Use of molecular methods for the identification of yeast species isolated from fermentations of table olives produced traditionally in Kahramanmaraş




Fermentation, Molecular identification, Salt, Table olive, Traditional, Yeast


In this study, yeast species involved in the naturally fermented green table olive produced in Southern Turkey were investigated. Table olive samples were prepared with regional olive cultivars and traditional methods were employed in the production. Yeasts were isolated from the brines of the samples at the late fermentation stage and identified at the species level by the DNA sequences of the D1/D2 domain of 26S rRNA genes. The D1/D2 domains were amplified by PCR, sequenced and compared to reference sequences deposited in the NCBI database. According to the results, isolated yeasts belonged to the CandidaDebaryomyces, and Rhodotorula genera and salt tolerant species were dominant as the salt content of the brines exceeded 11%. Among the determined species, Candida oleophila was the most dominant one and it was thought that isolated strains of Candida oleophila may be taken into consideration to be used as starter culture in table olive production.


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Alves M, Goncalves T, Quintas C. 2012. Microbial quality and yeast population dynamics in cracked green table olives' fermentations. Food Control 23 (2), 363-368.

AOAC. 1990. Official Methods of Analysis of the Association of Official Analytical Chemists. (Hekrichk, Editor), Vol: 1 and Vol:2, 15th edn, Arlington, Virginia 22201 USA.

Arroyo-López FN, Romero-Gil V, Bautista-Gallego J, Rodríguez-Gómez F, Jiménez-Díaz R. 2012a. Yeasts in table olive processing: Desirable or spoilage microorganisms? Int. J. Food Microbiol. 160 (1), 42-49. PMid:23141644

Arroyo-López FN, Romero-Gil V, Bautista-Gallego J. 2012b. Potential benefits of the application of yeast starters in table olive processing. Front Microbiol. 3, 1-4. PMid:22558000 PMCid:PMC3338231

Bautista-Gallego J, Rodríguez-Gómez F, Barrio E, Querol A. 2011. Exploring the yeast biodiversity of green table olive industrial fermentations for technological applications. Int. J. Food Microbiol. 147 (2), 89-96. PMid:21497408

Bleve G, Tufariello M, Durante M, Grieco F, Ramires FA, Mita G, Logrieco AF. 2015. Physico-chemical characterization of natural fermentation process of Conservolea and Kalamàta table olives and developement of a protocol for the pre-selection of fermentation starters. Food Microbiol. 46, 368-382. PMid:25475307

Cardinali F, Ferrocino I, Milanović V, Belleggia L, Corvaglia MR, Garofalo C, Foligni R, Mannozzi C, Mozzon M, Cocolin L, Osimani A, Aquilanti L. 2021. Microbial communities and volatile profile of Queijo de Azeitão PDO cheese, a traditional Mediterranean thistle-curdled cheese from Portugal. Food Res Int. 147, 110537. PMid:34399514

Corsetti A, Perpetuini G, Schirone M, Tofalo R, Suzzi G. 2012. Application of starter cultures to table olive fermentation: An overview on the experimental studies. Frontiers Microbiol. 3, 248.

da Silva GA, Bernardi TL, Schaker PDC, Menegotto M, Valente P. 2012. Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification. Braz. Arch. Biol. Technol. 55 (2), 319-327.

Deak T. 2008. Handbook of Food Spoilage Yeasts. (T. Deak, Ed.) (2nd ed.). Boca Raton: CRC Press.

Erten H, Tanguler H. 2014. Fermente Bitkisel Ürünler. In N. Aran (Ed.), Gıda Biyoteknolojisi 5., pp. 244-279. Ankara, Nobel.

Fadda ME, Mossa V, Pisano MB, Deplano M, Cosentino S. 2004. Occurrence and characterization of yeasts isolated from artisanal Fiore Sardo cheese. Int. J. Food Microbiol. 95 (1), 51-59. PMid:15240074

FAO. 2021. Faostat database search results (erişim tarihi 24/08/2021)

Hernández A, Martín A, Aranda E, Pérez-Nevado F, Córdoba MG. 2007. Identification and characterization of yeast isolated from the elaboration of seasoned green table olives. Food Microbiol. 24 (4), 346-351. PMid:17189760

Hurtado A, Reguant C, Esteve-Zarzoso B, Bordons A, Rozès N. 2008. Microbial population dynamics during the processing of Arbequina table olives. Food Res. Internat. 41 (7), 738-744.

