GC-MS analysis of volatiles in cinnamon essential oil extracted by different methods
Keywords:Cinnamic aldehyde, Cinnamon essential oil, Extraction method, GC-MS, Volatiles
Cinnamon essential oil (CEO) was extracted by three different methods: steam distillation (SD), ultrasound-assisted steam distillation (UASD) and microwave-assisted steam distillation (MASD). The volatiles in CEO were separated and identified by gas chromatography–mass spectrometry (GC-MS), and the differences in volatiles among the three different methods were further analyzed through principal component analysis. The results showed that 36 individual volatile components were present in the CEO from the three different methods. In general, the numbers of aldehydes, esters, alcohols, terpenes, aromatics and ketones were 6, 3, 7, 17, 2, and 1, respectively. The most abundant volatile component was determined to be cinnamic aldehyde. The content of total cinnamic aldehydes, which determines the price of CEO, was the highest among the three methods in the UASD sample (85.633%). Moreover, the highest yield (8.33‰) of essential oil was extracted by the UASD method. Therefore, UASD was the best way for CEO extraction in this research and was recommended for future industrial applications.
Ali SM, Khan AA, Ahmed I, Musaddiq M, Ahmed KS, Polasa, H. 2005. Antimicrobial activities of Eugenol and Cinnamaldehyde against the human gastric pathogen Helicobacter pylori. Ann. Clin. Microbiol. Antimicrob. 4, 20. https://doi.org/10.1186/1476-0711-4-20 PMid:16371157 PMCid:PMC1373661
Alonso A, Vázquez-Araújo L, García-Martínez S, Ruiz JJ, Carbonell-Barrachina ÁA. 2009. Volatile compounds of traditional and virus-resistant breeding lines of Muchamiel tomatoes. Eur. Food Res. Technol. 230, 315-323. https://doi.org/10.1007/s00217-009-1173-2
Azlina, MF, Hasfalina CM, Zurina ZA, Hishamuddin, J. 2013. Optimization and kinetic study of gaharu oil extraction. Int. Sch. Sci. Res. Innov. 7, 454-457.
Chang KS, Tak JH, Kim SI, Lee WJ, Ahn YJ. 2010. Repellency of Cinnamomum cassia bark compounds and cream containing cassia oil to Aedesaegypti (diptera: culicidae) under laboratory and indoor conditions. Pest. Manag. Sci. 62, 1032-1038. https://doi.org/10.1002/ps.1268 PMid:16894642
Chang ST, Chen PF, Chang SC. 2001. Antibacterial activity of leaf essential oils and their constituents from Cinnamomum osmophloeum. J. Ethnopharmacol. 77, 123-127. https://doi.org/10.1016/S0378-8741(01)00273-2
Cravotto G, Boffa L, Mantegna S, Perego P, Avogadro M, Cintas P. 2008. Improved extraction of vegetable oils under high-intensity ultrasound and/or microwaves. Ultrason. Sonochem. 15, 898-902. https://doi.org/10.1016/j.ultsonch.2007.10.009 PMid:18093864
Golmakani MT, Rezaei K. 2010. Microwave-assisted hydrodistillation of essential oil from zataria multiflora boiss. Eur. J. Lipid Sci. Technol. 110, 448-454. https://doi.org/10.1002/ejlt.200700239
Hong JK, Yang HJ, Jung H, Yoon DJ, Sang MK, Jeun YC. 2015. Application of volatile antifungal plant essential oils for controlling pepper fruit anthracnose by Colletotrichum gloeosporioides. Plan. Pathol. J. 31, 269-277. https://doi.org/10.5423/PPJ.OA.03.2015.0027 PMid:26361475 PMCid:PMC4564152
Jayatilaka A, Poole SK, Poole CF, Chichila TM. 1995. Simultaneous micro steam distillation/solvent extraction for the isolation of semivolatile flavor compounds from cinnamon and their separation by series coupled-column gas chromatography. Anal.Chim. Acta 302, 147-162. https://doi.org/10.1016/0003-2670(94)00445-R
