128http://dx.doi.org/10.1007/BF02542613.NagaoK.YanagitaT.Bioactive lipids in metabolic syndromeProg. Lipid Res.200847127146http://dx.doi.org/10.1016/j.plipres.2007.12.002.PalaciosLE.WangT.Egg-yolk lipid fractionation and lecithin characterizationJ. Am. Oil Chem. Soc.200582571578http://dx.doi.org/10.1007/s11746-005-1110-5.ParkCW.KwonSJ.HanJJ.RheeJS.Transesterification of phosphatidylcholine with eicosapentaenoic acid ethyl ester using phospholipase A2 in organic solventBiotechnol. Lett.200022147150http://dx.doi.org/10.1023/A:1005620707624.PengL.XuX.MuH.HøyC-E.Adler-NissenJ.Production of structured phospholipids by lipase-catalyzed acidolysis: optimization using response surface methodologyEnzyme Microb. Technol.200231523532http://dx.doi.org/10.1016/S0141-0229(02)00147-3.RodriguesRC.Fernandez-LafuenteR.Lipase from Rhizomucor miehei as an industrial biocatalyst in chemical processJ. Mol. Catal. B: Enzym201064122http://dx.doi.org/10.1016/j.molcatb.2010.02.003.SimopoulosAP.The importance of the ratio of omega-6/omega-3 essential fatty acidsBiomed. Pharmacother.200256365379http://dx.doi.org/10.1016/S0753-3322(02)00253-6.SchneiderM.Phospholipids for functional foodEur. J. Lipid Sci. Tech.200110498101http://dx.doi.org/10.1002/1438-9312(200102)103:2<98::AID-EJLT98>3.3.CO;2-7.UauyRMD.DangourAD.Nutrition in Brain Development and Aging: Role of Essential Fatty AcidsNutr. Rev.2006642433http://dx.doi.org/10.1111/j.1753-4887.2006.tb00242.x.UppenbergJ.PatkarS.BergforsT.JonesTA.Crystallization and Preliminary X-ray Studies of Lipase B from Candida antarcticaJ. Mol. Biol.1994235790792http://dx.doi.org/10.1006/jmbi.1994.1035.VessbyB.Dietary fat, fatty acid composition in plasma and the metabolic syndromeCurr. Opin. Lipidol.2003141519http://dx.doi.org/10.1097/00041433-200302000-00004.VikbjergAF.MuH.XuX.Parameters affecting incorporation and by-product formation during the production of structured phospholipids by lipase-catalyzed acidolysis in solvent free systemJ. Mol. Catal. B2005361421http://dx.doi.org/10.1016/j.molcatb.2005.07.002.VirtoC.AdlecreutzP.Lysophosphatidylcholine synthesis with Candida antarctica lipase B (Novozymes 435)Enzyme Microb. Tech.200026630635http://dx.doi.org/10.1016/S0141-0229(00)00147-2.