Kara GN, Özbaş ZY. 2013. Importance of the Natural Yeast Flora in Table Olive Production. Gıda 38 (6), 375-382.

Kurtzman CP, Robnett CJ. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73 (4), 331-371. PMid:9850420

Leventdurur S, Sert-Aydın S, Boyacı-Gunduz P, Agirman B, Ghorbal A, Francesca N Erten H. 2016. Yeast biota of naturally fermented black olives in different brines made from cv. Gemlik grown in various districts of the Cukurova region of Turkey. Yeast 33, 289-301. PMid:27144328

Muccilli S, Caggia C, Randazzo CL, Restuccia C. 2011. Yeast dynamics during the fermentation of brined green olives treated in the field with kaolin and Bordeaux mixture to control the olive fruit fly. Int J Food Microbiol. 148 (1), 15-22. PMid:21570143

Mujdeci G, Arévalo-Villena M, Ozbas ZY, Briones Pérez A 2018. Yeast Identification During Fermentation of Turkish Gemlik Olives. J Food Sci. 83 (5), 1321-1325. PMid:29668115

Nisiotou A, Chorianopoulos N, Nychas GE, Panagou EZ 2010. Yeast heterogeneity during spontaneous fermentation of black Conservolea olives in different brine solutions. J. Appl. Microbiol. 108, 396-405. PMid:20438554

Ozturk I. 2015. Presence, changes and technological properties of yeast species during processing of pastirma, a Turkish dry-cured meat product. Food Control 50, 76-84.

Ozturk I, Sagdic O. 2014. Biodiversity of yeast mycobiota in "sucuk," a traditional turkish fermented dry sausage: Phenotypic and genotypic identification, functional and technological properties. J. Food Sci. 79 (11), M2315-M2322. PMid:25273925

Pereira EL, Ramalhosa E, Borges A, Pereira JA, Baptista P. 2015. Yeast dynamics during the natural fermentation process of table olives (Negrinha de Freixo cv.). Food Microbiol. 46, 582-586. PMid:25475331

Porru C, Rodríguez-Gómez F, Benítez-Cabello A, Jiménez-Díaz R, Zara G, Budroni M, Arroyo-López FN 2018. Genotyping, identification and multifunctional features of yeasts associated to Bosana naturally black table olive fermentations. Food Microbiol. 69, 33-42. PMid:28941907

Ruiz-Moyano S, Esperilla A, Hernández A, Benito MJ, Casquete R, Martín-Vertedor D, Pérez-Nevado F. 2019. Application of ISSR-PCR as a rapid method for clustering and typing of yeasts isolated from table olives. LWT. 109, 250-254.

Silva T, Reto M, So, M, Peito A, Peres CM, Peres C, Malcata, FX. 2011. Characterization of yeasts from Portuguese brined olives, with a focus on their potentially probiotic behavior. LWT-Food Sci. Technol. 44 (6), 1349-1354.

Tassou CC, Panagou EZ, Katsaboxakis KZ 2002. Microbiological and physicochemical changes of naturally black olives fermented at different temperatures and NaCl levels in the brines. Food Microbiol. 19 (6), 605-615.

Tofalo R, Perpetuini G, Schirone M, Suzzi G, Corsetti A. 2013. Yeast biota associated to naturally fermented table olives from different Italian cultivars. Int. J. Food Microbiol. 161 (3), 203-208. PMid:23334098

Vinicius De Melo Pereira G, De Carvalho Neto DP, Junqueira ACDO, Karp SG, Letti L AJ, Magalhães Júnior AI, Soccol CR 2020. A Review of Selection Criteria for Starter Culture Development in the Food Fermentation Industry. Food Reviews Int. 36 (2), 135-167.



How to Cite

Ayman S, Gezginc Y. Use of molecular methods for the identification of yeast species isolated from fermentations of table olives produced traditionally in Kahramanmaraş. Grasas aceites [Internet]. 2023Nov.24 [cited 2024Jul.24];74(4):e523. Available from:




Funding data

Kahramanmaraş Sütçü Imam Üniversitesi
Grant numbers 2016-6/25YLS