TY - JOUR AU - Nieto, Susana AU - Sanhueza, Julio AU - Valenzuela, Alfonso PY - 1999/06/30 Y2 - 2024/03/28 TI - Synthesis of structured triacylglycerols containing medium-chain and long-chain fatty acids by interesterification with a stereoespecific lipase from Mucor miehei. JF - Grasas y Aceites JA - Grasas aceites VL - 50 IS - 3 SE - Research DO - 10.3989/gya.1999.v50.i3.656 UR - https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/656 SP - 199-202 AB - The preparation of structured triacylglycerols sn-1, sn-3 dilauryl, sn-2 eicosapentaenoyl glycerol and sn-1, sn-3 dilauryl, sn-2 docosahexaenoyl glycerol by enzymatic interesterification under restricted water availability is described. Laurie acid, one of the substrates for interesterification, was obtained by the controlled hydrolysis of coconut oil by a non-specific lipase obtained from <i>Candida cylindracea</i>. The fatty acid was separated from the hydrolysis products by silverresin column chromatography and converted to methyl ester, sn-2 Eicosapentaenoyl glycerol and sn-2 docosahexaenoyl glycerol were prepared by the hydrolysis of fish oil by the sn-1, sn-3 stereospecific immobilized lipase Lipozyme IM-20 obtained from <I>Mucor miehei</i> as described in the accompanying paper. The interesterification was carried out in a water jacketed glass reactor and the triacylglycerol products were separated and recovered through aluminum oxide column chromatography The interesterification procedure described allows to obtain In laboratory scale structured triacylglycerols containing medium-chain fatty acids at the sn-1 and sn-3 positions and long-chain polyunsaturated fatty acid from marine origin at the sn-2 glycerol position. ER -