Evaluation of the antioxidant activity of rice bran extracts using different antioxidant assays

Authors

  • Shahzad Ali Shahid Chatha Department of Chemistry, University of Agriculture, Faisalabad
  • Farooq Anwar Department of Chemistry, University of Agriculture, Faisalabad
  • Maleeha Manzoor Department of Chemistry, University of Agriculture, Faisalabad
  • Jawad -ur- Rehman Bajwa Department of Chemistry, University of Agriculture, Faisalabad

DOI:

https://doi.org/10.3989/gya.2006.v57.i3.56

Keywords:

Antioxidant activity, Antioxidant assays, Oxidation substrate, Percent inhibition, Total phenolics

Abstract


In the present work the antioxidant activity of different solvent (100% methanol, 80% methanol, 100% acetone, 80% acetone) extracts of rice bran was evaluated following different antioxidant assays and using sunflower oil as oxidation substrate. The rice bran extracts were evaluated from the estimate of % inhibition of peroxidation in linoleic acid system, total phenolics content (TPC) and loss of β-carotene in a linoleic acid system. Additionally, crude concentrated rice bran extracts were added into the sunflower oil samples and stored under ambient conditions. The extent of oxidative deterioration was followed by the measurement of peroxide-, p-anisidine-, conjugated diene-, and triene- values. The general order of antioxidant efficacy of rice bran extracts as determined by various antioxidant assays was 80% methanolic extract > 100% methanolic  extract > 80% acetone extract > 100% acetone extract. The results of the present comprehensive analysis demonstrate that rice bran extracts of the Super Kernel variety indigenous to Pakistan are a viable source of natural antioxidants and might be exploited for functional foods and nutraceutical applications.

Downloads

Download data is not yet available.

References

Amarowicz R, Karamac M and Chavan U. 2001. Influence of the extraction procedure on the antioxidative activity of lentil seed extracts in a beta-carotenelinoleate model system. Grasas Aceites. 52 (2): 89-93.

Anwar F, Bhanger M I, Yasmeen S. 2003. Antioxidant activity of some natural extracts in corn oil. Advanced Research of Plant Lipids, Proceeding of 15-ISPL, May 12-17, 2002, Okazaki, Japan. Eds. N. Muarata, M.Yamada, I.Nishida et al., Kluwer Publishers Netherlands, pp. 24-27.

Anwar F, Anwer T and Mahmood Z. 2005. Methodical characterization of rice (Oryza sativa) bran oil from Pakistan. Grasas Aceites. 56 (2): 126-135.

Awika JM, Rooney LW, Wu X, Prior RL and Zevallos LC. 2003. Screening methods to measure antioxidant activity of Surghum (Surghum bicolor) and Surghum products. Journal of Agriculture and Food Chemistry. 51, 6657-6662. doi:10.1021/jf034790i

Dekkers JC, Van Doornen LJP and Kemper HCG.1996. The role of antioxidant vitamins and enzymes in the prevention of exercise-induced muscles damage. Sports Medicine, 21, 213-238. doi:10.2165/00007256-199621030-00005

Halliwell B and Gutteridge JMC.1985. The chemistry of oxygen radicals and other oxygen derived species. In B. Halliwell and J. M. C. Gutteridge (Eds.), Free radicals in biology and medicine (pp. 20-64). New York: Oxford University Press.

Hou, D. X. 2003. Potential mechanism for cancer chemoprevention by anthocyanine. Current Advancements in Molecular Medicines.3: 149-159. doi:10.2174/1566524033361555

Iqbal S., Bhanger MI and Anwar F. 2005. Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan. Food Chemistry, 93: 265- 272. doi:10.1016/j.foodchem.2004.09.024

IUPAC, standard method for the analysis of Oils, Fats and Derivatives, Sixth Edition, Pergamon Press, Oxford, New York, Sydney, 1987. p. 20 – 118.

Jeong SM, Kim SY, Kim DR, Jo SC, Man KC, Ahn DU and Lee SC. 2004. Effect of heat treatment on the antioxidant activity of extracts from citrus peals. J. Agric. Food Chem., 52: 3389-3393. doi:10.1021/jf049899k

Laandrault N, Pouchert P, Ravel P, Gase F, Cros G and Teissedro PL.2001. Antioxidant activities and phenolics level of French wines from different varieties and vintages. J. Agric. Food Chem. 49, 3341- 3343. doi:10.1021/jf010128f

Lu Y and Foo LY.2000. Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chem. 68, 81-85. doi:10.1016/S0308-8146(99)00167-3

Malekain F, Rao RM, Prinyawiwatkul W, Marshall WE, Windhauser M and Ahmedna M.2000. Lipase and lipoxygenase activity, functionality and nutrient losses in rice bran during storage (pp. 1-68), Bulletin No. 870. Baton Rouge: LSU Agriculture center, Louisiana Agricultural experiment Station.

