Supercritical CO2 extraction of oil and omega-3 concentrate from Sacha inchi (Plukenetia volubilis L.) from Antioquia, Colombia




a -linolenic acid, Omega-3, Sacha inchi, Supercritical extraction


Sacha inchi (Plukenetia volubilis L.) seeds were employed for oil extraction with supercritical CO2 at laboratory scale. The supercritical extraction was carried out at a temperature of 60 °C, pressure range of 400–500 bars and CO2 flow of 40–80 g/min. The maximum recovery was 58% in 180 min, favored by increasing the residence time of CO2 in the extraction tank. Subsequently, the process was evaluated at pilot scale reaching a maximum recovery of 60% in 105 min, with a temperature of 60 °C, pressure of 450 bars and CO2 flow of 1270 g/min. The fatty acid composition of the oil was not affected for an extraction period of 30–120 min. The Sacha inchi oil was fractionated with supercritical CO2 to obtain an omega-3 concentrate oil without finding a considerable increase in the proportion of this compound, due to the narrow range in the carbon number of fatty acids present in the oil (16–18 carbons), making it difficult for selective separation.


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American Oil Chemists' Society. 1997. Sampling and analysis of commercial fats and oils. AOCS Official Method Ce 2-66: Preparation of Methyl Esters of Fatty Acids. Champaign, USA.

Chirinos R, Zuloeta G, Pedreschi R, Mignolet E, Larondelle Y, Campos D. 2013. Sacha inchi (Plukenetia volubilis): a seed source of polyunsaturated fatty acids, tocopherols, phytosterols, phenolic compounds and antioxidant capacity. Food Chem. 141, 1732–1739. PMid:23870885

Chrastil J. 1982. Solubility of solids and liquids in supercritical gases. J. Phys. Chem. 86, 3016–3021.

Fanali C, Dugo L, Cacciola F, Beccaria M, Grasso S, Dachà M, Mondello L. 2011. Chemical characterization of Sacha inchi (Plukenetia volubilis L.) oil. J. Agric. Food Chem. 59, 13043–13049. PMid:22053706

Follegatti-Romero L, Piantino CR, Grimaldi R, Cabral F. 2009. Supercritical CO2 extraction of omega-3 rich oil from Sacha inchi (Plukenetia volubilis L.) seeds. J. Supercrit. Fluids. 49, 323–329.

Guclu-Ustundag O, Temelli F. 2000. Correlating the Solubility Behavior of Fatty Acids, Mono-, Di-, and Triglycerides, and Fatty Acid Esters in Supercritical Carbon Dioxide. Ind. Eng. Chem. Res. 39, 4756–4766.

Guillén MD, Ruiz A, Cabo N, Chirinos R, Pascual G. 2003. Characterization of Sacha inchi (Plukenetia volubilis L.) oil by FTIR spectroscopy and 1H NMR. Comparison with linseed oil. J. Am. Oil Chem. Soc. 80, 755–762.

Gutiérrez LF, Rosada LM, Jiménez, Á. 2011. Chemical composition of Sacha Inchi (Plukenetia volubilis L.) seeds and characteristics of their lipid fraction. Grasas Aceites 62, 76–83.

International Organization for Standardization. 2009a. ISO3961: Animal and Vegetable fats and oils. Determination of iodine value. Geneva: ISO.

International Organization for Standardization. 2009b. ISO660: Animal and vegetable fats and oils. Determination of acid value and acidity. Geneva: ISO.

Markom M, Singh H, Hasan M. 2001. Supercritical CO2 fractionation of crude palm oil. J. Supercrit. Fluids. 20, 45–53.

Martínez J, De Aguiar AC. 2014. Extraction of Triacylglycerols and Fatty Acids Using Supercritical Fluids-Review. Curr. Anal. Chem. 10, 67–77.

Maurer NE, Hatta-Sakoda B, Pascual-Chagman G, Rodriguez- Saona LE. 2012. Characterization and authentication of a novel vegetable source of omega-3 fatty acids, Sacha inchi (Plukenetia volubilis L.) oil. Food Chem. 134, 1173–1180. PMid:23107745

Mezzomo N, Martínez J, Ferreira SRS. 2009. Supercritical fluid extraction of peach (Prunus persica) almond oil: Kinetics, mathematical modeling and scale-up. J. Supercrit. Fluids. 51, 10–16.

National Institute of Standards and Technology. 2011. Isothermal Properties for Carbon dioxide. Retrieved November 29, 2015, from fluid.cgi?T=40& PLow= 180&PHigh=350&PInc=170& Digits=5&ID=C124389 &Action=Load&Type=IsoThe rm&TUnit=C&PUnit= bar&DUnit=g%2Fml&HUnit= kJ%2Fmol&WUnit=m%2Fs&VisUnit=uPa*s&STUni t=N% 2Fm&RefState=DEF

Nilsson WB, Gauglitz EJ, Hudson JK. 1991. Solubilities of methyl oleate, oleic acid, oleyl glycerols, and oleyl glycerol mixtures in supercritical carbon dioxide. J. Am. Oil Chem. Soc. 68, 87–91.

Pereira De Souza AH, Gohara AK, Rodrigues ÂC, De Souza NE, Visentainer JV, Matsushita M. 2013. Sacha inchi as potential source of essential fatty acids and tocopherols: multivariate study of nut and shell. Acta Sci-Technol. 35, 757–763.

Prado JM, Dalmolin I, Carareto NDD, Basso RC, Meirelles AJA, Oliveira JV, Meireles MAA. 2012. Supercritical fluid extraction of grape seed: Process scale-up, extract chemical composition and economic evaluation. J. Food Eng. 109, 249–257.

R Core Team. 2014. R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing. Retrieved from http://www.r-project. org/

Rubio-Rodríguez N, Beltrán S, Jaime I, De Diego SM, Sanz MT, Carballido JR. 2010. Production of omega-3 polyunsaturated fatty acid concentrates: A review. Innov. Food Sci. Emerg. 11, 1–12.

Ruiz C, Díaz C, Anaya J, Rojas R. 2013. Análisis proximal, antinutrientes, perfil de ácidos grasos y de aminoácidos de semillas y tortas de 2 especies de Sacha inchi (Plukenetia volubilis y Plukenetia huayllabambana). Rev Soc Quím Perú. 79, 29–36. ISSN 1810-634X.

Shahidi F. 2005. Bailey's industrial oil and fat products. John Wiley & Sons, Inc., Publication, The USA, New Jersey.

Soares BMC, Gamarra FMC, Paviani LC, Gonçalves LAG, Cabral FA. 2007. Solubility of triacylglycerols in supercritical carbon dioxide. J. Supercrit. Fluids. 43, 25–31.

Staunton E, Todd W, Mason H, Van Bruggen J. 1969. Bioquímica Médica. Editorial Interamericana, S.A, Mexico.

Uquiche E, Fica X, Salazar K, Del Valle JM. 2012. Time Fractionation of Minor Lipids from Cold-Pressed Rapeseed Cake Using Supercritical CO2. JAOCS. 89, 1135–1144.



How to Cite

Triana-Maldonado DM, Torijano-Gutiérrez SA, Giraldo-Estrada C. Supercritical CO2 extraction of oil and omega-3 concentrate from Sacha inchi (Plukenetia volubilis L.) from Antioquia, Colombia. grasasaceites [Internet]. 2017Mar.30 [cited 2023May28];68(1):e172. Available from: