Chemical profile, antibacterial activity and antibiotic-modifying effects of the fixed oil of Annona squamosa L. (Annonaceae)

A.C.C.  Donelardy; D.O.D.  Leite; J.B.  do Nascimento; L.L.L.  Nascimento; F.F.G.  Rodrigues; J.W.G.  Castro; N.K.G.  de Carvalho; J.W.S.  Mendes; F.F. G-Rodrigues; J.G.M.  da Costa

doi:10.3989/gya.0435251.2320

Authors

A.C.C. Donelardy Department of Biological Chemistry, Natural Products Research Laboratory – LPPN, Regional University of Cariri https://orcid.org/0000-0002-6431-4901
D.O.D. Leite Department of Biological Chemistry, Natural Products Research Laboratory – LPPN, Regional University of Cariri https://orcid.org/0000-0003-3557-8366
J.B. do Nascimento Department of Biological Chemistry, Natural Products Research Laboratory – LPPN, Regional University of Cariri https://orcid.org/0000-0002-4124-4023
L.L.L. Nascimento Department of Biological Chemistry, Natural Products Research Laboratory – LPPN, Regional University of Cariri https://orcid.org/0000-0003-1087-9373
F.F.G. Rodrigues Department of Biological Chemistry, Natural Products Research Laboratory – LPPN, Regional University of Cariri https://orcid.org/0009-0001-3157-6518
J.W.G. Castro Department of Biological Chemistry, Natural Products Research Laboratory – LPPN, Regional University of Cariri https://orcid.org/0000-0002-5791-5880
N.K.G. de Carvalho Department of Biological Chemistry, Natural Products Research Laboratory – LPPN, Regional University of Cariri - Northeast Biotechnology Network – RENORBIO, State University of Ceará https://orcid.org/0000-0001-5325-3712
J.W.S. Mendes Department of Biological Chemistry, Natural Products Research Laboratory – LPPN, Regional University of Cariri https://orcid.org/0000-0001-7248-6466
F.F. G-Rodrigues Department of Biological Chemistry, Natural Products Research Laboratory – LPPN, Regional University of Cariri https://orcid.org/0000-0002-0137-9441
J.G.M. da Costa Department of Biological Chemistry, Natural Products Research Laboratory – LPPN, Regional University of Cariri- bNortheast Biotechnology Network – RENORBIO, State University of Ceará https://orcid.org/0000-0003-4268-663X

DOI:

https://doi.org/10.3989/gya.0435251.2320

Keywords:

Annona squamosa, Antibacterial activity, CG-MS, Elaidic acid

Abstract

Annona squamosa Linn (Annonaceae) is a medicinal plant used to treat various ailments such as urinary infections, diarrhea, and fever. The aim of this study was to determine the chemical profile and evaluate the antibacterial and antibiotic-modifying activities of the fixed oil from A. squamosa seeds (OFSAs). The antibacterial activity was evaluated by determining the Minimum Inhibitory Concentration (MIC) and antibiotic modulation with aminoglycosides. The chemical analysis of the OFSAS identified 21 fatty acid esters, with elaidic acid being the major compound, comprising 38.06% of the oil. The MIC antibacterial assay showed inhibitory effects and the modulation with aminoglycoside antibiotics demonstrated enhanced antibacterial activity. Statistical analysis of the modulation assay was performed using two-way ANOVA followed by Tukey’s post hoc test, with ****p < 0.0001. The use of OFSAs represents a promising perspective for application in antibacterial therapy, with further research needed, particularly regarding its mechanisms of action.

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