Mc Ginely L. 1991. Analysis and quality control for processing and processed fats. In: Analysis of Oilseeds, Fat and Fatty Food, Eds. Rossell JB; Printed, JLR, Elsevier Applied Science, London, New York, 1991, pp. 460-470.

Oryeneho SN and Hettiarachchy NS. 1992. Antioxidant activity of durum wheat bran. J. Agri. Food Chem.49: 1496-1500.

Osawa T and Namiki M.1981. A novel type of antioxidant isolated from leaf wax of eucalyptus leaves. Agriculture and Biological Chemistry, 45, 735-739.

Perumal S. and Becker K. 2003. Antioxidant properties of various solvent extracts of total phenolic constituents from three agroclimatic origins of Drumstick tree leaves. J. Agri. Food Chem. 51: 2144-2155. doi:10.1021/jf020444+

Prior RL.2004. Absorption and metabolism of anthocyanine: potential health effects. In M. Meskin, W.R. Bidlack, A. J. Devies, D. S. Lewis and R. K. Randolph (Eds.), Phytochemicals: mechanism of action (pp 1-9). Boca Raton, FL: CRC Press.

Rababah T. M., Hettiarachchy N. S. and Horex R. 2004. Total phenolics and antioxidant activities of fenugreek, green tea, black tea, grape seed, ginger, rosemary, gotu kola and ginkgo extracts, vitamin E and tert. Butylhydroquinone. J. Agri. Food Chem. 52: 5183- 5086. doi:10.1021/jf049645z

Sadddiq A, Anwar F, Manzoor M, Fatima A. 2005. Antioxidant activity of different solvent extracts of Moringa oleifera leaves under accelerated storage of sunflower oil. Asian J. of Plant Sci. 4(6): 630-635.

Shahidi F. 1997a. Natural antioxidants: An over view. In: F. Shahidi (Ed.), Natural antioxidant, Chemistry, Health Effects and Applications, (pp. 1-10). Champaign, IL, USA: AOCS press.

Shahidi F. 1997b. Natural antioxidants: An over view. In: F. Shahidi (Ed.), Natural antioxidant, Chemistry, Health Effects and Applications, (pp. 44-46). Champaign, IL, USA: AOCS press.

Steel R. G. D. and Torrie, J. H. (1980). Principles and procedures of statistics. London: McGraw Hill.

Velioglu Y. S., Mazza G., Gao L. and Oomah B. D. 1998. Antioxidant activity and total phenolics in selected fruits, vefetables ans grain products. J. Agri. Food Chem. 46: 4113-4117. doi:10.1021/jf9801973

Vichi S, Zitterl-Eseer, Jugl K and Franz MC. 2001. Determination of the presence of antioxidants derived from sage and organic extracts added to the animal fat by means of assessment of the radical scavenging capacity by photochemilumenescence analysis. Nahrung. 45 (2): 101-104. doi:10.1002/1521-3803(20010401)45:2<101::AID-FOOD101>3.0.CO;2-W

Wu X., Gu L., Holden J., Haytowitz D. B., Gebhardt S E., Beecher G. and Prior R. L. 2004. Development of a database for total antioxidant capacity in foods: a preliminary study. J. Food Composition and Analysis. 17: 407- 422. doi:10.1016/j.jfca.2004.03.001

Yen GC, Duh PD and Tsai CL. 1993. Relationship between antioxidant activity and maturity of peanut hull. Journal of Agriculture and Food Chemistry. 41, 67-70. doi:10.1021/jf00025a015

Zhou, K. and Yu, L. 2004. Antioxidant activities of bran extracts from “Trego” wheat grown at different locations. Journal of Agriculture and Food Chemistry. 52, 1112-1117. doi:10.1021/jf030621m

Downloads

Published

2006-09-30

How to Cite

1.
Shahid Chatha SA, Anwar F, Manzoor M, Rehman Bajwa J- ur-. Evaluation of the antioxidant activity of rice bran extracts using different antioxidant assays. Grasas aceites [Internet]. 2006Sep.30 [cited 2024Mar.28];57(3):328-35. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/56

Issue

Section

Research

Most read articles by the same author(s)

<< < 1 2