Evaluation of tobacco seed oil as a sustainable ingredient in oil-in-water (O/W) emulsion creams
DOI:
https://doi.org/10.3989/gya.0541241.2187Keywords:
Cosmetic, Emulsion, Moisturizin, Tobacco seed oilAbstract
Tobacco seeds, which are abundant and rich in oil, offer a sustainable alternative for cosmetic applications due to their fatty acid profile, which is similar to grape seed oil. This study compares tobacco seed oil (Krumovgrad 58 variety, Bulgaria) with commercial grape seed oil for use in cosmetic emulsions. Fatty acid composition and tocopherol content were analyzed, and emulsions were evaluated for physicochemical properties (peroxide value, acid value, refractive index, oxidative stability, and pH) and microbiological quality. The results showed that the emulsions had a moisture content of 82.2–85.1%, refractive index of 1.624–1.675, and oxidative stability of 2.60–3.85 hours. A microbiological analysis revealed fewer than 10 cfu/g of microorganisms, including molds and yeasts, in all emulsions. The findings demonstrate that tobacco seed oil exhibits comparable properties to grape seed oil, making it a viable, sustainable option for cosmetic formulations.
Downloads
References
Alvarez MR, Rodriguez ML. 2000. Lipids in pharmaceutical and cosmetic preparations. Grasas Aceites. 51 (1-2), 74-96. https://doi.org/10.3989/gya.2000.v51.i1-2.409
Ashirov MZ, Dathkayev UM, Myrzakozha DA, Hidetoshi S, Zhakipbekov KS, et al. 2020. Study of cold-pressed tobacco seed oil properties by gas chromatography method. Sci. World J. 2, 1-5. https://doi.org/10.1155/2020/8852724 PMid:33299385 PMCid:PMC7710410
Bialek A, Bialek M, Jelinska M, Tokarz A. 2016. Fatty acid profile of new promising unconventional plant oils for cosmetic use. Inter. J. Cosm. Sci. 38, 1-7. https://doi.org/10.1080/19476337.2016.1190406
Calvo F, Gómez JM, Ricardez-Sandoval L, Alvarez O. 2020. Integrated design of emulsified cosmetic products: A review. Chem. Eng. Res. Des. 161, 279-303. https://doi.org/10.1016/j.cherd.2020.07.014
Chao Ch, Génot C, Rodriguez C, Magniez H, Lacourt S, Fievez A, et al. 2018. Emollients for cosmetic formulations: Towards relationships between physico-chemical properties and sensory perceptions. Colloids Surf. A. 536, 156-164. https://doi.org/10.1016/j.colsurfa.2017.07.025
Farris PK. 2018. Chapter 5 - Topical Skin Care and the Cosmetic Patient. In Master Techniques in Facial Rejuvenation. 2nd edn. 68-72. https://doi.org/10.1016/B978-0-323-35876-7.00005-4
Gajinov Z, Matić M, Prćić S, Đuran V. 2010. Optical properties of the human skin. Serb. J. Dermatol. Venereol. 2 (4), 131-136. https://doi.org/10.2478/v10249-011-0029-5
Garavaglia J, Markoski MM, Oliveira A, Marcadenti A. 2016. Grape seed oil compounds: Biological and chemical actions for health. Nutr. Metab. Insights. 9, 59-64. https://doi.org/10.4137/NMI.S32910 PMid:27559299 PMCid:PMC4988453
Gavarkar P, Thorat S, Adnaik R, Mohite SK, Magdum Ch. 2016. Characterization and formulation of skin cream from seed oil extracted from Cucumis Melo. Sch. Res. Libr. Der Pharm. Lett. 8 (3), 90-93.
Gharby S, Harhar H, Roudani A, Chafchaouni I, Charrouf Z. 2013. Stability oxidative from cosmetic and alimentary argan oil оf thermal treatments. Inter. J. Pharm. Sci. Inv. 2 (5), 41-46.
Grisan S, Polizzotto R, Raiola P, Cristiani S, Ventura F, et al. 2016. Alternative use of tobacco as a sustainable crop for seed oil, biofuel, and biomass. Agron. Sustain. Dev. 36, 55. https://doi.org/10.1007/s13593-016-0395-5
Gu J, Zhang X, Song B, Zhou D, Niu Y, et al. 2022. Chemical Composition of Tobacco Seed Oils and Their Antioxidant, Anti-Inflammatory, and Whitening Activities. Molecules. 27, 23. https://doi.org/10.3390/molecules27238516 PMid:36500609 PMCid:PMC9739832
Hussein S, Abdrabba S. 2015. Physico-chemical Characteristics, Fatty Acid, Composition of Grape Seed Oil and Phenolic Compounds of Whole Seeds, Seeds and Leaves of Red Grape in Libya. Int. J. Appl. Sci. Mathem. 2 (5), 2394-2894.
ISO 659:2014. Oilseeds. Determination of oil content (Reference method), 2014.
ISO 3960:2007. (2007). Animal and vegetable fats and oils. Determination of Peroxide value.
ISO 12966-1:2014. Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters - Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters, 2014.
ISO 12966-2:2011. Animal and vegetable fat and oils. Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids, 2011.
ISO 9936:2016. Animal and vegetable fats and oils. Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography, 2016
ISO 6886:1996. Animal and vegetable fat and oils. Determination of oxidation stability (Accelerated oxidation test), 1996.
ISO 1149:2017. International Organization for Standardization. 2017. Enumeration and detection of aerobic mesophilic bacteria.
