Relevance of nitrate and nitrite in dry-cured ham and their effects on aroma development


  • Fidel Toldrá Instituto de Agroquímica y Tecnología de Alimentos (CSIC)
  • M-Concepción Aristoy Instituto de Agroquímica y Tecnología de Alimentos (CSIC)
  • Mónica Flores Instituto de Agroquímica y Tecnología de Alimentos (CSIC)



Aroma, Dry-cured ham, Flavour, Nitrate, Nitrite, Nitrosamines


Potassium and sodium salts of nitrite (E 249 and E 250) and nitrate (E 251 and E 252) are authorised for use under certain levels in several foodstuffs such as non-heat-treated, cured and dried meat products, other cured meat products, canned meat products and bacon. The key point in the use of nitrate and nitrite as preservatives is to find a balance between ensuring the microbiological safety of the ham and keeping as low as possible the level of nitrosamines in the final product. Nitrites and nitrates are authorised as additives for dry-cured ham in the Directive 2006/52/EC of 5 July 2006 that modifies previous Council Directive 95/2/EC on food additives other than colours and sweeteners. The effect of nitrate and its reduction to nitrite in controlling the lipid oxidation process during the ham ripening is very important for the development of the characteristic cured flavour. The main benefits and drawbacks of the use of nitrites and nitrates in dry-cured ham and how these levels may affect its flavour are discussed in this manuscript.


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Arnau J, Guerrero L, Casademont G, Gou P. 1995. Physical and chemical changes in different zones of normal and PSE dry cured ham during processing. Food Chem.52, 63-69. doi:10.1016/0308-8146(94)P4182-F

Baines DA; Mlotkiewicz JA. 1984. The chemistry of meat flavor, in Bailey AJ (Ed.); Recent Advances in the Chemistry of Meat, 119-164, The Royal Society of Chemistry, Burlington House, London.

Barbieri G; Bolzoni L; Parolari G; Virgili R; Buttini R; Careri M; Mangia A. 1992. Flavor compounds of dry-cured ham. J. Agric. and Food Chem. 40, 2389-2394. doi:10.1021/jf00024a013

Berdagué JL, Denoyer C, LeQueré JL, Semon E. 1991. Volatile Components of Dry-Cured Ham. J. Sci. Food Agric. 39, 1257-1261. doi:10.1021/jf00007a012

Bolzoni L, Barbieri G, Virgili R. 1996. Changes in volatile compounds of parma ham during maturation. Meat Science, 43, 301-310. doi:10.1016/S0309-1740(96)00002-2

Buscailhon S, Berdagué JL, Monin G. 1993. Time related changes in volatile compounds of lean tissue during processing of French Dry-Cured Ham. J. Sci. Food Agric. 63, 69-75. doi:10.1002/jsfa.2740630112

Careri M, Mangia A, Barbieri G, Bolzoni L, Virgili R, Parolari G. 1993.Sensory property relationships to chemical data of Italian type dry-cured ham. J. Food Sci. 58, 968-972. doi:10.1111/j.1365-2621.1993.tb06090.x

Carrapiso AI, Jurado A, Timon ML, García C. 2002a. Odor-active compounds of Iberian hams with different aroma characteristics. J. Agric. Food Chem. 50, 6453- 6458. doi:10.1021/jf025526c

Carrapiso AI, Ventanas J, García C. 2002b. Characterization of the most odor-active compounds of Iberian ham headspace. J. Agric. Food Chem. 50, 1996-2000. doi:10.1021/jf011094e

Cassens RG, Greaser ML, Ito T, Lee M. 1979. Reactions of nitrite in meat. Food Technol. 33, 46-57.

Cassens RG. 1997. Composition and safety of cured meats in the USA. Food Chem. 59, 561-566. doi:10.1016/S0308-8146(97)00007-1

Chang SS, Petersen RJ. 1977. Recent developments in the flavor of meat. J. Food Sci. 42, 298-305. doi:10.1111/j.1365-2621.1977.tb01486.x

Christiansen LN. 1980. Factors influencing botulinal inhibition by nitrite. Food Technol. 34, 237.

Demeyer DI, Raemakers M, Rizzo A, Holck A, De Smedt A, Ten Brink B, Hagen B, Montel C, Zanardi E, Murbrek E, Leroy F, Vanderdriessche F, Lorentsen K, Venema K, Sunesen L, Stahnke L, De Vuyst L, Talon R, Chizzolini R, Eerola S. 2000. Control of bioflavor and safety in fermented sausages: first results of a European project. Food Res. Int. 33, 171-180. doi:10.1016/S0963-9969(00)00031-4

Dirinck P, Van Opstaele F, Vandendriessche F. 1997. Flavour differences between northern and southern European cured hams. Food Chem. 59, 511-521. doi:10.1016/S0308-8146(97)00012-5

Drumm TD, Spanier AM. 1991. Changes in the content of lipid autoxidation and sulfur-containing compounds in cooked beef during storage. J. Agric. Food Chem. 39, 336-343 doi:10.1021/jf00002a023

EFSA. 2003. Opinion of the Scientific Panel on biological hazards on the request from the Commission related to the Effect of Nitrites/Nitrates on the Microbiological Safety of Meat Products. The EFSA Journal 14, 1-31.

