Antioxidant effect of an ozonized theobroma oil formulation on damaged-inflammatory rat skin


  • Yaima Sánchez Ozonized Vegetable Oils Group. Ozone Researches Center. CNIC
  • Maritza F. Díaz Ozonized Vegetable Oils Group. Ozone Researches Center. CNIC
  • Frank Hernández Ozonized Vegetable Oils Group. Ozone Researches Center. CNIC
  • Dayana Gil Ozonized Vegetable Oils Group. Ozone Researches Center. CNIC
  • Gastón García LIORAD Control Group of Biomedical Researches Laboratory



Antioxidant activity, Cosmetic formulation, Oxidant activity, Oxidative stress, Ozonized theobroma oil


The aim of this study was to determine whether a cosmetic formulation elaborated with ozonized theobroma oil may exert beneficial effects in the restoring of the antioxidant activity on the skin of rats previously irradiated with ultraviolet light. 0.5 g of the formulation was applied on the skin of rats for five days. Superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activity were determined in a homogenate of rat skin. Malondialdehyde (MDA), conjugated dienes (CD) and total hydroperoxide (THP) content were determined as biomarkers of oxidative stress. Using these parameters, antioxidant and oxidant activity, redox index and oxidative stress grade were determined. The total antioxidant activity was significantly increased while the redox index, total oxidant activity and oxidative stress grade decreased significantly in damaged rats treated with the formulation. These results show the antioxidant properties of the cosmetic formulation due to the stimulation of antioxidant enzymes such as SOD and GPx, preventing skin injury induced by ultraviolet irradiation.


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How to Cite

Sánchez Y, Díaz MF, Hernández F, Gil D, García G. Antioxidant effect of an ozonized theobroma oil formulation on damaged-inflammatory rat skin. grasasaceites [Internet]. 2011Mar.30 [cited 2023Feb.7];62(1):105-10. Available from: