Obtaining procedure and determination of terpenic acids of olive leaf (Olea europaea)
Keywords:Oleanolic acid, Olive leaf, Terpenic acids
The terpenic acids, mainly the oleanolic acid, are used for the pharmaceutical industry, among other, for its antitumoral, anti-inflammatory and germicide activities. A process of obtainment of oleanolic acid from olive leaves is described. Solid/liquid extraction with ethanol, treatment of the extract and concentration under defined conditions, being possible to obtain in a single extraction stage a yield of 90 % of the leaf content (2-3 %, referred to the leaf weight) and with richness in excess of 95 %. The raw material is a by-product, coming from that of pruning yearly of the olive grove and of the cleaning of the olives. A method is described for the quantification of the terpenic acids from olive leaf of the alcoholic extract, by preparative thin layer chromatography fractionation and gas liquid chromatography quantification with betulinic acid standard.
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