Flores M, Grimm CC, Toldrá F, Spanier AM. 1997. Correlations of sensory and volatile compounds of Spanish “Serrano’’ dry-cured ham as a function of two processing times. J. Agric. Food Chem. 45, 2178-2186. doi:10.1021/jf960862c

Flores M., Spanier AM, Toldrá F. 1998. Flavour analysis of dry-cured ham, in Shahidi F (Ed.), Flavor of meat, meat products and seafood. 2nd edición, 320-339, Blackie Academic & Professional, Chapman & Hall, London.

Flores M, Gianelli MP, Pérez-Juan M, Toldrá F. 2007. Headspace concentration of selected dry-cured aroma compounds in model systems as affected by curing agents. Food Chem. 102, 488-493. doi:10.1016/j.foodchem.2006.04.011

Forss DA. 1972. Odor and flavor compounds from lipids. Progr. Chem. Fats other Lipids. 13, 181-258.

Frankel EN. 1984. Recent advances in the chemistry of rancidity of fats, in Bailey AJ (Ed.); Recent Advances in the Chemistry of Meat, 87-118, The Royal Society of Chemistry, Burlington House, London.

García C; Berdagué JL, Antequera T; López-bote C; Córdoba JJ; Ventanas J. 1991.Volatile components of dry-cured Iberian ham. Food Chem.41, 23-32. doi:10.1016/0308-8146(91)90128-B

Gianelli MP, Flores M, Toldrá F. 2002. Analysis of volatile compounds of dry-cured ham using different solidphase microextraction (SPME) fiber coatings. J. Sci. Food Agric.82, 1703-1709.

Gianelli MP, Flores M, Toldrá F. 2005. Interactions of soluble peptides and proteins from skeletal muscle with volatile compounds as affected by curing agents. J. Agric. Food Chem.53, 1670-1677. doi:10.1021/jf040357c

Gou P, Comaposada J, Arnau J. 2004. Moisture diffusivity in the lean tissue of dry-cured ham at different process times. Meat Science 67, 203-209. doi:10.1016/j.meatsci.2003.10.007

Hinrichsen LL; Pedersen SB. 1995. Relationship among flavor, volatile compounds, chemical changes, and microflora in Italian-type dry-cured ham during processing. J. Agric. Food Chem. 43, 2932-2940. doi:10.1021/jf00059a030

Lillard DA, Ayres JC. 1969. Flavor compounds in countrycured hams. Food Technol. 23, 117-120.

Lopez MO; De la Hoz L; Cambero MI; Gallardo E; Reglero G; Ordoñez JA. 1992.Volatile compounds of dry hams from Iberian pigs. Meat Sci. 31, 267-277. doi:10.1016/0309-1740(92)90057-B

Lorenzo JM, Fontan MCG, Franco I, Carballo J. 2008a. Biochemical characteristics of dry-cured lacon (a Spanish traditional meat product) throughout the manufacture, and sensorial properties of the final product. Effect of some additives. Food Control 19, 1148-1158. doi:10.1016/j.foodcont.2007.12.005

Lorenzo JM, Fontan MCG, Franco I, Carballo J. 2008b. Proteolytic and lipolytic modifications during the manufacture of dry-cured lacon, a Spanish traditional meat product: Effect of some additives. Food Chem. 110, 137-149. doi:10.1016/j.foodchem.2008.02.002

Lücke FK, Roberts TA. 1993. Control in meat and meat products, in: Hauschild AHW, Dodds KL (Eds), Clostridium botulinum ecology and control in foods. 177-207,Marcel Dekker Inc., New York, USA.

Muriel E, Ruiz J, Martin D, Petron MJ, Antequera T. 2004. Physico-chemical and sensory characteristics of drycured loin from different Iberian pig lines. Food Sci.Technol. Int. 10, 117-123. doi:10.1177/1082013204043766

Ockerman HW, Blumer TN, Craig HB. 1964. Volatile chemical compounds in dry-cured hams. J. Food Sci. 29, 123-129. doi:10.1111/j.1365-2621.1964.tb01706.x

Parolari G. 1996. Review: Achivements, needs and perspectives in dry-cured ham technology: the example of Parma ham. Food Sci. Technol. Int. 2, 69-78. doi:10.1177/108201329600200203

Pegg RB, Shahidi F. 2000. Nitrite curing of meat. Wiley- Blackwell, Ames, Iowa, USA.