Jeyaratnam N, Nour AH, Kanthasamy R, Nour AH, Yuvaraj AR, Akindoyo JO. 2016. Essential oil from Cinnamomum cassia bark through hydrodistillation and advanced microwave assisted hydrodistillation. Ind. Crops Prod. 92, 57-66. https://doi.org/10.1016/j.indcrop.2016.07.049
Kordali S, Cakir A, Ozer H, Cakmakci R, Kesdek M, Mete E. 2008. Antifungal, phytotoxic and insecticidal properties of essential oil isolated from Turkish Origanum acutidens and its three components, carvacrol, thymol and p-cymene. Bioresour. Technol. 99, 8788-8795. https://doi.org/10.1016/j.biortech.2008.04.048 PMid:18513954
Li R, Wang Y, Jiang ZT, Jiang S. 2010. Chemical composition of the essential oils of Cinnamomum loureirii Nees. From China obtained by hydrodistillation and microwave-assisted hydrodistillation. J. Essent. Oil Res. 22, 129-131. https://doi.org/10.1080/10412905.2010.9700281
Li YQ, Kong DX, Wu H. 2013. Analysis and evaluation of essential oil components of cinnamon barks using GC- MS and FTIR spectroscopy. Ind. Crops Prod. 41, 269-278. https://doi.org/10.1016/j.indcrop.2012.04.056
Lopez P, Sanchez C, Batlle R, Nerín C. 2007. Vapor-phase activities of cinnamon, thyme, and oregano essential oils and key constituents against foodborne microorganisms. J. Agric. Food Chem. 55, 4348-4356. https://doi.org/10.1021/jf063295u PMid:17488023
Luna G, Morales MT, Aparicio R. 2006. Characterisation of 39 varietal virgin olive oils by their volatile compositions. Food Chem. 98, 243-252. https://doi.org/10.1016/j.foodchem.2005.05.069
Matan N, Rimkeeree H, Mawson AJ, Chompreeda P, Haruthaithanasan V, Parker M. 2006. Antimicrobial activity of cinnamon and clove oils under modified atmosphere conditions. Int. J. Food Microbiol. 107, 180-185. https://doi.org/10.1016/j.ijfoodmicro.2005.07.007 PMid:16266767
Nabavi S, Di LA, Izadi M, Sobarzo-Sánchez E, Daglia, M. 2015. Antibacterial effects of cinnamon: From farm to food, cosmetic and pharmaceutical industries. Nutrients 7, 7729-7748. https://doi.org/10.3390/nu7095359 PMid:26378575 PMCid:PMC4586554
Ooi LS, Li Y, Kam SL, Wang H, Wong EY, Ooi VE. 2006. Antimicrobial activities of cinnamon oil and cinnamaldehyde from the Chinese medicinal herb Cinnamomum cassia Blume. Am. J. Chin. Med. 34, 511-522. https://doi.org/10.1142/S0192415X06004041 PMid:16710900
Park IK, Kim J, Lee SG, Shin SC. 2007. Nematicidal activity of plant essential oils and components from ajowan (Trachyspermum ammi), allspice (Pimentadioica) and litsea (Litseacubeba) essential oils against pine wood nematode (Bursaphelenchus xylophilus). J. Nematol. 39, 275-279.
Rui W, Wang R, Bao Y. 2009. Extraction of essential oils from five cinnamon leaves and identification of their volatile compound compositions. Innovative Food Sci. Emerg. Technol. 2, 289-292. https://doi.org/10.1016/j.ifset.2008.12.002
Singh G, Maurya S, Delampasona MP, Catalan CAN. 2007. A comparison of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents. Food Chem. Toxicol. 45, 0-1661. https://doi.org/10.1016/j.fct.2007.02.031 PMid:17408833
Wong HY, Tsai KD, Liu, YH, Yang SM, Chen TW, Cherng J, Cherng JM. 2016. Cinnamomum verum component 2-methoxycinnamaldehyde: A novel anticancer agent with both anti-topoisomerase I and II activities in human lung adenocarcinoma a549 cells in vitro and in vivo. Phytother. Res. 30, 331-340. https://doi.org/10.1002/ptr.5536 PMid:26676220
Wong YC, Ahmad-Mudzaqqir MY, Wan-Nurdiyana WA. 2014. Extraction of essential oil from cinnamon (Cinnamomum zeylanicum). Orient J. Chem. 30, 37-47. https://doi.org/10.13005/ojc/300105
Yu LI, Huang GC. 2007. Microwave assisted synthesis and application of cinnamic esters on packaging materials. Packaging Eng. 28, 34-36
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