ISO 16212:2017. International Organization for Standardization. 2017. Cosmetics - Microbiology - Enumeration of yeast and mold.
ISO 18416:2015. International Organization for Standardization. 2015. Cosmetics - Microbiology - Detection of Candida albicans.
ISO 21150:2015. International Organization for Standardization. 2015. Cosmetics - Microbiology - Detection of Escherichia coli.
ISO 22717:2015. International Organization for Standardization. 2015. Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa.
ISO 22718:2015. International Organization for Standardization. 2015. Cosmetics - Microbiology - Detection of Staphylococcus aureus.
ISO 660:2009. (2009). Animal nad vegetable fats nad oils. Determination of acid and acidity. Geneva, Switzerland: ISO.
ISO 6886:2006. (2006). Animal and vegetable fats and oils. Determination of oxidative stability (Accelerated oxidation test). Geneva, Switzerland: ISO.
BDS EN ISO 6320:2000. (2000). Animal and vegetable fats and oils. Determination of refractive index. Kunik O, Saribekova D, Lazzara G, Cavallaro G. 2022. Emulsions based on fatty acid from vegetable oils for cosmetics. Ind. Crops Prod. 189, 115776. https://doi.org/10.1016/j.indcrop.2022.115776
Lukić M, Pantelić I, Savić SD. 2021. Towards Optimal pH of the Skin and Topical Formulations: From the Current State of the Art to Tailored Products. Cosmetics. 8, 69. https://doi.org/10.3390/cosmetics8030069
Mancuso A, Tarsitano M, Udongo, BP, Cristiano MCh. 2022. A comparison between silicone-free and silicone-based emulsions: Technological features and in vivo evaluation. Int. J. Cosmet. Sci. 44 (5), 514-529. https://doi.org/10.1111/ics.12800 PMid:35815903 PMCid:PMC9545630
Marshall T, Dosoky NS, Satyal P, Setzer WN. 2023. Aroma Compounds of Carrier Oils. Applied Chem. 3 (4), 546-580. https://doi.org/10.3390/appliedchem3040034
Maszewska M, Florowska A, Dłużewska E, Wroniak M, Marciniak-Lukasiak K, Żbikowska A. 2018. Oxidative Stability of Selected Edible Oils. Molecules. 23 (7), 1746. https://doi.org/10.3390/molecules23071746 PMid:30018226 PMCid:PMC6100155
Orchard A, Kamatou G, Viljoen AM, Patel N, Mawela P, Vuuren SFV. 2019. The influence of carrier oils on the antimicrobial activity and cytotoxicity of essential oils. Evid. Based Complement Alternat. Med. 6981305. https://doi.org/10.1155/2019/6981305 PMid:30733812 PMCid:PMC6348851
Popova V, Petkova Z, Ivanova T, Stoyanova M, Lazarov L, et al. 2018. Biologically active components in seeds of three Nicotiana species. Ind. Crops Prod. 117, 375-381. https://doi.org/10.1016/j.indcrop.2018.03.020
Selwyn A, Govindaraj S. 2023. Study of plant-based cosmeceuticals and skin care. South African J. Bot. 158, 429-442. https://doi.org/10.1016/j.sajb.2023.05.039
Shkreli R, Terziu R, Memushaj L, Dhamo K. 2022. Formulation and stability evaluation of a cosmetics emulsion loaded with different concentrations of synthetic and natural preservative. J. Biolog. Stud. 5 (1), 38-51. https://doi.org/10.62400/jbs.v5i1.6373
Srbinoska M, Filiposki K, Rafajlovska V. 2012. Fatty acid composition of tobacco seed oil and its potential as a source of linoleic acid. Int. Conf. Biosci. Biotechnol. Biodivers. Conf. Proc., 14, 164-167. https://docs.google.com/file/d/0B7aKJphx6o4lQW5nZGFhSHVZRDg/edit
Supriadi Y, Khoirin N. 2022. Formulation and Evaluation of Grape Seed Oil (Vitis Vinifera, L) Facial Cream with Variations in the Concentration of Stearic Acid as an Emulsifier. J Health Sci Med Dev.1 (01), 20-30. https://doi.org/10.56741/hesmed.v1i01.32
Symoniuk E, Ksibi N, Wroniak M, Lefek M, Ratusz K. 2022. Oxidative Stability Analysis of Selected Oils from Unconventional Raw Materials Using Rancimat Apparatus. Appl. Sci. 12 (20), 10355. https://doi.org/10.3390/app122010355
Zlatanov M, Angelova M, Antova G. 2007. Lipid composition of tobacco seeds. Bulg. J. Agric. Sci. 13, 539-544.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Consejo Superior de Investigaciones Científicas (CSIC)

This work is licensed under a Creative Commons Attribution 4.0 International License.
© CSIC. Manuscripts published in both the print and online versions of this journal are the property of the Consejo Superior de Investigaciones Científicas, and quoting this source is a requirement for any partial or full reproduction.
All contents of this electronic edition, except where otherwise noted, are distributed under a Creative Commons Attribution 4.0 International (CC BY 4.0) licence. You may read the basic information and the legal text of the licence. The indication of the CC BY 4.0 licence must be expressly stated in this way when necessary.
Self-archiving in repositories, personal webpages or similar, of any version other than the final version of the work produced by the publisher, is not allowed.
Funding data
European Commission
Grant numbers DUECOS BG-RRP-2.004- 0001-C01