Pérez-Juan M, Flores M, Toldrá F. 2006. Generation of volatile flavour compounds as affected by the chemical composition of different dry-cured ham sections. Eur. Food Res Technol. 222, 658-666. doi:10.1007/s00217-005-0196-6

Ramarathnam N, Rubin LJ, Diosady LL. 1991. Studies on meat flavor. 1. Qualitative and quantitative differences in uncured and cured pork. J. Agric. Food Chem. 39, 344-350. doi:10.1021/jf00002a024

Ramarathnam N, Rubin LJ, Diosady LL. 1993. Studies on meat flavor. 3. A novel method for trapping volatile components from uncured and cured pork. J. Agric. Food Chem. 41, 933-938. doi:10.1021/jf00030a019

Ruiz J, Cava R, Ventanas J, Jensen MT. 1998. Headspace solid phase microextraction for the analysis of volatiles in a meat product: Dry-cured Iberian ham. J. Agric. Food Chem. 46, 4688-4694. doi:10.1021/jf980139h

Ruiz J, Ventanas J, Cava R, Andrés A, García C. 1999. Volatile compounds of dry-cured Iberian ham as affected by the length of the curing process. Meat Sci., 52, 19-27. doi:10.1016/S0309-1740(98)00144-2

Sabio E, Vidal-Aragón MC, Bernalte MJ, Gata JL. 1998. Volatile compounds present in six types of dry-cured ham from south European countries. Food Chem. 61, 493-503. doi:10.1016/S0308-8146(97)00079-4

Seuvre AM, Espinosa-Diaz MA, Voilley A. 2002. Transfer of aroma compounds through the lipidic-aqueous interface in a complex system. J. Agric. Food Chem. 50, 1106-1110. doi:10.1021/jf010939j

Shahidi F; Rubin LJ; D’Souza, LA. 1986. Meat flavour volatiles: A review of the composition, techniques of analysis, and sensory evaluations.Crit. Reviews Food Sci. and Nutri. 24, 141-243.

Sindelar JJ, Cordray JC, Sebranek JG, Love JA. 2007. Investigating quality atytributes and consumer acceptance of uncured, no nitrate/nitrite added commercial hams, bacons and frankfurters. J. Food Sci. 72, s511-s559. doi:10.1111/j.1750-3841.2007.00486.x

Timón ML, Ventanas J, Martín L, Tejeda JF, García C. 1998. Volatile Compounds in Supercritical Carbon Dioxide Extracts of Iberian Ham. J. Agric. Food Chem. 46, 5143-5150. doi:10.1021/jf980652v

Toldrá F, Flores M, Sanz Y. 1997. Dry-cured ham flavour: Enzymatic generation and process influence. Food Chem. 59, 523-530. doi:10.1016/S0308-8146(97)00013-7

Toldrá F, Flores M. 1998. The role of muscle proteases and lipases in flavor development during the processing of dry-cured ham. Crit. Reviews Food Sci. Nutri. 38, 331-352. doi:10.1080/10408699891274237

Toldrá F. 1998. Proteolysis and lipolysis in flavour development of dry-cured meat products. Meat Sci. 49, S101-S110.

Toldrá F. 2002. Dry-cured meat products. Wiley-Blackwell, Ames, Iowa, USA.

Toldrá F. 2004a. Curing: Dry, in Jensen W, Devine C, Dikemann M (Eds.), Encyclopedia of Meat Sciences, 360-365, Elsevier Science Ltd., London, UK.

Toldrá F. 2004b. Dry-cured ham. in: Hui YH, Goddik LM, Josephsen J, Stanfield PS, Hansen AS, Nip WK, Toldrá F (Eds.), Handbook of food and beverage fermentation technology, 369-384, Marcel-Dekker Inc., New York, USA.

Toldrá F, Flores M. 2004. Analysis of meat quality factors, in Nollet L (Ed.), Handbook of Food Analysis, 2nd edition, 1961-1977, Marcel-Dekker Inc., New York, USA.

Toldrá F. 2006a. Dry-cured ham, in: Hui YH, Castell-Perez E, Cunha LM, Guerrero-Legarreta I, Liang HH, Lo YM, Marshall DL, Nip WK, Shahidi F, Sherkat F, Winger RJ, Yam KL (Eds.), Handbook of Food Science, Technology and Engineering, vol 4, 164-1 to 164-11, CRC Press, Boca Raton, FL, USA.

Toldrá F. 2006b. The role of muscle enzymes in dry-cured meat products with different drying conditions. Trends Food Sci.Technol. 17, 164-168. doi:10.1016/j.tifs.2005.08.007

Toldrá F. 2007. Ham, in: Hui YH, Chandan R, Clark S, Cross N, Dobbs J, Hurst WJ, Nollet LML, Shimoni E, N Sinha, Smith EB, Surapat S, Titchenal A, Toldrá F (Eds.), Handbook of Food Product Manufacturing,Vol 2, 231-247, John Wiley Interscience, NY, USA.




How to Cite

Toldrá F, Aristoy M-C, Flores M. Relevance of nitrate and nitrite in dry-cured ham and their effects on aroma development. grasasaceites [Internet]. 2009Jul.30 [cited 2022Dec.7];60(3):291-6. Available from: