TY - JOUR AU - Tokuşoğlu, Özlem PY - 2008/06/30 Y2 - 2024/03/28 TI - Conjugated linoleic acid (CLA). Cis 9, trans 11 and trans 10, cis 12 isomer detection in crude and refined corn oils by capillary GC JF - Grasas y Aceites JA - Grasas aceites VL - 59 IS - 2 SE - Research DO - 10.3989/gya.2008.v59.i2.503 UR - https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/503 SP - 146-151 AB - Conjugated linoleic acids (CLAs) exhibit protective effects against various types of cancer and heart diseases. With the newly developed capillary gas chromatographic method (GC), <i>cis</i>9, <i>trans</i>11 and <i>trans</i>10, <i>cis</i>12 octadecadienoic acid isomers of CLA (C18:2) were determined in crude and refined corn oils as qualitative and quantitative measurements. <i>Cis</i> 9, <i>trans</i>11 C18:2 (<i>c</i>9, <i>t</i>11 CLA) was the major CLA isomer in both oils. It was found that c9, t11 CLA was 0.62% of the total lipid in crude oil and 1.24% of the total lipid in refined oil. Using the refining process, the total CLA was 1.38% whereas that of crude corn oil was 0.62%. An approximate 2.2 fold increase in the total CLA was found in refined oil (<i>n</i> = 9) (<i>p</i> < 0.01). The alteration in CLA could be used as a quality indicator for the refining process due to the contrubution of CLA to polyunsaturated fatty acids. Analytical method validation, calibration (R2 = 0.9999) and recovery data [<i>y</i> = 2.782x + 0.046 (R<sup>2</sup> = 0.9999)] were performed (<i>p</i> < 0.01).The proponed chromatographic procedure could be used for vegetable oil quality control ER -